Ezrin和RhoA蛋白在鼻咽癌组织中的表达及临床意义

发布时间：2018-11-03 12:41

【摘要】： 目的：检测Ezrin和RhoA蛋白各自在不同临床分期鼻咽癌(Nasopharyngeal Carcinoma, NPC)组织中的表达,探讨二者在NPC发生和发展中的作用以及它们在其中的相互关系。为NPC浸润转移的机制提供新的理论依据。为NPC的早期诊断、临床防治及预后判断提供新的有价值的指标。 方法： 1.选取有完整临床资料及随访资料的150例NPC病例石蜡包埋组织标本作为实验组,选取20例鼻咽粘膜慢性炎石蜡包埋组织作为对照。 2.应用免疫组化Supervision二步法分别检测NPC和对照组组织中Ezrin和RhoA蛋白的表达情况。 3.采用SPSS13.0统计软件进行数据处理。用x2检验、Spearman等级相关分析法分析Ezrin和RhoA蛋白表达与NPC患者临床病理特征之间的关系；用Kaplan-Meier法进行单因素生存分析,log-rank法进行样本间生存率曲线差异检验。 结果： 1. Ezrin与RhoA蛋白在NPC组织中的阳性表达率分别为74.7%和62.7%,在对照组组织中基本不表达。两组差异都有非常显著统计学意义(均为PO.001)。 2. Ezrin与RhoA蛋白阳性表达率在NPC临床TNM分期的T1、T2、T3、T4分别为：29.4%、65.5%、92.3%、88.5%和17.6%、52.7%、78.8%、80.8%,除T3与T4期,各T分期之间比较均有显著或非常显著统计学意义(P0.05或P0.001)；在N0、N1、N2、N3分别为53.8%、71.4%、79.6%、87.9%和46.2%、54.8%、65.3%、81.8%,Ezrin蛋白表达在N0与N2,N0与N3分期之间有显著差异(P0.05),RhoA蛋白表达在N0与N1,N0与N3以及N1与N3分期之间有显著差异(P0.05)；在M0与M1分别为72.5%、94.7%和60.3%、84.2%, M0与M1之间比较均有显著统计学意义(均为P0.05)。 3. Ezrin与RhoA蛋白阳性表达率在临床分期Ⅰ、Ⅱ、Ⅲ、Ⅳ期中分别为50%、40%、80.7%、86.9%和0%、26.7%、64.9%、80.3%,两指标各期之间均有非常显著统计学意义(均为P0.001)。 4.NPC组织中Ezrin与RhoA两者表达呈显著正相关(Rs=0.486,P0.001)。 5. Ezrin和RhoA蛋白表达阳性的患者,治疗后1年、3年及5年生存率分别为：75%,55%,33%和84%,60%,33%,均低于阴性表达100%,92%,78%和93%,71%,63%。均有显著性差异(x2=12.943,P=0.000及x2=9.869,P=0.0020.05)。 结论： 1. Ezrin和RhoA蛋白表达均与NPC的发生可能有关,但更可能是Ezrin和RhoA蛋白表达的NPC细胞本身具有浸润转移潜能。 2. Ezrin和RhoA蛋白表达均参与了NPC的早期局部浸润转移、淋巴结转移和远处器官转移等过程,并很有可能是重要的参与因子。 3. Ezrin与RhoA蛋白表达呈显著正相关,进一步间接证明了两者在结构、功能上和基因表达等方面有着密切联系,同时表明它们很可能为NPC浸润转移的共同或协同促进因子。 4. Ezrin和RhoA与NPC的预后有关。Ezrin或RhoA阳性表达的NPC患者平均生存率低,特别是Ezrin和RhoA同时阳性表达者较Ezrin和RhoA同时阴性表达者生存期短,预后差。
[Abstract]:Aim: to detect the expression of Ezrin and RhoA proteins in (Nasopharyngeal Carcinoma, NPC) tissues of nasopharyngeal carcinoma at different clinical stages and to explore their roles in the pathogenesis and development of NPC and their relationship. It provides a new theoretical basis for the mechanism of NPC invasion and metastasis. To provide a new valuable index for early diagnosis, clinical prevention and prognosis of NPC. Methods: 1. Paraffin embedded tissue specimens of 150 cases of NPC with complete clinical data and follow-up data were selected as experimental group and 20 cases of nasopharyngeal mucosa chronic inflammation paraffin embedded tissue as control group. 2. The expression of Ezrin and RhoA in NPC and control group was detected by immunohistochemical Supervision two-step method. 3. SPSS13.0 statistical software is used for data processing. The relationship between the expression of Ezrin and RhoA protein and the clinicopathological characteristics of NPC patients was analyzed by x2 test and Spearman rank correlation analysis. Results: 1. The positive expression rates of Ezrin and RhoA in NPC were 74.7% and 62.7%, respectively. The differences between the two groups were statistically significant (both PO.001). 2. The positive expression rate of Ezrin and RhoA protein in clinical TNM stage of NPC was 29.4% and 65.5%, respectively. The positive expression rate of Ezrin and RhoA protein was 88.8% and 52.7%, respectively, except T 3 and T 4, respectively, with the exception of T 3 and T 4, and T 3, T 4, T 3 and T 4, respectively. There were significant or very significant statistical differences among T stages (P0.05 or P0.001). At N0, N1, N2N3 was 53.81.41, and 79.6% and 46.2%, 54.8and 65.3%, respectively, and the Ezrin protein was expressed in N0 and N2, and the Ezrin protein was expressed in N0 and N2. There was significant difference between N0 and N3 stages (P0.05). The expression of), RhoA protein was significantly different between N0 and N1N0 and N3 and N1 and N3 stages (P0.05). M0 and M1 were 72.5% and 60.3%, respectively, and there were significant differences between M0 and M1 (P0.05). 3. The positive expression rates of Ezrin and RhoA protein in clinical stages 鈪，

本文编号：2307823