MGMT、p53单核苷酸多态性与喉癌发病风险的研究
发布时间：2018-08-21 20:42
【摘要】：目的：探讨喉鳞状细胞癌与MGMT启动子区p53基因5’端上游序列单核苷酸多态性的相关性,探讨p53基因5’端上游序列单核苷酸多态性组成的两个单体型与喉癌的相关性,为喉鳞状细胞癌的发病提供遗传标记。方法：采用基因小测序法(SNaPshot法)检测96例喉鳞状细胞癌和102例对照人群的MGMT启动子区、p53基因5’端上游序列位点的4个单核苷酸多态性位点(rs1625649、rs2287499、rs2287498、rs228797)基因型,计算并比较两组人群基因型频率和等位基因频率,应用HaploView4.2计算rs2287499、rs2287498、rs228797连锁程度及单体型频率,对纳入对照人群的4个位点进行哈迪-温伯格平衡检验。实验所有数据采用SPSS17.0统计软件对实验数据采用卡方检验进行统计,检验水准a=0.05,设定p0.05为差异具有显著性。结果：MGMT启动子区和p53基因5’端上游序列位点的4个单核苷酸多态性位点rs1625649、rs2287499、rs2287498、rs228797在人群中均具有多态性,4个位点的基因型在病例组和健康对照组间差异没有统计学意义(X2值分别为4.47、0.98、1.67、4.68,P值均0.05),MGMT启动子区位点rs1625649的等位基因频率在病例组和健康对照组间差异有统计学意义(X2值为5.77,P0.05),p53基因5'端上游序列位点rs2287499、rs2287498、rs228797等位基因频率在病例组和健康对照组间差异没有统计学意义(X2值分别为1.11、1.56、3.36,P值均0.05)。rs2287499、rs2287498、rs228797可分为两种单体型,两种单体型在病例组和健康对照组间差异没有统计学意义(X2值为1.46,P值均0.05)结论：MGMT基因多态位与喉癌发病可能相关。
[Abstract]:Objective: to investigate the association between single nucleotide polymorphisms (SNP) of p53 gene 5'upstream sequence in laryngeal squamous cell carcinoma (LSCC) and MGMT promoter region, and to explore the relationship between the two haplotypes of p53 gene 5'upstream sequence mononucleotide polymorphism (HNP) and laryngeal carcinoma. To provide genetic markers for the development of laryngeal squamous cell carcinoma. Methods: four single nucleotide polymorphism loci (rs1625649, rs2287499 and rs2287498rs2287498rs2287498rs2287498rs2287498rs2287498rs2287498rs2287498) were detected in 96 cases of laryngeal squamous cell carcinoma (LSCC) and 102 controls by gene small sequencing (SNaPshot). Genotypic frequencies and allelic frequencies were calculated and compared between the two groups. The linkage degree and haplotype frequency of rs2287499 / rs228798 / rs228498 / rs228797 were calculated by HaploView4.2, and the Hardy Weinberg equilibrium test was performed at 4 loci in the control population. All the data of the experiment were statistically analyzed by using SPSS17.0 software. The test level was 0.05, and p0.05 was set as the significant difference. Results the four single nucleotide polymorphisms (rs1625649 / rs2287499 / rs2287498 / rs2287498rs228797) were found in the 5 '-terminal upstream of the 5' -terminal region of the p53 gene. There was no significant difference in the genotype of the 4 loci between the case group and the healthy control group. The allelic frequency of rs1625649 at the promoter site of MGMT was significantly different between the case group and the healthy control group (X2 = 5.77P 0.05). The allele frequency of the 5'upstream sequence of p53 gene rs2287499rs2287498rs228488rs228797 was significantly different between the case group and the healthy control group (X2 = 5.77-P0.05). There was no significant difference between healthy control group (X2 = 1.111.56 / 3.36 P = 0.05) .Rs2287499nrs2287498rs228797 could be divided into two haplotypes. There was no significant difference between the two haplotypes (X2 = 1.46, P = 0.05). Conclusion the polymorphic position of MGMT gene may be associated with laryngeal cancer.
【学位授予单位】：滨州医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.65
本文编号：2196295
[Abstract]:Objective: to investigate the association between single nucleotide polymorphisms (SNP) of p53 gene 5'upstream sequence in laryngeal squamous cell carcinoma (LSCC) and MGMT promoter region, and to explore the relationship between the two haplotypes of p53 gene 5'upstream sequence mononucleotide polymorphism (HNP) and laryngeal carcinoma. To provide genetic markers for the development of laryngeal squamous cell carcinoma. Methods: four single nucleotide polymorphism loci (rs1625649, rs2287499 and rs2287498rs2287498rs2287498rs2287498rs2287498rs2287498rs2287498rs2287498) were detected in 96 cases of laryngeal squamous cell carcinoma (LSCC) and 102 controls by gene small sequencing (SNaPshot). Genotypic frequencies and allelic frequencies were calculated and compared between the two groups. The linkage degree and haplotype frequency of rs2287499 / rs228798 / rs228498 / rs228797 were calculated by HaploView4.2, and the Hardy Weinberg equilibrium test was performed at 4 loci in the control population. All the data of the experiment were statistically analyzed by using SPSS17.0 software. The test level was 0.05, and p0.05 was set as the significant difference. Results the four single nucleotide polymorphisms (rs1625649 / rs2287499 / rs2287498 / rs2287498rs228797) were found in the 5 '-terminal upstream of the 5' -terminal region of the p53 gene. There was no significant difference in the genotype of the 4 loci between the case group and the healthy control group. The allelic frequency of rs1625649 at the promoter site of MGMT was significantly different between the case group and the healthy control group (X2 = 5.77P 0.05). The allele frequency of the 5'upstream sequence of p53 gene rs2287499rs2287498rs228488rs228797 was significantly different between the case group and the healthy control group (X2 = 5.77-P0.05). There was no significant difference between healthy control group (X2 = 1.111.56 / 3.36 P = 0.05) .Rs2287499nrs2287498rs228797 could be divided into two haplotypes. There was no significant difference between the two haplotypes (X2 = 1.46, P = 0.05). Conclusion the polymorphic position of MGMT gene may be associated with laryngeal cancer.
【学位授予单位】：滨州医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.65
【参考文献】
相关期刊论文 前1条
1 张军;王丽权;李唐英;;钬激光手术治疗早期声门型喉癌疗效分析[J];中国耳鼻咽喉头颈外科;2010年10期
，本文编号：2196295
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