Toll样受体2和4亚型在大鼠变应性鼻炎发病中的作用

发布时间：2018-09-14 10:14

【摘要】：目的:应用卵清蛋白(OVA)建立大鼠变应性鼻炎(AR)模型,探讨Toll样受体(TLR)2和4亚型(TLR2和TLR4)在AR发病中调节非特异性免疫和特异性免疫的作用。方法:80只大鼠随机分为正常组、AR模型组、AR+脂多糖(LPS)组和AR+肽聚糖(PGN)组,每组20只。HE染色观察各组大鼠鼻黏膜组织形态学改变并计数炎症细胞浸润数,免疫组织化学法检测各组大鼠鼻黏膜组织中TLR2、TLR4和IgE蛋白表达水平,Real-time PCR法检测各组大鼠鼻黏膜组织中TLR2和TLR4mRNA表达水平。结果:与正常组比较,模型组、AR+LPS组和AR+PGN组大鼠鼻部症状评分和炎症细胞计数明显升高(P0.05)。与正常组比较,模型组大鼠鼻黏膜组织变应性损伤明显,鼻黏膜以嗜酸性粒细胞浸润为主,AR+LPS组和AR+PGN组大鼠鼻黏膜组织以中性粒细胞浸润为主。与正常组比较,模型组、AR+LPS组和AR+PGN组大鼠鼻黏膜组织中TLR2、TLR4和IgE表达水平明显增高(P0.05);与模型组比较,AR+LPS组大鼠鼻黏膜组织中TLR4和IgE表达水平明显升高(P0.05);AR+PGN组大鼠鼻粘膜组织中TLR2和IgE的表达水平较模型组也明显升高(P0.05)。结论:应用OVA腹腔注射及局部喷鼻能有效建立AR模型,TLR2和TLR4在AR发病中发挥调节非特异性免疫与特异性免疫的作用。
[Abstract]:AIM: To establish a rat model of allergic rhinitis (AR) by ovalbumin (OVA) and investigate the effects of Toll-like receptor (TLR) 2 and 4 subtypes (TLR2 and TLR4) on regulating non-specific and specific immunity in AR. Methods: 80 rats were randomly divided into normal group, AR model group, AR + lipopolysaccharide (LPS) group and AR + peptidoglycan (PGN) group, with 20 rats in each group. The expression of TLR2, TLR4 and IgE protein in nasal mucosa of rats in each group was detected by immunohistochemistry, and the expression of TLR2 and TLR4 mRNA in nasal mucosa of rats in each group was detected by Real-time PCR. Compared with the normal group, the allergic lesion of the nasal mucosa was obvious in the model group, and eosinophil infiltration was the main infiltration in the nasal mucosa. The infiltration of neutrophils was the main infiltration in the AR + LPS group and the AR + PGN group. The expression levels of TLR2, TLR4 and IgE in nasal mucosa of AR+PGN group were significantly higher than those of model group (P 0.05); the expression levels of TLR4 and IgE in nasal mucosa of AR+LPS group were significantly higher than those of AR+PGN group (P 0.05); the expression levels of TLR2 and IgE in nasal mucosa of AR+PGN group were also significantly higher than those of model group (P 0.05). TLR2 and TLR4 play an important role in regulating nonspecific and specific immunity in AR.
【作者单位】： 莆田学院基础医学院生理学教研室;福建医科大学附属第一医院眼科;
【基金】：国家自然科学基金资助课题(81270976) 福建省教育厅科研基金资助课题(JK2013045) 福建省莆田市科技局科研基金资助课题(2010S06) 莆田学院育苗基金资助课题(2014058)
【分类号】：R765.21

【参考文献】

相关期刊论文 前4条

1 宋建明;黄秀清;林燕惠;张敏;;变应性鼻炎大鼠模型的建立[J];吉林大学学报(医学版);2012年06期

2 彭建亮;冯凯;路浩军;张军;郑燕华;景青萍;;人Toll样受体4基因5'远端增强子样区定位及反式作用因子鉴定[J];解放军医学杂志;2012年05期

3 吕社民;孟列素;朱文华;;Toll样受体参与类风湿性关节炎发病机制的研究进展[J];西安交通大学学报(医学版);2014年04期

4 田雷;周东生;孙水;张晨;樊立宏;王坤正;;Toll样受体4信号通路过度激活在大鼠激素性股骨头坏死中的作用[J];西安交通大学学报(医学版);2014年05期

【共引文献】

相关期刊论文 前7条

1 苏磊;耿曼英;张俊星;张慧;孟令浩;;盐酸赛洛唑啉联合鼻用糖皮质激素对变应性鼻炎模型大鼠鼻黏膜组织中ICAM-1、IL-12表达的影响[J];郑州大学学报(医学版);2014年03期

2 张敏;刘黎星;徐丽;杨海东;朱志红;;Toll样受体核因子通路在变应性鼻炎发病中的作用[J];兰州大学学报(医学版);2014年02期

3 周俊雄;尹佳;崔乐;姜楠楠;席广鹏;马仕坤;孔瑞;徐迎阳;刁颖;吴锴;;中国变态反应学2012年度科技发展荟萃报告[J];中华临床免疫和变态反应杂志;2013年03期

4 张清照;]嬏飳粗，

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