HIF-1和SDF-1在大鼠缺血再灌注视网膜中的表达及其意义

发布时间：2018-11-06 15:43

【摘要】：目的：探讨缺氧诱导因子-1a (hypoxia-inducible factor-1a, HIF-1a)和基质细胞衍生因子-1(stromal cell-derived factor-1, SDF-1)在缺血性视网膜疾病及视网膜缺血再灌注(retinal ischemia-reperfusion, RIR)发展中的表达规律及二者的相互关系。方法：选取56只眼前后节均正常的成年SD大鼠将其随机分为0,6,12,24,48,72,144h手术组和假手术组,其中手术组42只,每个时间点六只,假手术组14只,每个时间点2只,应用结扎大鼠颈总动脉的方法建立视网膜缺血再灌注动物模型.手术组结扎劲总动脉60分钟后恢复血流灌注,分别在再灌注0,6,12,24,48,72,144h后完整摘除眼球,剪除角膜,取出品状体,清除玻璃体,流水反复缓慢冲洗视网膜标本,酒精脱水石蜡包埋处理视网膜标本,应用免疫组织化学的方法和平均光密度法检测不同时相各组视网膜标本中HIF-1a和SDF-1阳性表达情况。结果：在视网膜缺血性再灌注假手术组中HIF-1a、SDF-1未见表达或轻微表达,手术组HIF-1a和SDF-1在0h开始表达,6h表达增强,12h表达最强,24h表达减弱,以后随着时间推移表达逐渐下降,手术组与假手术组相比差异具有明显的统计学意义(P0.01). HIF-1a和SDF-1表达的时相一致,具有正相关性。结论：缺氧诱导因子(HIF-1a)和基质细胞衍生因子(SDF-1)表达与视网膜缺血再灌注中视网膜功能损伤密切相关,同时二者具有协同相关性。
[Abstract]:Objective: to investigate the role of hypoxia inducible factor-1 (hypoxia-inducible factor-1a, HIF-1a) and stromal cell derived factor-1 (stromal cell-derived factor-1, SDF-1) in ischemic retinal diseases and retinal ischemia-reperfusion (retinal ischemia-reperfusion,). RIR) expression in the development of the law and the relationship between the two. Methods: Fifty-six adult SD rats with normal anterior and posterior segment were randomly divided into two groups: operation group (n = 42) and sham operation group (n = 2). There were 42 rats in operation group, 6 rats in each time point, 14 rats in sham operation group, 2 rats in each time point. The rat model of retinal ischemia reperfusion was established by ligating the common carotid artery. In the operation group, the blood flow was restored after 60 minutes of ligation of the common artery. After reperfusion, the eyeballs were removed completely, the cornea was cut off, the body was removed, the vitreous body was removed, and the retinal specimens were flushed by the water over and over again and slowly, respectively, after reperfusion, the eyeball was removed completely, the cornea was cut off, and the vitreous body was removed. The positive expression of HIF-1a and SDF-1 was detected by immunohistochemical method and mean optical density method. Results: there was no or slight expression of HIF-1a,SDF-1 in the retinal ischemic reperfusion sham-operation group. The expression of HIF-1a and SDF-1 began at 0 h, increased at 6 h, reached the peak at 12 h, and decreased at 24 h in the operation group. After that, the expression decreased gradually with the passage of time, the difference between the operation group and the sham operation group was statistically significant (P0.01). The expression of HIF-1a and SDF-1 were consistent and positively correlated. Conclusion: the expression of hypoxia-inducible factor (HIF-1a) and stromal cell-derived factor (SDF-1) are closely related to retinal function injury during retinal ischemia-reperfusion, and there is a synergistic correlation between them.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R774.1

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