AG490防治后发性白内障的实验研究

发布时间：2018-11-04 21:48

【摘要】：目的通过在活体兔眼行白内障超声乳化术制造兔眼后发性白内障模型,并与正常兔和应用AG490的兔进行对照,研究AG490对后发性白内障的作用,并观察术后不同时间AG490对兔眼的影响及此药的毒副作用。方法将35只(70眼)成年健康新西兰大白兔随机分为3组:对照组:于前房内注入BrdU(0.02 mL);模型组:行透明晶状体超声乳化术,术中前房内注入BrdU(0.02 mL)+透明质酸钠(0.05 mL)+生理盐水(0.10 mL);治疗组:行透明晶状体超声乳化术,术中前房内注入BrdU(0.02 mL)+透明质酸钠(0.05 mL)+AG490(0.10 mL),术后1 d、3 d、7 d、1个月、2个月对3组兔眼进行裂隙灯显微镜、视网膜电图(electroretinogram,ERG)及病理组织学检查,免疫组织化学方法检测晶状体后囊膜上BrdU的表达。结果术后各时间点模型组与治疗组前房炎症反应情况及ERG检查差异均无统计学意义(均为P0.05)。治疗组后囊膜混浊发生率低于模型组,差异有统计学意义(P0.01)。各时间点治疗组赤道部及后囊膜晶状体上皮细胞均较模型组少。各时间点3组BrdU的表达差异均有统计学意义(均为P0.01)。术后1 d、3 d、7 d模型组与对照组及治疗组赤道部及前囊下阳性细胞核标记指数值比较差异均有统计学意义(均为P0.05),对照组与治疗组比较差异无统计学意义(均为P0.05);术后1个月、2个月各组间赤道部及前囊下阳性细胞核标记指数值两两比较差异均有统计学意义(均为P0.05)。结论 AG490可以通过抑制晶状体上皮细胞增殖而有效地延缓后发性白内障的发生。在2个月内的不同时间点AG490对兔眼均无明显的毒副作用。
[Abstract]:Objective to study the effect of AG490 on posterior cataract in rabbits by phacoemulsification in vivo and compared with normal rabbits and rabbits with AG490. The effects of AG490 on rabbit eyes at different time after operation and the toxicity and side effects of the drug were observed. Methods Thirty-five healthy adult New Zealand rabbits (70 eyes) were randomly divided into 3 groups: control group: BrdU (0.02 mL);) was injected into anterior chamber. Model group: hyaline lens phacoemulsification, intraoperative injection of BrdU (0.02 mL) sodium hyaluronate (0. 05 mL) saline (0. 10 mL);) The treatment group was treated with hyaline lens phacoemulsification. BrdU (0.02 mL) sodium hyaluronate (0. 05 mL) AG490 (0. 10 mL),) was injected into anterior chamber for 7 days, 1 month, 1 day after operation. Slit-lamp microscope, electroretinogram (electroretinogram,ERG) and histopathology were used to detect the expression of BrdU in the posterior capsule of the three groups. Results there was no significant difference in inflammatory response and ERG between the model group and the treatment group at each time point after operation (P0.05). The incidence of posterior capsule opacification in treatment group was lower than that in model group (P 0.01). The number of equatorial and posterior capsule lens epithelial cells in the treatment group was lower than that in the model group at each time point. There were significant differences in the expression of BrdU among the three groups at each time point (all P 0.01). There were significant differences in the positive nuclear marker index values between the model group and the control group and the treatment group on the 1st and 3rd day after operation (P0.05), and the difference was significant (P0.05) between the model group and the control group and the treatment group. There was no significant difference between the control group and the treatment group (P0.05). After 1 month and 2 months after operation, there were significant differences in the positive nuclear marker index values between the equator part and the anterior sac (P0.05). Conclusion AG490 can effectively delay the occurrence of posterior cataract by inhibiting the proliferation of lens epithelial cells. There were no significant side effects of AG490 on rabbit eyes at different time points within 2 months.
【作者单位】：秦皇岛市第一医院眼科;河北医科大学第二附属医院眼科;天津市南开医院眼科;北京市海淀区妇幼医院眼科;
【分类号】：R776.1

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