α-硫辛酸对小鼠视网膜光化学损伤的保护作用
发布时间：2018-11-16 16:48
【摘要】：目的观察α-硫辛酸对光损伤后小鼠视网膜的保护作用。方法将42只成年健康BALB/C小鼠随机分为正常组、光损伤组和药物干预组（干预组又分为造模前后均给药组及造模后给药组）。除正常组外其他组小鼠均暴露于装有白色光源的光损伤箱内，光暴露时间为12h，之后暗室饲养12h，共3个循环，光照强度21400±1600 lx，总暴露时间为36h。箱内温度控制在25-29℃。药物干预组腹腔注射α-硫辛酸注射液100mg/Kg，一次/日，直至处死。分别于7d及14 d处死动物摘取眼球，制备眼球壁石蜡切片，在光学显微镜下观察其形态学变化，，测量视网膜外核层厚度，用TUNEL法计数外核层细胞凋亡数及凋亡指数，用x±s表示，各组之间比较用两因素析因设计的方差分析。结果与光损伤组相比，药物干预组视网膜结构层次分明，细胞排列整齐。其中视网膜外核层厚度，正常组41.62±0.77um,光损伤组7天30.51±0.73um，14天20.04±0.52um，药物干预组（C1组）7天40.52±0.80um，14天41.43±0.72um，造模后给药组（C2组）7天31.70±0.0.70um，14天38.92±1.43um，显著高于光损伤组，（p0.001）。Tunel试剂盒检测细胞凋亡结果显示：正常组AI1,光损伤组AI=15.44±1.72，药物干预Ⅰ组AI=7.23±0.65，药物干预Ⅱ组AI=7.48±0.88。给药组小鼠视网膜外核层的凋亡细胞数明显少于光损伤组小鼠。外核层（ONL）厚度及凋亡指数（AI）的组间差异具有统计学意义。结论在本实验条件下，α-硫辛酸对小鼠视网膜光损伤具有保护作用。
[Abstract]:Objective to observe the protective effect of 伪-lipoic acid on the retina of mice after light injury. Methods 42 healthy adult BALB/C mice were randomly divided into three groups: normal group, light injury group and drug intervention group. All the mice in the other groups except the normal group were exposed to light damage box with white light source for 12 hours, then the dark room was fed for 12 hours, and the light intensity was 21400 卤1600 lx,. The total exposure time was 36 hours. The temperature in the box is controlled at 25-29 鈩
本文编号：2336066
[Abstract]:Objective to observe the protective effect of 伪-lipoic acid on the retina of mice after light injury. Methods 42 healthy adult BALB/C mice were randomly divided into three groups: normal group, light injury group and drug intervention group. All the mice in the other groups except the normal group were exposed to light damage box with white light source for 12 hours, then the dark room was fed for 12 hours, and the light intensity was 21400 卤1600 lx,. The total exposure time was 36 hours. The temperature in the box is controlled at 25-29 鈩
本文编号：2336066
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