超声造影剂微循环显影对家兔缺血性视神经病变血流动力学改变的研究

发布时间：2018-11-16 09:47

【摘要】：目的:探讨超声造影剂在家兔眼部睫状循环的显影并研究颈动脉狭窄与前部缺血性视神经病变(anterior ischemic optic neuropathy,AION)的关系。方法:将36只健康雄性家兔随机分为手术组(20只40眼)、假手术组(8只16眼)、空白对照组(8只16眼)。手术组采取家兔颈动脉部分缝合术建立颈动脉狭窄的动物模型,假手术组行颈动脉临时夹闭,空白组不做任何处理。分别于术后3、7、14、21 d和35 d行颈动脉彩色多普勒超声检查、超声造影剂睫状微循环显影与视神经组织病理学分析。结果:手术组家兔造模成功,术后均出现不同程度颈动脉狭窄。3、7、14、21 d和35 d颈动脉内径值分别为(0.74±0.15)、(0.74±0.15)、(0.81±0.20)、(0.88±0.20)mm和(1.05±0.29)mm,较假手术组与空白对照组低,差异无统计学意义(P0.05)。超声造影剂在睫状动脉显影良好,手术组单个超声微泡移动速度在各时间点分别为(7.10±2.12)、(8.20±2.39)、(8.11±2.24)、(7.46±2.19)mm/s和(10.11±3.14)mm/s,较假手术组及空白对照组低,差异有统计学意义(P0.05)。光学显微镜下可见手术组视神经纤维排列紊乱,轴突肿胀呈空泡状,细胞质着色浅,炎性细胞浸润。结论:通过超声造影剂微循环显影可观察到颈动脉狭窄的家兔睫状动脉血流速率下降,并出现视神经的组织病理学改变,提示颈动脉狭窄可以引起缺血性视神经病变。
[Abstract]:Objective: to study the relationship between carotid stenosis and anterior ischemic optic neuropathy (anterior ischemic optic neuropathy,AION). Methods: 36 healthy male rabbits were randomly divided into operation group (20 eyes, 40 eyes), sham operation group (8 eyes, 16 eyes) and blank control group (8 eyes, 16 eyes). The animal model of carotid artery stenosis was established in the operation group by partial suture of the carotid artery in rabbits, the temporary occlusion of the carotid artery was performed in the sham operation group, and no treatment was done in the blank group. Carotid color Doppler ultrasonography, ciliary microcirculation imaging and histopathological analysis of optic nerve were performed on 21 and 35 days after operation. Results: the model was successfully established in the operation group, and the carotid artery stenosis of different degree appeared after operation. The internal diameter of carotid artery was (0.74 卤0.15), (卤0.74 卤0.81 卤0.20) at 21 and 35 days after operation, respectively. (0. 88 卤0. 20) mm and (1. 05 卤0. 29) mm, were lower than those of sham operation group and blank control group (P0.05). Ultrasound contrast medium showed well in ciliary artery. The velocity of single ultrasound microbubble in operation group was (7.10 卤2.12), () 8.20 卤2.39), (8.11 卤2.24). (7.46 卤2.19) mm/s and (10.11 卤3.14) mm/s, were significantly lower than those in sham operation group and blank control group (P0.05). Under the optical microscope, the optic nerve fibers in the operation group were disordered, axon swelling was vacuolar, cytoplasm was light and inflammatory cells were infiltrated. Conclusion: the decrease of blood flow rate of ciliary artery and histopathological changes of optic nerve in rabbits with carotid artery stenosis can be observed by ultrasound contrast medium microcirculation, suggesting that carotid artery stenosis may cause ischemic optic neuropathy.
【作者单位】：重庆医科大学附属第二医院眼科;
【基金】：重庆市医学科研计划资助项目(编号:2010-1-26)
【分类号】：R774.6

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