MMP-2在鼻息肉发病与复发机制中的作用
发布时间：2018-11-04 16:26
【摘要】：目的:研究基质金属蛋白酶-2(MMP-2)在鼻息肉与鼻息肉内镜手术后囊泡组织中的表达。 材料与方法:取10例鼻息肉、10例鼻息肉内镜术后囊泡组织以及10例单纯鼻中隔偏曲患者部分下鼻甲(对照组)。采用免疫荧光法观察MMP-2蛋白的定位及分布,采用实时定量PCR法检测MMP-2在三组组织中mRNA的表达,采用免疫印迹蛋白技术检测MMP-2在三组组织中蛋白的定量表达。 结果:1.息肉组与囊泡组上皮细胞、炎性细胞与腺上皮细胞MMP-2蛋白高表达,对照组部分腺上皮细胞MMP-2蛋白高表达;2.MMP-2在息肉组和囊泡组中的mRNA水平均比对照组增高(p0.05),MMP-2在息肉组与囊泡组中的mRNA水平无明显差异; 3.MMP-2在鼻息肉组和囊泡组中的蛋白水平均比对照组增高(p0.05),MMP-2在鼻息肉组和囊泡组中的蛋白水平无明显差异。 结论:MMP-2在鼻息肉与囊泡组织中的表达上调;术后抑制囊泡组织过度增生对预防鼻息肉复发有一定意义;研究MMP-2抑制物对鼻息肉的治疗有一定意义。
[Abstract]:Aim: to investigate the expression of matrix metalloproteinase-2 (MMP-2) in the vesicles of nasal polyps and nasal polyps after endoscopic surgery. Materials and methods: 10 cases of nasal polyps, 10 cases of nasal polyps after endoscopic surgery, and 10 cases of simple nasal septum deviation of partial inferior turbinate (control group). The localization and distribution of MMP-2 protein were observed by immunofluorescence, the expression of mRNA in three groups was detected by real-time quantitative PCR, and the protein expression of MMP-2 in three groups was detected by Western blot technique. The result is 1: 1. The expression of MMP-2 protein in the epithelial cells of polyp group and vesicle group, inflammatory cells and glandular epithelial cells was high, while that of MMP-2 protein in some glandular epithelial cells of the control group was higher than that in the control group. The mRNA level of 2.MMP-2 in polyp group and vesicle group was higher than that in control group (p0. 05). There was no significant difference in mRNA level between polyp group and vesicle group. The protein level of 3.MMP-2 in nasal polyp group and vesicle group was higher than that in control group (p0.05), but there was no significant difference in MMP-2 protein level in nasal polyp group and vesicle group. Conclusion: the expression of MMP-2 is up-regulated in nasal polyps and vesicles, the inhibition of excessive proliferation of vesicular tissue is of significance in preventing the recurrence of nasal polyps, and the study of MMP-2 inhibitors in the treatment of nasal polyps is of some significance.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R765.25
本文编号：2310438
[Abstract]:Aim: to investigate the expression of matrix metalloproteinase-2 (MMP-2) in the vesicles of nasal polyps and nasal polyps after endoscopic surgery. Materials and methods: 10 cases of nasal polyps, 10 cases of nasal polyps after endoscopic surgery, and 10 cases of simple nasal septum deviation of partial inferior turbinate (control group). The localization and distribution of MMP-2 protein were observed by immunofluorescence, the expression of mRNA in three groups was detected by real-time quantitative PCR, and the protein expression of MMP-2 in three groups was detected by Western blot technique. The result is 1: 1. The expression of MMP-2 protein in the epithelial cells of polyp group and vesicle group, inflammatory cells and glandular epithelial cells was high, while that of MMP-2 protein in some glandular epithelial cells of the control group was higher than that in the control group. The mRNA level of 2.MMP-2 in polyp group and vesicle group was higher than that in control group (p0. 05). There was no significant difference in mRNA level between polyp group and vesicle group. The protein level of 3.MMP-2 in nasal polyp group and vesicle group was higher than that in control group (p0.05), but there was no significant difference in MMP-2 protein level in nasal polyp group and vesicle group. Conclusion: the expression of MMP-2 is up-regulated in nasal polyps and vesicles, the inhibition of excessive proliferation of vesicular tissue is of significance in preventing the recurrence of nasal polyps, and the study of MMP-2 inhibitors in the treatment of nasal polyps is of some significance.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R765.25
【参考文献】
相关期刊论文 前4条
1 陈福权,黄维国,乔莉,姜鸿彦,邱建华;鼻息肉术后增生组织中转化生长因子α、β_1的表达和激素的作用[J];临床耳鼻咽喉科杂志;2002年08期
2 陆翔,刘争,崔永华;转化生长因子β_1和基质金属蛋白酶及其组织抑制物在慢性鼻窦炎和鼻息肉组织中的差异性表达[J];临床耳鼻咽喉科杂志;2005年14期
3 许庚,李源,谢民强,史剑波,李华斌;功能性内窥镜鼻窦手术后术腔粘膜转归阶段的划分及处理原则[J];中华耳鼻咽喉科杂志;1999年05期
4 陈文文,孙琴,邓亚新;复发性鼻息肉形成的临床及病理学观察[J];中华耳鼻咽喉科杂志;1997年01期
，本文编号：2310438
本文链接：https://www.wllwen.com/yixuelunwen/yank/2310438.html
最近更新
教材专著
