人葡萄膜黑色素瘤小鼠肝内种植模型生长行为研究

发布时间：2018-11-04 08:52

【摘要】：目的:采用肝内注射人眼葡萄膜恶性黑色素瘤株(MUM2B细胞)方法制备该肿瘤的小鼠肝内种植模型,研究肿瘤在其体内的生物学行为。方法:选用BALB/C-nu裸鼠,将5×104个MUM2B细胞注入裸鼠肝脏包膜下。连续观察6周,每周随机处死3只裸鼠以观察成瘤率及侵袭率。采集裸鼠的荷瘤肝脏、心脏、脾脏、肺脏、肾脏、胃和肠等组织器官,常规石蜡切片,HE染色后镜检肿瘤在肝脏内的生长行为及其向其他脏器侵袭和转移的情况。结果:该模型的成瘤率可达83.3%。接种后的前4周,肿瘤在肝脏内浸润性生长,未侵犯周围脏器;接种后第5周,肿瘤开始侵袭肾脏、脾脏、胃和十二指肠的包膜,并可突破胃及肠道的包膜,在其浆膜层中侵袭生长;接种后第6周,肿瘤对肾脏、脾脏、十二指肠和胃的侵袭加剧。肿瘤细胞可侵入裸鼠十二指肠的肌层内生长,亦可侵入裸鼠胃肌层及胃黏膜下层,甚至向胃黏膜肌层内侵袭生长;接种后第6周,肿瘤开始向裸鼠肺脏转移,所有受检裸鼠的肺门组织及肺实质内均可查见转移性瘤灶,转移率达100%;除肺脏外,其余脏器内均未查见转移性瘤灶,提示肺脏是该肝内种植瘤的主要转移靶器官。此外,荷瘤2周后,裸鼠脾脏红髓增生明显,髓内可见大量巨核细胞及髓系来源的粒细胞,呈现白血病样反应;荷瘤4周后,脾脏内星空样现象明显,巨噬细胞活跃,提示裸鼠脾脏组织学改变与荷瘤生长状态密切相关。结论:裸鼠肝内注射MUM2B细胞能够建立起稳定的人葡萄膜黑色素瘤小鼠肝内种植模型。种植的肿瘤细胞在裸鼠肝脏内呈浸润性生长,侵袭包括胃、十二指肠、脾脏及肾脏在内的多个器官,并可远端转移至肺脏。
[Abstract]:Aim: to study the biological behavior of human uveal malignant melanoma cell line (MUM2B) in vivo by intrahepatic injection of human uveal malignant melanoma strain (MUM2B cell). Methods: 5 脳 104 MUM2B cells were injected into the liver capsule of BALB/C-nu nude mice. For 6 weeks, 3 nude mice were randomly killed to observe the tumorigenesis rate and invasion rate. The tumor bearing liver, heart, spleen, lung, kidney, stomach and intestine of nude mice were collected. The tumor growth behavior in liver and its invasion and metastasis to other organs were examined by HE staining. Results: the tumorigenesis rate of the model was 83.3%. In the first 4 weeks after inoculation, the tumor grew infiltrating in the liver and did not invade the surrounding organs. At the 5th week after inoculation, the tumor began to invade the capsule of kidney, spleen, stomach and duodenum, and it could break through the capsule of stomach and intestine and invade and grow in its serous layer. At 6 weeks after inoculation, the tumor increased invasion of kidney, spleen, duodenum and stomach. Tumor cells could infiltrate the myometrium of duodenum of nude mice, invade the gastric myometrium and submucosa of nude mice, and even invade into the intramuscular layer of gastric mucosa. At the 6th week after inoculation, the tumor began to metastasize to the lung of nude mice, and metastasis could be found in hilar and parenchyma of all the tested nude mice, and the metastatic rate was 100%. No metastatic tumor foci were found in the other organs except the lung suggesting that the lung was the main metastatic target organ of the implanted tumor in the liver. In addition, after 2 weeks of bearing tumor, the proliferation of red myeloid in spleen of nude mice was obvious. A large number of megakaryocytes and myeloid granulocytes could be seen in the marrow of nude mice, showing leukaemia like reaction. After 4 weeks of bearing tumor, the star-like phenomenon in spleen was obvious and macrophage was active, suggesting that the histological changes of spleen in nude mice were closely related to the growth state of bearing tumor. Conclusion: intrahepatic injection of MUM2B cells in nude mice can establish a stable intrahepatic implantation model of human uveal melanoma mice. The implanted tumor cells infiltrate the liver of nude mice, invading many organs, including stomach, duodenum, spleen and kidney, and metastasis to the lung.
【作者单位】：南京工业大学生物与制药工程学院;南京中医药大学药学院;东南大学医学院病理学与病理生理学系;
【分类号】：R739.7

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