TLR4和IL-12在肥大腺样体中的表达及其在分泌性中耳炎中的意义

发布时间：2018-11-19 19:37

【摘要】： 目的：检测TLR4在儿童肥大的腺样体组织中的表达情况及IL-12在腺样体组织和鼓室积液中的含量,探讨腺样体免疫功能异常在分泌性中耳炎发病机制中的作用。方法：收集单纯腺样体肥大及伴有分泌性中耳炎的肥大腺样体标本各15例,同时收集单纯分泌性中耳炎及伴有腺样体肥大的鼓室积液标本各15例。应用逆转录聚合酶链反应(RT-PCR)技术检测TLR4在肥大腺样体中的表达情况,应用ELISA法测定鼓室积液及肥大腺样体组织中IL-12的含量。对各组间TLR4表达和IL-12含量的差异进行对比研究。结果：所有肥大腺样体组织中均有TLR4表达,个体之间差异较大。伴有分泌性中耳炎的肥大腺样体组织中TLR4 mRNA表达水平高于单纯腺样体肥大组(P0.05)；重度腺样体肥大者TLR4 mRNA表达水平显著高于与中度腺样体肥大组(P0.05)。伴有腺样体肥大的分泌性中耳炎组鼓室积液中IL-12含量低于单纯分泌性中耳炎组(P0.05),其腺样体组织中IL-12含量显著则高于单纯腺样体肥大组(P0.05)。腺样体肥大合并分泌性中耳炎者其腺样体组织中TLR4表达水平与IL-12含量呈正相关(r=0.901,P0.05)。结论：合并分泌性中耳炎和增殖程度较重的腺样体组织中TLR4表达增强；伴有腺样体肥大的分泌性中耳炎患儿鼓室积液中IL-12含量较单纯分泌性中耳炎者降低,合并分泌性中耳炎者腺样体组织匀浆中IL-12的含量较单纯腺样体肥大者增高；在合并分泌性中耳炎的腺样体肥大患者的腺样体组织中,TLR4的表达水平与IL-12的含量呈显著相关性。结果提示,TLR4介导的免疫反应可能通过调节鼻咽部和鼓室局部IL-12的含量在腺样体肥大导致分泌性中耳炎的发病机制中起作用。
[Abstract]:Aim: to investigate the expression of TLR4 in children's hypertrophic adenoid tissues and the content of IL-12 in adenoid tissue and tympanic effusion, and to explore the role of adenoid immune dysfunction in the pathogenesis of secretory otitis media. Methods: 15 cases of adenoid hypertrophy and 15 cases of hypertrophic adenoid with secretory otitis media, 15 cases of simple secretory otitis media and 15 cases of tympanic effusion with adenoid hypertrophy were collected. The expression of TLR4 in hypertrophic adenoids was detected by reverse transcriptase polymerase chain reaction (RT-PCR) and the content of IL-12 in tympanic effusion and hypertrophic adenoid tissue was determined by ELISA method. The expression of TLR4 and the content of IL-12 in each group were compared. Results: the expression of TLR4 was found in all hypertrophic adenoid tissues. The expression of TLR4 mRNA in hypertrophic adenoid tissue with secretory otitis media was higher than that in simple adenoid hypertrophy group (P0.05), and the TLR4 mRNA expression level in severe adenoid hypertrophy group was significantly higher than that in moderate adenoid hypertrophy group (P0.05). The content of IL-12 in tympanic effusion of secretory otitis media with adenoid hypertrophy was lower than that in simple secretory otitis media (P0.05), and the content of IL-12 in adenoid tissue was significantly higher than that in simple adenoid hypertrophy (P0.05). The expression of TLR4 in adenoid tissue was positively correlated with the content of IL-12 in adenoid hypertrophy complicated with secretory otitis media (P 0.05). Conclusion: the expression of TLR4 was increased in adenoids with secretory otitis media and proliferation. The content of IL-12 in tympanic effusion of children with secretory otitis media with adenoid hypertrophy was lower than that of patients with simple secretory otitis media. The content of IL-12 in adenoid tissue homogenate of patients with secretory otitis media was higher than that of patients with simple adenoid hypertrophy. There was a significant correlation between the expression of TLR4 and the content of IL-12 in adenoid hypertrophy patients with secretory otitis media. The results suggest that TLR4 mediated immune response may play a role in the pathogenesis of secretory otitis media caused by adenoid hypertrophy by regulating the content of IL-12 in nasopharynx and tympanum.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R764

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