依达拉奉对噪声性聋防治作用

发布时间：2018-11-22 13:05

【摘要】：目的探讨依达拉奉(Edaravone,Ed)对豚鼠声损伤的保护作用。方法清洁级豚鼠30只,雌雄不限,体重200~250g。随机分3组,正常对照组10只,生理盐水组10只,依达拉奉组10只。予白噪声120dB SPL,每天4h,连续4天。噪声前半小时腹腔注射依达拉奉溶液,8.0mg/kg,每天一次,持续给药到噪声完毕后一周。噪声前,噪声后即刻,第3天,第7天行听性脑干反应(ABR)检测,第8天琥珀酸脱氢酶,免疫组化,扫描电镜检测。结果噪声前,3组豚鼠ABR阈值无明显差异(P0.05),噪声后即刻生理盐水及依达拉奉组ABR阈值均高于正常对照组,依达拉奉组ABR阈值低于生理盐水组,差异有统计学意义(P0.05),3天后,依达拉奉组ABR阈值明显降低,7天后接近正常对照组,而生理盐水组则明显高于正常对照组,差异有统计学意义(P0.05)。正常对照组外毛细胞胞质琥珀酸脱氢酶(SDH)着色深,胞核、纤毛排列整齐;光镜下观察生理盐水组底转毛细胞中SDH着色浅,细胞肿胀不规则,依达拉奉组较生理盐水组外毛细胞胞质着色深,细胞形态规则,免疫组化及扫描电镜检测显示正常对照组细胞核纤毛排列整齐,未见缺失,而生理盐水组和依达拉奉组底转毛细胞出现缺失,依达拉奉组较生理盐水组细胞核及纤毛缺失明显减轻。结论结合之前的实验研究,初步推测依达拉奉对噪声性耳聋中产生的自由基具有清除作用。
[Abstract]:Objective to investigate the protective effect of Edaravone (Edaravone,Ed) on sound injury in guinea pigs. Methods Thirty clean grade guinea pigs, male and female, were used. They were randomly divided into 3 groups: normal control group (n = 10), normal saline group (n = 10) and Edaravone group (n = 10). White noise 120dB SPL, was given for 4 hours per day for 4 consecutive days. Edaravone solution, 8.0 mg / kg, was injected intraperitoneally half an hour before noise, once a day, until one week after the noise. Before, immediately after noise, on the 3rd and 7th day, the auditory brainstem response was detected by (ABR), and on the 8th day, succinate dehydrogenase was detected by immunohistochemistry and scanning electron microscopy. Results before noise, there was no significant difference in ABR threshold between the three groups (P0.05). The ABR threshold of normal saline group and Edaravone group was higher than that of normal control group immediately after noise (P0.05), and the ABR threshold of Edaravone group was lower than that of normal saline group. After 3 days, the ABR threshold of Edaravone group was significantly lower than that of normal control group after 7 days, while the normal saline group was significantly higher than that of normal control group (P0.05). In the control group, the cytoplasmic succinate dehydrogenase (SDH) staining was deep, the nuclei and ciliates were arranged neatly. Under the light microscope, the SDH staining in the basal hair cells of the saline group was light, and the cells were swollen irregularly. The cytoplasm of the hair cells in the Edaravone group was deeper than that in the saline group, and the morphology of the cells was regular. Immunohistochemistry and scanning electron microscopy showed that the nuclear cilia were arranged neatly in normal control group, but the loss of basal hair cells was found in normal saline group and Edaravone group. The deletion of nucleus and cilia in Edaravone group was significantly less than that in normal saline group. Conclusion Edaravone has scavenging effect on free radicals in noise induced deafness.
【作者单位】：温州医科大学第一临床医学院;温州解放军第118医院耳鼻咽喉科;解放军总医院耳鼻咽喉-头颈外科解放军耳鼻咽喉研究所;
【基金】：“全军十二五”面上项目(CWSlIJ256) 南京军区重大专项课题基金资助项目资助(10Z006,08Z007) 国家自然科学基金项目(81241034,81271081)支持
【分类号】：R764.43

【参考文献】