乙醇激活的鼻咽癌细胞氯电流的分子机制

发布时间：2018-10-26 12:14

【摘要】：目的:研究乙醇对低分化鼻咽癌CNE-2Z细胞氯通道的影响并探讨其分子机制。方法:MTT检测乙醇对细胞生长的影响;全细胞膜片钳技术记录乙醇对氯电流的影响;应用氯通道阻断剂分析该电流的特性;siRNA干扰技术分析乙醇敏感氯通道的分子本质。结果:在等渗灌流液中记录到微弱的背景氯电流,随灌流液中乙醇浓度(0.17~170 mmol/L)的升高,所激活的氯电流呈倒U型曲线状,17 mmol/L的乙醇激活电流达到峰值,电流呈较明显的外向优势,能被高渗液及氯通道阻断剂5-nitro-2-(3-phenylpropylamino)benzoic acid(NPPB)所阻断;干扰ClC-3氯通道基因表达后乙醇激活的氯电流明显减小。结论:乙醇可能通过激活CNE-2Z细胞的CIC-3氯通道,诱导氯电流的产生。
[Abstract]:Aim: to investigate the effect of ethanol on chloride channels in poorly differentiated nasopharyngeal carcinoma (NPC) CNE-2Z cells and its molecular mechanism. Methods: MTT was used to detect the effect of ethanol on cell growth; whole-cell patch clamp technique was used to record the effect of ethanol on chloride current; chlorine channel blocker was used to analyze the characteristics of the current; and siRNA interference technique was used to analyze the molecular nature of ethanol-sensitive chloride channel. Results: the weak background chlorine current was recorded in the perfusion solution. With the increase of ethanol concentration (0.17 ~ 170 mmol/L), the activated chloride current was inversely U-shaped, and the peak value of the ethanol activation current was reached at 17 mmol/L. The current showed obvious outward advantage and could be blocked by high percolation and 5-nitro-2- (3-phenylpropylamino) benzoic acid (NPPB). The chloride current activated by ethanol was significantly decreased after interfering with the expression of ClC-3 chloride channel gene. Conclusion: ethanol may induce the generation of chloride currents by activating CIC-3 chloride channels in CNE-2Z cells.
【作者单位】：暨南大学医学院生理学系;暨南大学医学院药理学系;
【基金】：国家自然科学基金资助项目(No.81272223) 广州市科技计划(No.2013J500015) 教育部博士点基金资助项目(No.20124401110009) 东莞市科技计划(No.2011108102006)
【分类号】：R739.63

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