糖尿病视网膜病变与外周血内皮祖细胞关系的研究及糖尿病视网膜病变神经纤维层厚度的研究
发布时间：2018-12-14 04:54
【摘要】：第一部分糖尿病视网膜病变与外周血内皮祖细胞关系的研究 目的:探讨不同时期糖尿病视网膜病变患者外周血中内皮祖细胞(EPCs)的数量变化,分析EPCs在DR的发展过程中的作用。 方法:20例健康对照组(Control组),22例无视网膜病变糖尿病患者(DM组),10例非增殖性糖尿病视网膜病变患者(NPDR组),12例增殖性糖尿病视网膜病变患者(PDR组),均采空腹肘前静脉血10ml,分别使用外周全血和单核细胞密度梯度离心法(Ficoll离心)分离单核细胞后,使用CD34+和CD309+双荧光抗体标记,通过流式细胞仪检测EPCs的数量,比较各组间EPCs数量的差异,实验结果采用SPSS 17.0统计软件处理。 结果:外周血中EPCs占淋巴细胞群的比例PDR组较其他三组显著增高,P=0.000,DM组与NPDR组相比,P=0.882,差异没有统计学意义。正常对照组高于DM组与NPDR组,差异有统计学意义。 结论:糖尿病视网膜病变组(DM组)和NPDR组患者外周血中EPCs的数量明显少于正常对照组;PDR组患者外周血中EPCs数量较Control组显著增加,EPCs可能与PDR时期眼底新生血管的发生有关。 第二部分糖尿病视网膜病变神经纤维层厚度的研究 目的:研究糖尿病视网膜病变(Diabetic Retinopathy;DR)及全视网膜激光光凝(Panretinal Photocoagulation;PRP)对视乳头旁视网膜神经纤维层(Retinal Nerve Fiber Layer;RNFL)厚度及黄斑中心凹视网膜厚度的影响。 方法:正常对照组(Control)34例66只眼,2型糖尿病无糖尿病视网膜病变(Diabetes Mellitus;DM)患者组20例32只眼,非增殖性糖尿病视网膜病变(NPDR)患者组20例26只眼,增殖性糖尿病视网膜病变(PDR)患者组19例20只眼,DR行PRP治疗组19例20只眼被纳入研究,应用光学相干断层扫描仪(Optical Coherence Tomography;OCT)测量视乳头旁神经纤维层厚度及黄斑中心凹视网膜厚度,比较各组间的差异。 结果: (1)PRP治疗后视乳头旁RNFL厚度与PDR组对比明显变薄,P=0.001;(2)黄斑区视网膜厚度明显增厚主要见于增殖性糖尿病视网膜病变(p0.05),PRP治疗后没有明显改变。 结论:糖尿病视网膜病变行PRP治疗后RNFL有所变薄,黄斑区视网膜厚度无明显改变。
[Abstract]:Part one the relationship between diabetic retinopathy and peripheral blood endothelial progenitor cells objective: to investigate the changes of (EPCs) in peripheral blood of patients with diabetic retinopathy at different stages. The role of EPCs in the development of DR is analyzed. Methods: twenty healthy controls (Control group), 22 patients without retinopathy diabetes mellitus (DM group), 10 patients with non-proliferative diabetic retinopathy (NPDR group), 12 patients with proliferative diabetic retinopathy (PDR group), 10 patients with non-proliferative diabetic retinopathy (NPDR group), 12 patients with proliferative diabetic retinopathy (PDR group) were enrolled in this study. 10 ml of fasting anterior cubital venous blood was collected. Monocytes were separated by peripheral blood and monocyte density gradient centrifugation (Ficoll) respectively. CD34 and CD309 double fluorescent antibodies were used to detect the number of EPCs by flow cytometry. The results of the experiment were processed by SPSS 17.0 software. Results: the ratio of EPCs in peripheral blood to lymphocyte group in PDR group was significantly higher than that in the other three groups. There was no significant difference between PDR group and NPDR group. Normal control group was higher than DM group and NPDR group, the difference was statistically significant. Conclusion: the number of EPCs in peripheral blood of diabetic retinopathy group (DM group) and NPDR group is significantly lower than that of normal control group, and that of PDR group is significantly higher than that of Control group. EPCs may be related to the occurrence of fundus neovascularization in PDR. The second part of the study on the thickness of nerve fiber layer in diabetic retinopathy objective: to study diabetic retinopathy (Diabetic Retinopathy;DR) and total retinal laser photocoagulation (Panretinal Photocoagulation;) The effect of PRP on the retinal nerve fiber layer (Retinal Nerve Fiber Layer;RNFL) thickness and macular fovea retinal thickness. Methods: in normal control group, there were 34 cases (66 eyes) of (Control), type 2 diabetes without diabetic retinopathy (Diabetes Mellitus;). DM (n = 20, n = 32), (NPDR) (n = 20, n = 26), (PDR) (n = 19, n = 20) and PRP (n = 19, n = 20). Applied optical coherence tomography (Optical Coherence Tomography; The thickness of parapapillary nerve fiber layer and macular fovea retina were measured by OCT. Results: (1) the thickness of parapapillary RNFL in PRP group was significantly thinner than that in PDR group. (2) Retinal thickening of macular area was mainly seen in proliferative diabetic retinopathy (p0. 05), PRP). Conclusion: RNFL became thinner and retinal thickness of macular area did not change after PRP treatment for diabetic retinopathy.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R774.1
本文编号：2377961
[Abstract]:Part one the relationship between diabetic retinopathy and peripheral blood endothelial progenitor cells objective: to investigate the changes of (EPCs) in peripheral blood of patients with diabetic retinopathy at different stages. The role of EPCs in the development of DR is analyzed. Methods: twenty healthy controls (Control group), 22 patients without retinopathy diabetes mellitus (DM group), 10 patients with non-proliferative diabetic retinopathy (NPDR group), 12 patients with proliferative diabetic retinopathy (PDR group), 10 patients with non-proliferative diabetic retinopathy (NPDR group), 12 patients with proliferative diabetic retinopathy (PDR group) were enrolled in this study. 10 ml of fasting anterior cubital venous blood was collected. Monocytes were separated by peripheral blood and monocyte density gradient centrifugation (Ficoll) respectively. CD34 and CD309 double fluorescent antibodies were used to detect the number of EPCs by flow cytometry. The results of the experiment were processed by SPSS 17.0 software. Results: the ratio of EPCs in peripheral blood to lymphocyte group in PDR group was significantly higher than that in the other three groups. There was no significant difference between PDR group and NPDR group. Normal control group was higher than DM group and NPDR group, the difference was statistically significant. Conclusion: the number of EPCs in peripheral blood of diabetic retinopathy group (DM group) and NPDR group is significantly lower than that of normal control group, and that of PDR group is significantly higher than that of Control group. EPCs may be related to the occurrence of fundus neovascularization in PDR. The second part of the study on the thickness of nerve fiber layer in diabetic retinopathy objective: to study diabetic retinopathy (Diabetic Retinopathy;DR) and total retinal laser photocoagulation (Panretinal Photocoagulation;) The effect of PRP on the retinal nerve fiber layer (Retinal Nerve Fiber Layer;RNFL) thickness and macular fovea retinal thickness. Methods: in normal control group, there were 34 cases (66 eyes) of (Control), type 2 diabetes without diabetic retinopathy (Diabetes Mellitus;). DM (n = 20, n = 32), (NPDR) (n = 20, n = 26), (PDR) (n = 19, n = 20) and PRP (n = 19, n = 20). Applied optical coherence tomography (Optical Coherence Tomography; The thickness of parapapillary nerve fiber layer and macular fovea retina were measured by OCT. Results: (1) the thickness of parapapillary RNFL in PRP group was significantly thinner than that in PDR group. (2) Retinal thickening of macular area was mainly seen in proliferative diabetic retinopathy (p0. 05), PRP). Conclusion: RNFL became thinner and retinal thickness of macular area did not change after PRP treatment for diabetic retinopathy.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R774.1
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