c-FLIP和RECK在喉鳞癌及癌旁组织中的表达及对于喉癌手术的指导意义
发布时间：2018-08-28 07:32
【摘要】： 目的：1,探讨癌基因c-FLIP和抑癌基因RECK在喉癌组织中的表达与喉癌临床特征的关系,了解喉癌的生物学行为。并从细胞和基因水平探讨c-FLIP和RECK在喉癌及癌旁不同距离粘膜组织中表达变化特征,分析两者作为喉癌特异性分子指标的可行性,并探讨喉癌手术粘膜安全切缘的范围。2,研究RECK因子在声门上喉癌中的表达特征,初步探索声门上喉癌粘膜下浸润的距离及深部切缘安全界的标准。 方法：1,分别采用PV9000免疫组织化学两步法及RT-PCR方法检测38例喉磷癌组织及距癌组织边缘2mm、5mm、10mm粘膜组织中c-FLIP和RECK蛋白的表达及mRNA转录情况,同时取距离癌组织边缘15mm处粘膜组织作为对照。2,应用免疫组化方法检测24例声门上喉鳞癌及粘膜下浸润不同距离的深部切缘组织中RECK蛋白的表达,并采用图像分析技术,计算蛋白表达的平均光密度值,结果采用均数±标准差(mean±SD)的形式表示,并进行方差分析,组间采用SNK检验。 结果：1, c-FLIP和RECK在喉鳞癌组织中的表达水平与患者临床分期和肿瘤病理学分级分别成正相关和负相关。c-FLIP在伴颈淋巴结转移组中的表达水平明显高于非转移组,而RECK则正好相反。2,c-FLIP表达情况依癌组织→癌旁2mm→癌旁5mm→癌旁10mm→癌旁15mm顺序递减,而RECK则递增。c-FLIP在癌组织与癌旁5mm、10mm、15mm组织之间表达水平有显著性差异(P0.05),而与癌旁2mm处组织之间差别无统计学意义。RECK在癌组织与癌旁5mm、10mm、15mm组织之间表达水平有显著性差异(P0.05),而与癌旁2mm处组织之间差别无统计学意义。3,RECK蛋白在声门上喉癌中的表达与病理分化程度及临床分期有密切关系。4,RECK蛋白的表达量在声门上喉癌及不同距离深部切缘组织中沿癌组织→2mm→5mm→10mm→15mm这样的顺序呈现出递增趋势。平均光密度值测定得出癌组织,2mm,5mm处组织三者之间表达无明显差别(P=0.088),10mm与15mm处组织表达也无差别(P=0.251),但前三者与后两者之间的表达量有显著差别(P0.01)。 结论：1,c-FLIP和RECK两因子在喉鳞癌组织中的表达水平与喉鳞癌患者的临床病理参数密切相关。2,c-FLIP和RECK可能都参与了喉鳞癌的发生、发展,并与喉鳞癌的浸润及转移有密切关系。3,c-FLIP和RECK在喉鳞癌组织中及癌旁各距离粘膜组织中表达情况进一步表明以距肿瘤边缘5mm作为粘膜安全切缘的标准较为适宜。4,声门上喉癌粘膜下深部切缘的安全切除范围可能达到0.5.1cm之间,有待于术后随访进一步证实。
[Abstract]:Objective to investigate the relationship between the expression of oncogene c-FLIP and tumor suppressor gene RECK and the clinical features of laryngeal carcinoma, and to understand the biological behavior of laryngeal carcinoma. The expression characteristics of c-FLIP and RECK in laryngeal carcinoma and adjacent mucosa tissues were studied from cell and gene levels, and the feasibility of using them as specific molecular markers of laryngeal carcinoma was analyzed. To study the expression of RECK factor in supraglottic laryngeal carcinoma, and to explore the distance of submucosal invasion of supraglottic laryngeal carcinoma and the standard of deep margin safety. Methods the expression of c-FLIP and RECK protein and the transcription of mRNA in 38 cases of laryngeal phosphorous carcinoma and 2mm ~ 5mm ~ 10mm from the margin of laryngeal carcinoma were detected by PV9000 immunohistochemical two-step method and RT-PCR method respectively. The expression of RECK protein in 24 cases of supraglottic laryngeal squamous cell carcinoma (SCSCC) and deep incised margin tissues with submucosal infiltration was detected by immunohistochemical method, and the expression of RECK protein was detected by image analysis technique in 24 cases of supraglottic laryngeal squamous cell carcinoma (SCSCC) and deep incised margin tissues with submucosal infiltration. The average optical density of protein expression was calculated. The results were expressed in the form of mean 卤standard deviation (mean 卤SD), analysis of variance and SNK test between groups. Results the expression levels of c-FLIP and RECK in laryngeal squamous cell carcinoma were positively and negatively correlated with clinical stage and tumor pathological grade, respectively. The expression of c-FLIP in patients with cervical lymph node metastasis was significantly higher than that in non-metastasis group. On the contrary, the expression of c-FLIP in RECK decreased according to the sequence of 15mm in adjacent 2mm and 5mm adjacent to 5mm. However, the expression of c-FLIP in cancer tissue was significantly higher than that in adjacent 2mm tissue (P0.05), but there was no significant difference between c-FLIP and adjacent 2mm tissue (P0.05). There was a significant difference in the expression level of reck between cancer tissue and adjacent 5mm ~ 10mm ~ 15mm tissue (P0.05). (P0.05), but there was no significant difference between the expression of Reck protein and adjacent 2mm tissues. 3The expression of Reck protein in supraglottic laryngeal carcinoma was closely related to the degree of pathological differentiation and clinical stage. The expression of Reck protein in supraglottic laryngeal carcinoma and deep resection at different distances was closely related to the degree of pathological differentiation and clinical stage. The sequence of 2mm 5mm 10mm 15mm in marginal tissue showed an increasing trend. The average optical density value showed that there was no significant difference between the three groups (P0. 088) and 15mm (P0. 251), but there was a significant difference between the first three and the latter (P0. 01). Conclusion the expression level of c-FLIP and RECK in laryngeal squamous cell carcinoma is closely related to the clinicopathological parameters of laryngeal squamous cell carcinoma. Both c-FLIP and RECK may be involved in the occurrence and development of laryngeal squamous cell carcinoma. The expression of c-FLIP and RECK in laryngeal squamous cell carcinoma tissues and adjacent mucosal tissues further indicated that 5mm from the edge of the tumor as the safe margin of mucous membrane is more suitable for .4. the expression of c-FLIP and RECK in laryngeal squamous cell carcinoma tissues and adjacent mucosa tissues is more suitable. The safe resection range of the deep incisal margin of suprahilar laryngocarcinoma may be between 0.5.1cm, To be further confirmed by postoperative follow-up.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65
本文编号：2208714
[Abstract]:Objective to investigate the relationship between the expression of oncogene c-FLIP and tumor suppressor gene RECK and the clinical features of laryngeal carcinoma, and to understand the biological behavior of laryngeal carcinoma. The expression characteristics of c-FLIP and RECK in laryngeal carcinoma and adjacent mucosa tissues were studied from cell and gene levels, and the feasibility of using them as specific molecular markers of laryngeal carcinoma was analyzed. To study the expression of RECK factor in supraglottic laryngeal carcinoma, and to explore the distance of submucosal invasion of supraglottic laryngeal carcinoma and the standard of deep margin safety. Methods the expression of c-FLIP and RECK protein and the transcription of mRNA in 38 cases of laryngeal phosphorous carcinoma and 2mm ~ 5mm ~ 10mm from the margin of laryngeal carcinoma were detected by PV9000 immunohistochemical two-step method and RT-PCR method respectively. The expression of RECK protein in 24 cases of supraglottic laryngeal squamous cell carcinoma (SCSCC) and deep incised margin tissues with submucosal infiltration was detected by immunohistochemical method, and the expression of RECK protein was detected by image analysis technique in 24 cases of supraglottic laryngeal squamous cell carcinoma (SCSCC) and deep incised margin tissues with submucosal infiltration. The average optical density of protein expression was calculated. The results were expressed in the form of mean 卤standard deviation (mean 卤SD), analysis of variance and SNK test between groups. Results the expression levels of c-FLIP and RECK in laryngeal squamous cell carcinoma were positively and negatively correlated with clinical stage and tumor pathological grade, respectively. The expression of c-FLIP in patients with cervical lymph node metastasis was significantly higher than that in non-metastasis group. On the contrary, the expression of c-FLIP in RECK decreased according to the sequence of 15mm in adjacent 2mm and 5mm adjacent to 5mm. However, the expression of c-FLIP in cancer tissue was significantly higher than that in adjacent 2mm tissue (P0.05), but there was no significant difference between c-FLIP and adjacent 2mm tissue (P0.05). There was a significant difference in the expression level of reck between cancer tissue and adjacent 5mm ~ 10mm ~ 15mm tissue (P0.05). (P0.05), but there was no significant difference between the expression of Reck protein and adjacent 2mm tissues. 3The expression of Reck protein in supraglottic laryngeal carcinoma was closely related to the degree of pathological differentiation and clinical stage. The expression of Reck protein in supraglottic laryngeal carcinoma and deep resection at different distances was closely related to the degree of pathological differentiation and clinical stage. The sequence of 2mm 5mm 10mm 15mm in marginal tissue showed an increasing trend. The average optical density value showed that there was no significant difference between the three groups (P0. 088) and 15mm (P0. 251), but there was a significant difference between the first three and the latter (P0. 01). Conclusion the expression level of c-FLIP and RECK in laryngeal squamous cell carcinoma is closely related to the clinicopathological parameters of laryngeal squamous cell carcinoma. Both c-FLIP and RECK may be involved in the occurrence and development of laryngeal squamous cell carcinoma. The expression of c-FLIP and RECK in laryngeal squamous cell carcinoma tissues and adjacent mucosal tissues further indicated that 5mm from the edge of the tumor as the safe margin of mucous membrane is more suitable for .4. the expression of c-FLIP and RECK in laryngeal squamous cell carcinoma tissues and adjacent mucosa tissues is more suitable. The safe resection range of the deep incisal margin of suprahilar laryngocarcinoma may be between 0.5.1cm, To be further confirmed by postoperative follow-up.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65
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