c-jun与brg1在喉鳞癌中的表达及临床意义

发布时间：2018-10-21 13:12

【摘要】：目的:观察c-jun、brg1在喉鳞癌的表达及意义,并分析二者与喉鳞癌的临床病理各因素的关系,探讨c-jun、brg1两者在喉鳞癌患者中表达的相关性,为喉鳞癌的早期诊断提供可供参考的依据。方法:用免疫组织化学染色法检测45例喉鳞癌组织、10例癌旁正常组织、10例声带息肉中c-jun、brg1表达,比较喉鳞癌组织、癌旁正常组织及声带息肉中c-jun、brg1表达的差异性。观察喉鳞癌组织c-jun、brg-1表达与临床病理各因素:年龄、性别、病理分级、临床分期、淋巴结转移、原发部位等之间的相互关系。结果: 1、c-jun在喉鳞癌中的阳性表达率明显高于癌旁组织及声带息肉。c-jun在喉鳞癌与癌旁组织、喉鳞癌与声带息肉中的阳性表达率比较,有显著性差异(P0.05),而在癌旁组织与声带息肉中的阳性表达率比较,无差异性(P0.05)。 c-jun在喉鳞癌患者中,发现年龄、性别及原发部位组间中阳性表达率比较,无统计学意义(p0.05),而在病理分级,临床分期及有无淋巴结转移组间中阳性表达率有统计学意义(p0.05)。c-jun在高、中及低分化喉鳞癌中的阳性率分别为89.5%,80.0%及54.5%,其阳性表达率随喉鳞癌病理分级的降低而逐渐下降,且高、中分化,中、低分化组间比较,无统计学意义(p0.05),高、低分化组间比较差异有显著性(p0.05)。 2、brg1在癌旁组织中的阳性表达率明显高于喉鳞癌和声带息肉。brg1在喉鳞癌与癌旁组织,癌旁组织与声带息肉中的阳性表达率比较,有显著性差异(P0.05),在喉鳞癌与声带息肉中阳性表达率比较,无统计学意义(P0.05)。 brg1在喉鳞癌患者中,发现年龄、性别及原发部位组间中阳性表达率比较,无统计学意义(p0.05),而在病理分级,临床分期及有无淋巴结转移组间中阳性表达率有统计学意义(p0.05)。brg1在高、中及低分化喉鳞癌中的阳性率分别为73.7%,40.0%及18.2%,其阳性表达率随喉鳞癌病理分级的降低而逐渐下降,且中、低分化组间比较,无统计学意义(p0.05),高、中分化,高、低分化组间比较差异有显著性(p0.05)。 3、c-jun、brg1两种蛋白在喉鳞癌中的表达无相关性r=0.5874(p0.05)。结论: 1、c-jun的表达阳性率在喉鳞癌组织显著高于癌旁组织和声带息肉,而brg1的表达阳性率在癌旁组织中明显高于喉鳞癌及声带息肉,提示二者与喉鳞癌的发生、发展密切相关。 2、c-jun和brg1的表达与喉鳞癌的病理分级、临床分期、淋巴结的有无转移相关,而与年龄、性别及原发部位无关,c-jun可能在喉鳞癌的发生、发展中起促进作用,而brg1正好相反,c-jun及brg1的表达可能提示组织的恶性程度。 3、c-jun和brg1在喉鳞癌中表达呈无相关性,提示二者可能在多种因素介导下参与喉鳞癌的发生及发展。
[Abstract]:Objective: to observe the expression and significance of c-junn brg1 in laryngeal squamous cell carcinoma (LSCC), and to analyze the relationship between c-junbbrg1 and clinicopathologic factors of laryngeal squamous cell carcinoma (LSCC), and to explore the correlation between c-junnbr g1 and c-junbbrg1 expression in laryngeal squamous cell carcinoma (LSCC) so as to provide reference for early diagnosis of laryngeal squamous cell carcinoma (SCC). Methods: the expression of c-junn brg1 in 45 cases of laryngeal squamous cell carcinoma (LSCC), 10 cases of adjacent normal tissues and 10 cases of vocal cord polyps were detected by immunohistochemical staining. The difference of c-junbbrg1 expression in laryngeal squamous cell carcinoma (LSCC), adjacent normal tissues and vocal cord polyps was compared. To observe the relationship between c-junn brg-1 expression and clinicopathological factors: age, sex, pathological grade, clinical stage, lymph node metastasis and primary location of laryngeal squamous cell carcinoma (LSCC). Results: 1 the positive expression rate of c-jun in laryngeal squamous cell carcinoma was significantly higher than that in paracancerous tissue and vocal cord polyp. The positive expression rate of c-jun in laryngeal squamous cell carcinoma and paracancerous tissue, laryngeal squamous cell carcinoma and vocal cord polyp was higher than that in laryngeal squamous cell carcinoma and vocal cord polyp. There was significant difference (P0.05), but there was no difference between the expression rate of c-jun in adjacent tissues and vocal cord polyps (P0.05). The positive expression rate of c-jun in laryngeal squamous cell carcinoma patients was found in age, sex and primary location group. There was no statistical significance (p0. 05), but the positive expression rate in pathological grade, clinical stage and lymph node metastasis group was statistically significant (p0. 05). The expression rate of c-jun was higher in the group with or without lymph node metastasis (p0. 05). The positive rates of middle and low differentiation laryngeal squamous cell carcinoma were 80.0% and 54.5%, respectively. The positive expression rate decreased gradually with the decrease of pathological grade of laryngeal squamous cell carcinoma. There was significant difference between the low differentiation groups (p0. 05). 2the positive expression rate of BRg1 in adjacent tissues was significantly higher than that in laryngeal squamous cell carcinoma and vocal cord polyp. The positive expression rate of brg1 in laryngeal squamous cell carcinoma and paracancerous tissue, paracancerous tissue and vocal cord polyp was higher than that in laryngeal squamous cell carcinoma and vocal cord polyp. There was significant difference (P0.05). There was no significant difference between the positive expression rate of brg1 in laryngeal squamous cell carcinoma and vocal cord polyp (P0.05). There was no statistical significance (p0. 05), but the positive expression rate in pathological grade, clinical stage and lymph node metastasis group was statistically significant (p0. 05). The expression rate of brg1 was higher in the group with or without lymph node metastasis (p0. 05). The positive rates of middle and low differentiated laryngeal squamous cell carcinoma were 40.0% and 18.2%, respectively. The positive expression rate decreased gradually with the decrease of pathological grade of laryngeal squamous cell carcinoma, and there was no significant difference between middle and low differentiation groups (p0.05). The expression of c-junbbrg1 protein in laryngeal squamous cell carcinoma (LSCC) was not correlated with r-0.5874 (p0.05). Conclusion: 1 the positive rate of c-jun expression in laryngeal squamous cell carcinoma is significantly higher than that in adjacent laryngeal squamous cell carcinoma and vocal cord polyp, while the positive rate of brg1 in adjacent laryngeal squamous cell carcinoma and vocal cord polyp is significantly higher than that in adjacent laryngeal squamous cell carcinoma and vocal cord polyp, suggesting that the expression of c-jun in laryngeal squamous cell carcinoma is associated with laryngeal squamous cell carcinoma. 2the expression of c-jun and brg1 was correlated with the pathological grade, clinical stage and lymph node metastasis of laryngeal squamous cell carcinoma, but not with age, sex and primary location. C-jun may occur in laryngeal squamous cell carcinoma. The expression of c-jun and brg1 may indicate the degree of malignancy of tissue. 3The expression of c-jun and brg1 in laryngeal squamous cell carcinoma has no correlation. These results suggest that they may be involved in the occurrence and development of laryngeal squamous cell carcinoma (LSCC).
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.65

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