天然免疫蛋白PLUNC在鼻息肉中的表达及与鼻息肉临床特征的关联性

发布时间：2018-08-25 07:17

【摘要】：目的:探讨天然免疫蛋白腭、肺、鼻上皮克隆(PLUNC)在鼻息肉组织中的表达情况,分析PLUNC蛋白浓度与鼻息肉大小(鼻内镜记分)和手术后复发的关联性。方法:采用免疫组织化学和实时定量PCR检测鼻息肉组(28例鼻息肉患者,其中13例为术后复发患者)和对照组(16例钩突黏膜对照)PLUNC的组织染色定位和mRNA的表达情况,同时采用ELISA检测初发和复发鼻息肉组织中PLUNC的蛋白浓度差异,评估PLUNC蛋白浓度与息肉大小、鼻塞、流涕症状记分的相关性。结果:鼻息肉组织中PLUNC主要定位在黏膜上皮和腺体,染色强度记分显著低于对照的钩突组织(P0.01);PLUNC mRNA表达水平也显著低于对照的钩突组织(P0.01)。初发和复发鼻息肉组织中PLUNC蛋白OD值分别为0.33±0.11和0.15±0.05,差异有统计学意义(P0.01)。小型和大型鼻息肉PLUNC蛋白OD值分别为0.32±0.14和0.19±0.07,差异有统计学意义(P0.05)。鼻息肉组织中PLUNC蛋白浓度与鼻塞和流涕记分存在显著负相关(r=-0.51和r=-0.57,P0.01)。结论:PLUNC的表达降低提示鼻息肉的发生可能与天然免疫反应的减弱有关,因此,上调天然免疫分子如PLUNC等的表达可作为干预鼻息肉发病的一个新策略。
[Abstract]:Objective: to investigate the expression of (PLUNC) in nasal polyps and analyze the relationship between PLUNC protein concentration and nasal polyp size (nasal endoscopic score) and recurrence after surgery. Methods: immunohistochemical and real-time quantitative PCR were used to detect the expression of PLUNC and mRNA in nasal polyps (28 cases of nasal polyps, 13 cases of recurrent nasal polyps) and control group (16 cases of uncinate mucosal control). At the same time, ELISA was used to detect the difference of PLUNC protein concentration in primary and recurrent nasal polyps, and to evaluate the correlation between PLUNC protein concentration and polyp size, nasal congestion and runny symptoms. Results: the expression of PLUNC in nasal polyps was mainly located in mucosal epithelium and glands, and the staining intensity score was significantly lower than that in the control (P0.01) and the expression level of PLUNC mRNA in the control (P0.01) was also significantly lower than that in the control (P0.01). The OD values of PLUNC protein in primary and recurrent nasal polyps were 0.33 卤0.11 and 0.15 卤0.05, respectively (P0.01). The OD values of PLUNC protein in small and large nasal polyps were 0.32 卤0.14 and 0.19 卤0.07, respectively (P0.05). The concentration of PLUNC protein in nasal polyps was negatively correlated with nasal congestion and runny score (r-0.51 and r-0.57, P0.01). Conclusion the decrease of the expression of fraction PLUNC suggests that the occurrence of nasal polyps may be related to the decrease of innate immune response. Therefore, upregulating the expression of innate immune molecules such as PLUNC may be a new strategy to interfere with the pathogenesis of nasal polyps.
【作者单位】：广州市中西医结合医院耳鼻咽喉科;广州市中山大学附属第一医院耳鼻咽喉科医院;
【基金】：国家自然科学基金项目(No:81271054)
【分类号】：R765.25

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