下咽鳞癌中uPA的表达和微血管密度与肿瘤侵袭转移的关系

发布时间：2018-10-21 18:52

【摘要】：目的检测尿激酶纤溶酶原激活剂(uPA)在下咽鳞状细胞癌组织及癌旁正常黏膜中的表达以及CD34抗体标志的微血管密度(MVD)的差异,探讨其与下咽癌侵袭转移的关系。方法应用免疫组织化学SP法检测60例下咽癌组织及20例下咽部癌旁正常黏膜组织中uPA蛋白的表达及以CD34抗体标志的微血管密度。结果 uPA蛋白在下咽鳞癌组织中的表达率为78.33%(47/60),明显高于癌旁正常黏膜组织中的表达(P0.001),uPA蛋白的表达与其临床分期、病理分化程度及淋巴结转移呈显著相关关系(P0.05);MVD计数与肿瘤的临床分期、病理分化程度及淋巴结转移呈显著相关关系(P0.05或0.01);两者与患者发病年龄及肿瘤原发解剖分区均无明显相关性;下咽癌组织中MVD计数与uPA的阳性表达呈正相关关系(r=0.561,P0.01);uPA阳性并MVD高表达组的淋巴结转移率为90.91%(20/22),明显高于uPA阳性并MVD低表达组(52.00%)及uPA阴性MVD低表达组(30.77%)(2χ=9.538,P0.05)。结论 uPA蛋白的高表达促进了肿瘤新生血管的生成,活跃的新生血管生成和uPA蛋白的高表达与下咽癌的侵袭及颈部淋巴结转移密切相关,uPA高表达且MVD计数高的下咽癌患者发生肿瘤浸润及颈淋巴结转移的风险明显增高。uPA的表达强度及MVD的计数值可作为判断下咽鳞状细胞癌生长、浸润及转移能力的重要指标。
[Abstract]:Objective to investigate the expression of urokinase plasminogen activator (uPA) in hypopharyngeal squamous cell carcinoma and its adjacent normal mucosa and the difference of microvessel density (MVD) of CD34 antibody marker, and to explore the relationship between the expression of urokinase plasminogen activator (uPA) and invasion and metastasis of hypopharyngeal carcinoma. Methods Immunohistochemical SP method was used to detect the expression of uPA protein and microvessel density (MVD) marked by CD34 antibody in 60 cases of hypopharyngeal carcinoma and 20 cases of normal mucosa adjacent to hypopharyngeal carcinoma. Results the expression rate of uPA protein in hypopharyngeal squamous cell carcinoma was 78.33% (47 / 60), which was significantly higher than that in adjacent normal mucosa (P0.001), uPA protein expression and its clinical stage). There was a significant correlation between pathological differentiation and lymph node metastasis (P0.05); MVD count and clinical stage of the tumor. There was no significant correlation between pathological differentiation and lymph node metastasis (P0.05 or 0.01). There was a positive correlation between the MVD count and the positive expression of uPA in hypopharyngeal carcinoma tissues. The lymph node metastasis rate was 90.91% (20 / 22) in the group of positive); uPA and high MVD expression, which was significantly higher than that in the group of uPA positive and low MVD expression (52.00%) and the low expression of uPA MVD (30.77%) (蠂 2 = 9.538P 0.05). Conclusion the overexpression of uPA protein promotes the angiogenesis of tumor. Active neovascularization and high expression of uPA protein were closely related to the invasion of hypopharyngeal carcinoma and cervical lymph node metastasis. The risk of tumor invasion and cervical lymph node metastasis in hypopharyngeal carcinoma patients with high uPA expression and high MVD count was significantly increased. The expression intensity of uPA and the count value of MVD can be used to judge the growth of hypopharyngeal squamous cell carcinoma. An important indicator of infiltration and metastasis.
【作者单位】：山东大学齐鲁医院耳鼻咽喉-头颈外科;
【分类号】：R739.63

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