胆红素对离体培养大鼠耳蜗基底膜的药理效应观察

发布时间：2018-10-17 14:05

【摘要】：目的观察胆红素对离体培养的新生SD大鼠耳蜗器官的损伤效应。方法健康新生3日龄SD大鼠18只,雌雄不限,分离其双侧的内耳共得到耳蜗器官组织36个,体外培养,12h后换液。选择贴壁、存活良好的组织30个入选实验,并随机分为两组(15个/组):实验组于贴壁第二天开始进行胆红素处理,对照组加入相应体积的生理盐水。处理后24h,实验组和对照组同时终止培养,并对其进行固定、免疫组织化学染色,共聚焦显微镜观察毛细胞及螺旋神经节神经元的形态变化并计数,统计对比。结果胆红素组离体培养的耳蜗器官中螺旋神经节神经元胞体及其树突纤维与对照组相比有明显的减少,内外毛细胞形态完整,数量无明显变化。结论大鼠耳蜗器官螺旋神经节神经元对胆红素的毒性敏感,而内外毛细胞对其毒性相对不敏感。
[Abstract]:Objective to observe the damage effect of bilirubin on cochlear organ of neonatal SD rats in vitro. Methods A total of 36 cochlear organs were isolated from 18 healthy 3-day-old SD rats, male and female. Cochlear organs were isolated from both sides of the inner ear and cultured in vitro. After 12 hours of culture, the cochlear organs were changed. Thirty tissues that adhered to the wall and survived well were selected and randomly divided into two groups (15 / group): the experimental group was treated with bilirubin on the second day of adhesion and the control group was added with the corresponding volume of normal saline. 24 hours after treatment, both the experimental group and the control group were cultured at the same time and fixed with immunohistochemical staining. The morphological changes of hair cells and spiral ganglion neurons were observed by confocal microscope and counted and compared statistically. Results the cell bodies and dendritic fibers of spiral ganglion neurons in the cochlear organs of bilirubin group were significantly decreased compared with those of the control group. The morphology of the inner and outer hair cells in the bilirubin group was intact and the number of the spiral ganglion neurons had no obvious change. Conclusion Spiral ganglion neurons of rat cochlea organ are sensitive to the toxicity of bilirubin, but the inner and outer hair cells are relatively insensitive to the toxicity of bilirubin.
【作者单位】：福建医科大学附属第一医院耳鼻咽喉头颈外科;解放军总医院耳鼻咽喉头颈外科解放军耳鼻咽喉科研究所;
【基金】：国家自然科学基金面上项目(81271082) 国家重大科学研究计划(2012CB967900)资助
【分类号】：R764.35

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