去氨加压素对耳蜗功能及上皮钠通道表达的调节作用

发布时间：2018-12-11 00:41

【摘要】：目的研究去氨加压素DDAVP调节豚鼠耳蜗功能及上皮钠通道蛋白α亚基(α-ENaC)的表达,探讨其在内淋巴积水中的作用。方法将24只豚鼠随机分为3组,分别给予0、5、10天DDAVP,通过耳蜗电图(ECochG)检测耳蜗功能学改变。应用苏木素-伊红染色(HE)确定各组耳蜗组织形态学改变,并用免疫组化法确定α-ENaC蛋白在耳蜗定位表达。采用蛋白印迹实验(WesternBlot)分别检测不同给药时间豚鼠耳蜗α-ENaC蛋白表达水平。结果DDAVP注射后,ECochG提示部分豚鼠耳蜗出现内淋巴积水,HE证实豚鼠内淋巴积水。α-ENaC蛋白在耳蜗血管纹、螺旋韧带、Reissner膜、Corti器、螺旋缘、螺旋神经、耳蜗神经表达。与未给予DDAVP相比,10天组豚鼠耳蜗α-ENaC蛋白表达升高(P0.05);。结论 DDAVP调节豚鼠耳蜗α-ENaC蛋白表达升高,进而调节内淋巴代谢,诱导内淋巴积水,影响内耳功能。
[Abstract]:Objective to study the effect of desmotensin DDAVP on cochlear function and expression of 伪-ENaC in guinea pig cochlea and its role in endolymphatic hydrops. Methods Twenty-four guinea pigs were randomly divided into 3 groups. The cochlear functional changes were detected by electrocochleogram (ECochG) after 10 days of DDAVP,. The histomorphology of cochlea in each group was determined by hematoxylin eosin staining (HE) and the expression of 伪-ENaC protein in cochlea was determined by immunohistochemical method. The expression of 伪-ENaC protein in cochlea of guinea pigs was detected by Western blot (WesternBlot). Results after DDAVP injection, ECochG revealed endolymphatic hydrops in cochlea of some guinea pigs, and HE confirmed endolymphatic hydrops in guinea pigs. 伪-ENaC protein was expressed in stria vascularis, helical ligament, Reissner membrane, Corti organ, spiral edge, spiral nerve and cochlear nerve. The expression of 伪-ENaC protein in cochlea of guinea pigs increased after 10 days compared with those without DDAVP (P0.05);.). Conclusion DDAVP can regulate the expression of 伪-ENaC in guinea pig cochlea, and then regulate the endolymphatic metabolism, induce endolymphatic hydrops and affect the inner ear function.
【作者单位】：重庆医科大学附属第一医院耳鼻咽喉科;
【基金】：国家临床重点专科建设项目经费资助(卫办医政函[2012]649号)
【分类号】：R764.33

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