XIAP与Apaf-1在鼻内翻性乳头状瘤中的表达及意义的研究
发布时间：2018-10-20 19:21
【摘要】：目的:本实验主要从鼻内翻性乳头状瘤(nasal inverted papilloma,NIP)的病理形态出发,根据NIP的病理特点及细胞凋亡与肿瘤发生的关系,采用免疫组织化学方法检测细胞凋亡相关因子X-连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis pretein,XIAP)及凋亡蛋白酶活化因子-1(apoptptic protease activating factor-1,Apaf-1)在NIP中的表达情况并分析其相互关系。探讨细胞凋亡机制在NIP发生发展中的作用,为NIP的早期临床诊断及疾病的发展程度提供新的参考依据,为NIP的治疗也提供了新的指导依据。 方法:采用兔抗人XIAP多克隆抗体和兔抗人Apaf-1多克隆抗体,通过免疫组织化学技术检测25例NIP组织标本中XIAP及Apaf-1的表达;同时以鼻鳞状细胞癌(nasal squamous cell carcinoma, NSCC)及正常下鼻甲(inferior turbinate,IT)组织为对照组,检测21例NSCC存档蜡块标本及18例IT标本中XIAP及Apaf-1的表达,实验数据采用SPSS17.0软件包X~2检验及Spearman相关分析等。 结果:(1)XIAP在IT、NIP、NSCC中的表达阳性率分别是16.67%(3/18)、60.00%(15/25)、90.48%(19/21),两两相比,差异均有显著性(P0.05)。(2)Apaf-1在IT、NIP、NSCC中的表达阳性率分别是94.44%(17/18)、64.00%(16/25)、23.81%(5/21),两两相比,其差异均有显著性(P0.05)。(3)在15例XIAP阳性表达的NIP中Apaf-1阳性表达例数为13例,在Apaf-1阳性表达的16例NIP病例中XIAP阳性及阴性例数分别是13例和3例,两者相比差异具有显著性(X~2=8.362,P0.05),据Spearman统计分析结果Rs=-0.895,可知NIP中XIAP与Apaf-1的表达呈负相关。 结论:XIAP及Apaf-1在NIP中表达介于IT与NSCC之间,表达率分别呈递增及递减趋势,两两相比均具有显著差异(P0.05),提示XIAP的升高与Apaf-1的降低与肿瘤的发生发展有密切关系。XIAP与Apaf-1在NIP的表达具有负相关性,且在不同的NIP病例中二者的表达程度不同,提示XIAP异常高表达与Apaf-1明显低表达的病例可能更易恶性变或复发。通过对二者的检测可以对NIP的早期发现,发展及恶性变等生物学行为提供依据,从而为有效治疗NIP,选用合理的手术方式提供理论依据。
[Abstract]:Objective: this study was based on the pathological morphology of nasal inverted papilloma (nasal inverted papilloma,NIP), according to the pathological characteristics of NIP and the relationship between apoptosis and tumorigenesis. The expression of apoptosis-related factor X- linkage inhibitor of apoptosis protein (X-linked inhibitor of apoptosis pretein,XIAP) and apoptotic protease activator 1 (apoptptic protease activating factor-1,Apaf-1 in NIP was detected by immunohistochemical method and the relationship between them was analyzed. To explore the role of apoptosis mechanism in the pathogenesis and development of NIP, to provide a new reference for the early clinical diagnosis and disease development of NIP, as well as a new guiding basis for the treatment of NIP. Methods: using rabbit anti-human XIAP polyclonal antibody and rabbit anti-human Apaf-1 polyclonal antibody, the expression of XIAP and Apaf-1 in 25 cases of NIP were detected by immunohistochemistry. The expression of XIAP and Apaf-1 in 21 cases of NSCC archived wax and 18 cases of IT were detected with (nasal squamous cell carcinoma, NSCC) and normal inferior turbinate (inferior turbinate,IT) as control group. The data were detected by SPSS17.0 software X2 and Spearman correlation analysis. Results: (1) the positive rates of XIAP expression in IT,NIP,NSCC were 16.67% (3 / 18), 60.00% (15 / 25), 90.48% (19 / 21), respectively (P0.05). (2). The positive rates of Apaf-1 in IT,NIP,NSCC were 94.44% (17 / 18), 64.00% (16 / 25), 23.81% (5 / 21), respectively. There were significant differences between them (P0.05). (3). The number of Apaf-1 positive expression in NIP of 15 XIAP positive cases was 13, and the XIAP positive and negative in Apaf-1 positive NIP was 13 and 3, respectively. There was significant difference between the two groups (P 0.05). According to Spearman statistical analysis, the expression of XIAP and Apaf-1 in NIP was negatively correlated with that of XIAP. Conclusion: the expression of XIAP and Apaf-1 in NIP is between IT and NSCC, and the expression rate is increasing and decreasing, respectively. There was significant difference between the two groups (P0.05), which suggested that the increase of XIAP and the decrease of Apaf-1 were closely related to the occurrence and development of tumor. The expression of XIAP and Apaf-1 in NIP was negatively correlated, and the expression degree of XIAP and Apaf-1 in different NIP cases was different. The results suggest that the cases with abnormal high expression of XIAP and obvious low expression of Apaf-1 may be more prone to malignant change or recurrence. The detection of both can provide the basis for the early detection, development and malignant change of NIP, so as to provide theoretical basis for the effective treatment of NIP, with reasonable surgical methods.
【学位授予单位】：佳木斯大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.62
[Abstract]:Objective: this study was based on the pathological morphology of nasal inverted papilloma (nasal inverted papilloma,NIP), according to the pathological characteristics of NIP and the relationship between apoptosis and tumorigenesis. The expression of apoptosis-related factor X- linkage inhibitor of apoptosis protein (X-linked inhibitor of apoptosis pretein,XIAP) and apoptotic protease activator 1 (apoptptic protease activating factor-1,Apaf-1 in NIP was detected by immunohistochemical method and the relationship between them was analyzed. To explore the role of apoptosis mechanism in the pathogenesis and development of NIP, to provide a new reference for the early clinical diagnosis and disease development of NIP, as well as a new guiding basis for the treatment of NIP. Methods: using rabbit anti-human XIAP polyclonal antibody and rabbit anti-human Apaf-1 polyclonal antibody, the expression of XIAP and Apaf-1 in 25 cases of NIP were detected by immunohistochemistry. The expression of XIAP and Apaf-1 in 21 cases of NSCC archived wax and 18 cases of IT were detected with (nasal squamous cell carcinoma, NSCC) and normal inferior turbinate (inferior turbinate,IT) as control group. The data were detected by SPSS17.0 software X2 and Spearman correlation analysis. Results: (1) the positive rates of XIAP expression in IT,NIP,NSCC were 16.67% (3 / 18), 60.00% (15 / 25), 90.48% (19 / 21), respectively (P0.05). (2). The positive rates of Apaf-1 in IT,NIP,NSCC were 94.44% (17 / 18), 64.00% (16 / 25), 23.81% (5 / 21), respectively. There were significant differences between them (P0.05). (3). The number of Apaf-1 positive expression in NIP of 15 XIAP positive cases was 13, and the XIAP positive and negative in Apaf-1 positive NIP was 13 and 3, respectively. There was significant difference between the two groups (P 0.05). According to Spearman statistical analysis, the expression of XIAP and Apaf-1 in NIP was negatively correlated with that of XIAP. Conclusion: the expression of XIAP and Apaf-1 in NIP is between IT and NSCC, and the expression rate is increasing and decreasing, respectively. There was significant difference between the two groups (P0.05), which suggested that the increase of XIAP and the decrease of Apaf-1 were closely related to the occurrence and development of tumor. The expression of XIAP and Apaf-1 in NIP was negatively correlated, and the expression degree of XIAP and Apaf-1 in different NIP cases was different. The results suggest that the cases with abnormal high expression of XIAP and obvious low expression of Apaf-1 may be more prone to malignant change or recurrence. The detection of both can provide the basis for the early detection, development and malignant change of NIP, so as to provide theoretical basis for the effective treatment of NIP, with reasonable surgical methods.
【学位授予单位】：佳木斯大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.62
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