Ruboxistaurin对小鼠氧诱导视网膜病变中新生血管抑制作用的形态学观察
发布时间：2018-08-19 13:52
【摘要】： 目的从形态学上探讨不同剂量Ruboxistaurin对氧诱导视网膜病变新生血管动物模型小鼠视网膜新生血管的作用 方法将出生7天的C57BL/6J小鼠随机分成：正常对照组,高氧模型组,药物处理组和空白对照组,除正常对照组之外,其余组放入氧分压为(75±2)%的氧箱内饲养5天后取出,然后药物治疗组小鼠经胃灌注不同剂量Ruboxistaurin,空白对照组灌注相同剂量甲磺酸盐,于鼠龄17天时将所有小鼠全部处死,取其眼球做标本固定,分别用计数突破视网膜内界膜的内皮细胞核数和偶氮蓝(Evans蓝)灌注血管造影法比较小鼠视网膜新生血管的情况。结果氧诱导视网膜病变模型鼠的视网膜中央及远周见无血管灌注区,周边部有大量新生血管形成；治疗组与高氧对照组和空白对照组相比,突破视网膜内界膜血管内皮细胞核明显减少,差别有显著意义(P0.01),视网膜新生血管和无灌注区较高氧组也明显好转。 结论氧诱导视网膜病变新生血管动物模型建立成功；氧诱导视网膜病变模型鼠视网膜新生血管和无灌注区较正常组增多；Ruboxistaurin能改善氧诱导视网膜病变小鼠视网膜新生血管的情况。
[Abstract]:Objective to investigate morphologically the effects of different doses of Ruboxistaurin on retinal neovascularization in mice with oxygen-induced retinopathy. Methods C57BL/6J mice were randomly divided into two groups: normal control group. In the hyperoxia model group, the drug treatment group and the blank control group, in addition to the normal control group, the rest groups were fed in oxygen box with oxygen partial pressure of (75 卤2)% for 5 days. Then the mice in the drug treatment group were perfused with different doses of Ruboxistaurin through the stomach and the control group were infused with the same dose of methanesulfonate. All the mice were killed at the age of 17 days and their eyeballs were fixed. The retinal neovascularization in mice was compared by counting the number of endothelial nuclei and Evans blue perfusion angiography. Results in the oxygen-induced retinopathy model rats, there were no vascular perfusion areas in the central and distal retina and a large number of neovascularization in the peripheral part of the retina, and the treatment group was compared with the hyperoxia control group and the blank control group. The number of endothelial nuclei in the retinal membranes was decreased significantly (P0.01). The retinal neovascularization and non-perfusion areas were also significantly improved compared with the hyperoxia group. Conclusion the animal model of oxygen-induced retinopathy neovascularization was established successfully, and the number of retinal neovascularization and non-perfusion area in oxygen-induced retinopathy model rats was more than that in normal group. Ruboxistaurin can improve the retinal neovascularization in mice with oxygen-induced retinopathy.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R774.1
本文编号：2191850
[Abstract]:Objective to investigate morphologically the effects of different doses of Ruboxistaurin on retinal neovascularization in mice with oxygen-induced retinopathy. Methods C57BL/6J mice were randomly divided into two groups: normal control group. In the hyperoxia model group, the drug treatment group and the blank control group, in addition to the normal control group, the rest groups were fed in oxygen box with oxygen partial pressure of (75 卤2)% for 5 days. Then the mice in the drug treatment group were perfused with different doses of Ruboxistaurin through the stomach and the control group were infused with the same dose of methanesulfonate. All the mice were killed at the age of 17 days and their eyeballs were fixed. The retinal neovascularization in mice was compared by counting the number of endothelial nuclei and Evans blue perfusion angiography. Results in the oxygen-induced retinopathy model rats, there were no vascular perfusion areas in the central and distal retina and a large number of neovascularization in the peripheral part of the retina, and the treatment group was compared with the hyperoxia control group and the blank control group. The number of endothelial nuclei in the retinal membranes was decreased significantly (P0.01). The retinal neovascularization and non-perfusion areas were also significantly improved compared with the hyperoxia group. Conclusion the animal model of oxygen-induced retinopathy neovascularization was established successfully, and the number of retinal neovascularization and non-perfusion area in oxygen-induced retinopathy model rats was more than that in normal group. Ruboxistaurin can improve the retinal neovascularization in mice with oxygen-induced retinopathy.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R774.1
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