福建地区鼻咽癌与人乳头状瘤病毒感染的相关性

发布时间：2018-08-18 21:09

【摘要】：目的:初步探讨中国福建地区鼻咽癌与人乳头状瘤病毒(HPV)感染的相关性。方法:收集经病理诊断明确为鼻咽癌的石蜡标本70例(鼻咽癌组),其中角化型鳞状细胞癌9例,非角化型癌61例(非角化分化型癌31例,非角化未分化型癌30例);以鼻咽部慢性炎症标本25例作为正常对照组。采用表面等离子谐振法(SPR)和聚合酶链反应法(PCR)检测鼻咽癌和正常组织中的HPV DNA;运用免疫组织化学方法检测鼻咽癌和正常组织中的HPV 16/18E6蛋白和EB病毒潜伏膜蛋白-1(LMP-1);运用原位杂交的方法检测EBER。结果:SPR和PCR法检测的70例鼻咽癌标本中有2例HPV DNA阳性,其中1例HPV-18阳性,为非角化未分化型癌;1例HPV-70阳性,为非角化分化型癌。2例HPV阳性标本中未检测出EBV相关指标。鼻咽癌标本中未检出HPV 16/18E6蛋白。70例鼻咽癌病理标本中EB病毒LMP-1的检出率为35.71%(25/70),EBER的检出率为81.43%(57/70)。正常对照组未检出HPV-DNA、HPV 16/18E6蛋白、LMP-1和EBER。结论:中国福建地区鼻咽癌中人乳头状瘤病毒感染率较低,该地区的鼻咽癌中无HPV和EB病毒共同感染现象。
[Abstract]:Objective: to investigate the relationship between nasopharyngeal carcinoma (NPC) and human papillomavirus (HPV) infection in Fujian province of China. Methods: seventy paraffin specimens of nasopharyngeal carcinoma (NPC group) were collected, including 9 cases of keratinized squamous cell carcinoma and 61 cases of non-keratinized carcinoma (31 cases of non-keratinizing differentiated carcinoma). 30 cases of non-keratinized undifferentiated carcinoma) and 25 cases of chronic inflammation of nasopharynx as normal control group. Surface plasma resonance (SPR) and polymerase chain reaction (PCR) were used to detect HPV in nasopharyngeal carcinoma and normal tissues, and HPV 16/18E6 protein and EBV latent membrane protein 1 (LMP-1) in nasopharyngeal carcinoma and normal tissues were detected by immunohistochemistry. EBER was detected by in situ hybridization. Results HPV DNA was positive in 2 of 70 nasopharyngeal carcinoma specimens detected by HPV DNA and PCR, of which 1 was HPV-18 positive, 1 was HPV-70 positive in non keratinized undifferentiated carcinoma, and no EBV related index was detected in 2 HPV positive samples of non keratinized differentiated carcinoma. EBV LMP-1 was detected in 35.71% (25 / 70) of NPC specimens and 81.43% (57 / 70) of EBERs. HPV-DNAA HPV 16/18E6 protein, LMP-1 and EBERwere not detected in normal control group. Conclusion: the infection rate of human papillomavirus (HPV) in nasopharyngeal carcinoma (NPC) in Fujian area of China is relatively low. There is no co-infection of HPV and EB virus in nasopharyngeal carcinoma in this area.
【作者单位】：南京军区福州总医院耳鼻咽喉头颈外科;
【分类号】：R739.63

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