p53、MDM2、eIF4E、EGFR在鼻咽癌组织中的表达及鼻咽癌临床生物学行为相关性的分析

发布时间：2018-11-15 16:27

【摘要】：目的：通过对p53、MDM2、eIF4E及EGFR在正常鼻咽部粘膜、鼻咽部非典型增生和鼻咽癌组织中表达的比较,分析这些基因异常与鼻咽癌患者的年龄、性别、不同病理类型、淋巴结转移等临床病理参数是否存在相关性；各异常基因之间是否具有相关性,从而探讨这些基因异常在鼻咽癌发生发展中的作用及其临床意义。方法：应用免疫组织化学两步法染色方法,对97例的鼻咽癌(其中未分化非角化型15例,分化非角化型82例),鼻咽部非典型增生18例及正常健康成人鼻咽粘膜17例病理标本检测p53、MDM2、eIF4E和EGFR的表达情况。所得数据采用PASW statistics 18.0的x2检验、Spearman相关分析进行统计处理。结果：p53、MDM2、eIF4E、EGFR在鼻咽癌、不典型增生、正常鼻咽粘膜中的表达有显著性差异(p＜0.05)。p53、MDM2、eIF4E、EGFR在不同年龄分组、不同性别及不同T分期亚组中的表达无显著性差异(p＞0.05)；MDM2与淋巴结转移具有明显相关性(p＜0.05)。eIF4E在不同分化程度的鼻咽癌组织中的表达具有显著差异性(p＜0.05)。p53与MDM2的表达具有相关性(p＜0.05)。结论： 1.鼻咽癌组织、非典型增生组织及正常粘膜膜组织中p53、MDM2、eIF4E、EGFR蛋白阳性表达率均依次降低,具有显著性差异,表明p53、MDM2、eIF4E、EGFR参与鼻咽癌的发生发展过程。 2.MDM2在鼻咽癌组织中的表达与淋巴结转移具有相关性。 3.eIF4E的表达与鼻咽癌的分化程度具有相关性。 4.p53与MDM2在鼻咽癌中的表达具有明显相关性,与EGFR、eIF4E无明显相关性。
[Abstract]:Objective: to compare the expression of p53 MDM2eIF4E and EGFR in normal nasopharyngeal mucosa, atypical hyperplasia of nasopharynx and nasopharyngeal carcinoma (NPC), and to analyze the relationship between these gene abnormalities and age, sex and different pathological types of patients with nasopharyngeal carcinoma (NPC). The correlation of clinicopathological parameters such as lymph node metastasis; To explore the role of these abnormal genes in the development of nasopharyngeal carcinoma and their clinical significance. Methods: 97 cases of nasopharyngeal carcinoma (including 15 cases of undifferentiated non-keratinized type and 82 cases of differentiated non-keratinized type) were studied by immunohistochemical two-step method. The expression of p53 MDM2eIF4E and EGFR in 18 cases of nasopharyngeal atypical hyperplasia and 17 cases of normal nasopharyngeal mucosa were detected. The data were analyzed by PASW statistics 18. 0 x 2 test and Spearman correlation analysis. Results: the expression of EGFR in nasopharyngeal carcinoma, atypical hyperplasia and normal nasopharyngeal mucosa was significantly different (p < 0.05). There was no significant difference in expression between different sex and T stage subgroups (p > 0.05). There was a significant correlation between MDM2 and lymph node metastasis (p < 0. 05). The expression of eIF4E was significantly different in nasopharyngeal carcinoma with different differentiation degree (p < 0. 05). The expression of p53 was correlated with MDM2 expression (p < 0. 05). Conclusion: 1. The positive expression rate of p53 MDM2EIF4EEGFR protein in nasopharyngeal carcinoma, atypical hyperplasia and normal mucosal membrane decreased in turn, with significant difference, indicating that p53 MDM2eIF4EEGFR is involved in the genesis and development of nasopharyngeal carcinoma. The expression of 2.MDM2 in nasopharyngeal carcinoma is correlated with lymph node metastasis. The expression of 3.eIF4E was correlated with the degree of differentiation of nasopharyngeal carcinoma. The expression of 4.p53 and MDM2 in nasopharyngeal carcinoma was significantly correlated with EGFR,eIF4E.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.63

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