鼻腔滴入重组IL-10对小鼠变应性鼻炎中CC10的表达调节
发布时间：2018-08-20 17:46
【摘要】：目的:研究Clara细胞1O-KDa蛋白( Clara cell 1O-KDa protein,CC10)在小鼠变应性鼻炎中的表达,探讨鼻腔滴入重组白介素10(interleukin-10,IL-10)对CC10表达调节。 方法:在第0、5天,用卵清蛋白(ovalbumin,OVA)对C57BL/6J小鼠进行腹腔注射来致敏,从第12至19天给予OVA行鼻腔激发,每天一次。治疗组分别用地塞米松(dexamethasone,DXM)和重组IL-10于鼻腔激发前行腹腔注射和鼻腔滴入,对照组用同样方法致敏,但是激发用无菌生理盐水代替OVA。分别用苏木素-伊红染色、过碘酸-希夫染色、免疫组织化学,检测小鼠鼻粘膜组织浸润的嗜酸性粒细胞、杯状细胞和CC10表达。 结果:对照组CC10主要由鼻中隔上纤毛上皮细胞和鼻甲上小圆顶状细胞分泌,而在OVA组表达显著下降(p 0.05),经过DXM或重组IL-10治疗,CC10表达明显上升,但是DXM作用要优于重组IL-10。和对照组相比,嗜酸性粒细胞和杯状细胞在DXM组和IL-10组增加,而纤毛上皮细胞和小圆顶状细胞减少,而在OVA组这种改变更加显著(p 0.05)。 结论:CC10可能作为一种内源性因子参与了变应性鼻炎的发病过程,并且能被外源性IL-10上调,但是作用机制不清楚,需要进一步研究。
[Abstract]:Aim: to study the expression of 1O-KDa protein (Clara cell 1O-KDa protein CC10 in Clara cells in mice with allergic rhinitis and to investigate the regulation of CC10 expression by nasal drip of recombinant interleukin-10 (IL-10). Methods: C57BL/6J mice were sensitized with ovalbumin OVA on day 0 to day 5. OVA was given nasal stimulation once a day from day 12 to day 19. In the treatment group, dexamethasone (DXM) and recombinant IL-10 were injected intraperitoneally and dripped into the nasal cavity before stimulation, while the control group was sensitized with the same method, but the aseptic saline was used instead of OVA. The expression of eosinophils goblet cells and CC10 in nasal mucosa of mice were detected by hematoxylin-eosin staining periodate-Schiff staining and immunohistochemistry. Results: in the control group, the expression of CC10 was mainly secreted by the epithelial cells of superior nasal septum ciliated and the small dome of the superior turbinate, but the expression of CC10 in the OVA group was significantly decreased (p0.05). The expression of CC10 was significantly increased after DXM or recombinant IL-10 treatment, but the effect of DXM was better than that of IL-10. Compared with the control group, eosinophil and goblet cells increased in DXM and IL-10 groups, but cilia epithelial cells and small domed cells decreased, but in OVA group the change was more significant (p0.05). Conclusion as an endogenous factor, the ratio CC10 may be involved in the pathogenesis of allergic rhinitis and can be up-regulated by exogenous IL-10, but the mechanism of action is not clear and needs further study.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R765.21
本文编号：2194481
[Abstract]:Aim: to study the expression of 1O-KDa protein (Clara cell 1O-KDa protein CC10 in Clara cells in mice with allergic rhinitis and to investigate the regulation of CC10 expression by nasal drip of recombinant interleukin-10 (IL-10). Methods: C57BL/6J mice were sensitized with ovalbumin OVA on day 0 to day 5. OVA was given nasal stimulation once a day from day 12 to day 19. In the treatment group, dexamethasone (DXM) and recombinant IL-10 were injected intraperitoneally and dripped into the nasal cavity before stimulation, while the control group was sensitized with the same method, but the aseptic saline was used instead of OVA. The expression of eosinophils goblet cells and CC10 in nasal mucosa of mice were detected by hematoxylin-eosin staining periodate-Schiff staining and immunohistochemistry. Results: in the control group, the expression of CC10 was mainly secreted by the epithelial cells of superior nasal septum ciliated and the small dome of the superior turbinate, but the expression of CC10 in the OVA group was significantly decreased (p0.05). The expression of CC10 was significantly increased after DXM or recombinant IL-10 treatment, but the effect of DXM was better than that of IL-10. Compared with the control group, eosinophil and goblet cells increased in DXM and IL-10 groups, but cilia epithelial cells and small domed cells decreased, but in OVA group the change was more significant (p0.05). Conclusion as an endogenous factor, the ratio CC10 may be involved in the pathogenesis of allergic rhinitis and can be up-regulated by exogenous IL-10, but the mechanism of action is not clear and needs further study.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R765.21
【参考文献】
相关期刊论文 前5条
1 ;IL-10-Producing Type 1 Regulatory T Cells and Allergy[J];Cellular & Molecular Immunology;2007年04期
2 吴红光;杨文东;;Clara细胞蛋白16和E-选择素与婴幼儿支气管哮喘及其相关性的临床研究[J];医学检验与临床;2007年06期
3 王海龙;庞敏;张彰;姜远虹;赵俊萍;梁宝华;;COPD大鼠Clara细胞超微结构及CC16的变化[J];山西医科大学学报;2007年02期
4 温志红,农生洲,胡琼燕,周微雅,杜华,陈芳,农利平;哮喘患儿Clara细胞分泌蛋白的临床意义[J];实用儿科临床杂志;2005年10期
5 桂芹,钱桂生,黄桂君,李淑萍;CC16基因G38A突变与汉族哮喘相关性的研究[J];中华医学遗传学杂志;2003年06期
，本文编号：2194481
本文链接：https://www.wllwen.com/yixuelunwen/yank/2194481.html
最近更新
教材专著
