P21、P27蛋白在下咽癌中的表达及其临床意义

发布时间：2018-08-24 10:13

【摘要】：目的研究P21、P27蛋白在下咽癌中的表达以及与不同的临床病理参数间的关系及临床意义。方法材料来自2009年10月到2010年6月山东大学齐鲁医院耳鼻喉科手术切除的26例标本,全部为梨状窝癌及相应的淋巴结。采用二步法免疫组化方法检测26例梨状窝癌及相应的淋巴结中P21、P27蛋白表达。P21、P27蛋白阳性表达主要在胞核中(P27部分在胞浆中有表达)呈棕黄色颗粒沉着,采用Greenpan半定量法对细胞的着色强度及阳性细胞的百分率进行评分。结果1.P21蛋白在下咽癌和阳性淋巴结转移癌中的表达率分别为34.62%和53.33%,P0.05。P27蛋白在下咽癌和阳性淋巴结转移癌中的表达率分别为73.08%和66.67%,P0.05。2.P21蛋白的表达与肿瘤的分化程度、淋巴结转移、性别差异有统计学意义,与临床分级、年龄差异无统计学意义。2.P27蛋白表达与年龄、肿瘤的分化程度中高分化与低分化差异有统计学意义,与临床分级、肿瘤的分化程度中高分化与中分化、中分化与低分化、淋巴结转移、性别差异无统计学意义。3.P21、P27蛋白在下咽癌中的表达差异有统计学意义。4.P21、P27蛋白在淋巴结转移癌中表达差异有统计学意义。结论本研究显示P21蛋白、P27蛋白在下咽癌及对应的阳性淋巴结转移癌中的表达正相关,P21蛋白、P27蛋白并未因转移癌表达上升或下降,从某方面证明下咽转移癌与原发癌临床病理特征相近。P21蛋白在下咽癌中的表达低于P27蛋白表达,说明二者表达的机制有差异。
[Abstract]:Objective to study the expression of P 21 p27 protein in hypopharyngeal carcinoma and its relationship with different clinicopathological parameters and its clinical significance. Methods from October 2009 to June 2010, 26 specimens were obtained from Otolaryngology, Qilu Hospital of Shandong University, all of which were Pyriform fossa carcinoma and corresponding lymph nodes. A two-step immunohistochemical method was used to detect the expression of P21 P27 protein in 26 cases of Pyriform fossa carcinoma and its corresponding lymph nodes. The positive expression of P21 P27 protein was mainly found in the nucleus (P27 part was expressed in the cytoplasm). The staining intensity and percentage of positive cells were evaluated by Greenpan semi-quantitative method. Results the expression rates of 1.P21 protein in hypopharyngeal carcinoma and positive metastatic lymph node carcinoma were 34.62% and 53.33%, respectively. The expression rates of P0.05.P27 protein in hypopharyngeal carcinoma and positive metastatic lymph node carcinoma were 73.08% and 66.67%, respectively. There was significant difference between sex and clinical grade. There was no significant difference in age. 2. The expression of P27 protein and age, the differentiation degree of tumor was significantly different between high differentiation and low differentiation, and the difference was statistically significant with clinical grade. The degree of differentiation of tumor is medium and high differentiation and medium differentiation, medium differentiation and low differentiation, lymph node metastasis. There was no significant difference in the expression of P27 protein in hypopharyngeal carcinoma. 4. There was significant difference in the expression of P21 protein in metastatic lymph node carcinoma. Conclusion the expression of P21 protein and P27 protein in hypopharyngeal carcinoma and the corresponding positive lymph node metastatic carcinoma were not increased or decreased due to the expression of P21 protein, which was positively correlated with the expression of P21 protein in hypopharyngeal carcinoma and the corresponding positive lymph node metastatic carcinoma. The expression of P21 protein in hypopharyngeal carcinoma was lower than that of P27 protein in hypopharyngeal carcinoma, which indicated that the mechanism of expression of P21 protein was different.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.63

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