翼状胬肉组织VEGF、PCNA的表达及相关性研究

发布时间：2018-09-04 14:01

【摘要】：目的：通过研究血管内皮生长因子(VEGF)、增殖细胞核抗原(PCNA)在翼状胬肉组织和正常结膜组织中的表达及VEGF、PCNA相关性,探讨VEGF、PCNA与翼状胬肉之间的关系。材料与方法：选取2008年08月-2010年04月在暨南大学附属第一医院眼科行手术切除的翼状胬肉组织标本35例,正常结膜组织标本10例。常规HE染色行组织病理学检查和VEGF、PCNA免疫组织化学检查,分别观察其组织病理学表现和VEGF、PCNA的表达情况。结果：翼状胬肉组织上皮层均可见不同程度的增生,上皮下组织成纤维细胞增生活跃、新生血管增多,血管周围有炎性细胞浸润。VEGF在翼状胬肉组织上皮细胞胞浆及血管内皮呈棕黄色染色；PCNA在翼状胬肉组织上皮细胞胞核呈浅黄色或深棕色染色；翼状胬肉组织VEGF和PCNA的阳性表达率是88.5%,正常结膜组织中VEGF和PCNA的阳性表达率分别20%、40%；VEGF在翼状胬肉和正常结膜中的表达差异存在统计学意义(U=29.000,W=84.000,Z=-4.205,P=0.000)；PCNA在翼状胬肉和正常结膜中的表达差异存在统计学意义(U=74.000,W=129.000,Z=-2.868,P=0.004)。VEGF与PCNA在翼状胬肉组织中表达的相关系数rs=0.599,P=0.000。结论：翼状胬肉组织病理学表现以增生性病变为主,翼状胬肉组织中VEGF和PCNA的表达增加,VEGF和PCNA在翼状胬肉组织的表达呈正相关,研究结果提示VEGF和PCNA可能共同参与了翼状胬肉的发病过程；
[Abstract]:Aim: to investigate the expression of vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA) in pterygium and normal conjunctiva and the correlation between VEGF,PCNA and VEGF,PCNA. Materials and methods: 35 cases of pterygium and 10 cases of normal conjunctiva were selected from the first affiliated Hospital of Jinan University from August 2008 to April 2010. Histopathological examination and VEGF,PCNA immunohistochemical examination were performed by routine HE staining. Histopathological findings and expression of VEGF,PCNA were observed respectively. Results: in pterygium, the epithelium of pterygium showed various degrees of proliferation, fibroblast proliferation of subcutaneous tissue was active, and neovascularization was increased. There were inflammatory cells infiltrating around the blood vessels. VEGF was stained in the cytoplasm of pterygium epithelium and vascular endothelium. PCNA was stained light yellow or dark brown in the nucleus of pterygium epithelial cells. The positive expression rate of VEGF and PCNA in pterygium tissue was 88.5.The positive expression rate of VEGF and PCNA in normal conjunctiva tissue was 20 and 40, respectively. There was significant difference between pterygium and normal conjunctiva. There was significant difference in the expression of PCNA between pterygium and normal conjunctiva (UH74.000). The correlation coefficient between PCNA and PCNA expression in pterygium tissue was rs=0.599,P=0.000.. Conclusion: the histopathological manifestations of pterygium are mainly proliferative lesions. The expression of VEGF and PCNA in pterygium is positively correlated with the expression of PCNA in pterygium. The results suggest that VEGF and PCNA may be involved in the pathogenesis of pterygium.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R777.33

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