肿瘤坏死因子-α及锌指蛋白36基因多态性与中重度阻塞性呼吸睡眠暂停综合征相关性研究

发布时间：2018-08-23 19:02

【摘要】：目的探讨锌指蛋白36(ZFP36)基因多态性与肿瘤坏死因子(TNF)-α及中重度阻塞性呼吸睡眠暂停综合征(OSAS)的相关性。方法应用TaqMan-PCR方法检测2010年1月至2012年7月在新疆维吾尔自治区人民医院高血压科住院的408例中重度OSAS患者(OSAS组)和194名健康体检者(对照组)的ZFP36 3个位点(rs251864、rs3746083及rs17879933)。采用放射免疫法检测TNF-α。结果 OSAS组的颈围、腹围、体重指数、收缩压、舒张压、血尿酸、总胆固醇及TNF-α均明显高于对照组(P0.05)。两组在ZFP36 rs251864和rs17879933位点的基因型和等位基因频率分布差异有统计学意义,TNF-α在上述2个位点的不同基因型差异有统计学意义(P0.05)。3个位点的不同基因型在呼吸睡眠相关指标中,差异均无统计学意义。Logistic回归分析结果显示,TNF-α不是OSAS的独立危险因素。在控制了年龄、性别等混杂因素后,未发现ZFP36 3个位点基因多态性与OSAS之间存在相关性。结论中重度OSAS患者TNF-α明显增高,同时ZFP36参与对TNF-α的调节,但ZFP36 3个位点的突变不是中重度OSAS发生的独立危险因素。
[Abstract]:Objective to investigate the association of zinc finger protein 36 (ZFP36) gene polymorphism with tumor necrosis factor (TNF)-伪 and moderate to severe obstructive sleep apnea syndrome (OSAS). Methods from January 2010 to July 2012, the three ZFP36 loci (rs251864,rs3746083 and rs17879933) of 408 moderate and severe OSAS patients (OSAS group) and 194 healthy persons (control group) were detected by TaqMan-PCR method in hypertension department of Xinjiang Uygur Autonomous region people's Hospital from January 2010 to July 2012. TNF- 伪 was detected by radioimmunoassay. Results the neck circumference, abdominal circumference, body mass index, systolic blood pressure, diastolic blood pressure, uric acid, total cholesterol and TNF- 伪 in OSAS group were significantly higher than those in control group (P0.05). There were significant differences in genotype and allele frequency distribution between the two groups at ZFP36 rs251864 and rs17879933 loci. There were significant differences in the genotypes of TNF- 伪 between the above two loci (P0.05). Logistic regression analysis showed that TNF- 伪 was not an independent risk factor for OSAS. After controlling for age, sex and other confounding factors, there was no correlation between ZFP36 gene polymorphism and OSAS. Conclusion TNF- 伪 is significantly increased in moderate and severe OSAS patients, and ZFP36 is involved in the regulation of TNF- 伪, but the mutation of three ZFP36 loci is not an independent risk factor for the occurrence of moderate and severe OSAS.
【作者单位】：新疆医科大学第一附属医院干部病房;新疆维吾尔自治区人民医院高血压科
【基金】：国家重点基础研究发展计划(973计划)(2012CB517802)
【分类号】：R766

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