PTEN及P-ERK和P-AKT在中耳胆脂瘤上皮中的表达及意义

发布时间：2018-12-16 08:27

【摘要】：目的:检测蛋白酪氨酸磷酸酶基因(PTEN)、磷酸化蛋白激酶B(P-AKT)和磷酸化细胞外信号调节激酶(P-ERK)在人类中耳胆脂瘤上皮的表达情况及其相关性,探讨它们在中耳胆脂瘤形成机制中的重要作用。方法:应用免疫组织化学SABC法检测40例中耳胆脂瘤标本及15例正常皮肤标本中PTEN、P-AKT和P-ERK蛋白的表达量及定位。应用Western blot免疫印迹法检测其中20例中耳胆脂瘤标本和10例正常皮肤标本PTEN、P-AKT和P-ERK蛋白以及内参GAPDH的表达量。结果:①免疫组织化学显示,PTEN在胆脂瘤和正常皮肤中的细胞核及胞质均有着色。核PTEN在胆脂瘤的阳性表达率明显低于正常皮肤,两者差异具有统计学意义(P0.01);质PTEN在胆脂瘤的阳性表达率明显低于正常皮肤,两者差异具有统计学意义(P0.01);PAKT主要在细胞质着色,胆脂瘤中的阳性表达率明显高于正常皮肤,两者差异具有统计学意义(P0.01);PERK主要在细胞核着色,胆脂瘤中的阳性表达率明显高于正常皮肤,两者差异亦具有统计学意义(P0.01)。在中耳胆脂瘤标本中,PTEN分别与P-AKT、P-ERK蛋白的表达之间呈显著负相关(P0.01)。②Western blot免疫印迹法检测显示:胆脂瘤中PTEN的表达量明显少于正常皮肤中的表达量;而P-AKT和P-ERK在胆脂瘤中的表达量明显多于它们在正常皮肤中的表达量。结论:PTEN、P-AKT和P-ERK蛋白在中耳胆脂瘤中的异常表达可能与胆脂瘤上皮的高度增殖和抗凋亡密切相关。PTEN表达缺失导致其抑制作用减弱,一方面使P-AKT表达过度,继而引起胆脂瘤上皮细胞凋亡受抑制;同时也使P-ERK表达过度,导致胆脂瘤上皮细胞增殖加强。
[Abstract]:Objective: to investigate the expression of protein tyrosine phosphatase gene (PTEN), phosphorylated protein kinase B (P-AKT) and phosphorylated extracellular signal-regulated kinase (P-ERK) in human middle ear cholesteatoma epithelium. To explore their important role in the formation mechanism of middle ear cholesteatoma. Methods: the expression and localization of PTEN,P-AKT and P-ERK protein in 40 cases of middle ear cholesteatoma and 15 cases of normal skin were detected by immunohistochemical SABC method. The expression of PTEN,P-AKT, P-ERK and GAPDH in 20 cases of middle ear cholesteatoma and 10 cases of normal skin were detected by Western blot. Results: 1Immunohistochemistry showed that PTEN was stained in nuclei and cytoplasm of cholesteatoma and normal skin. The positive expression rate of nuclear PTEN in cholesteatoma was significantly lower than that in normal skin (P0.01), and the positive rate of PTEN in cholesteatoma was significantly lower than that in normal skin (P0.01). The positive expression rate of PAKT in cholesteatoma was significantly higher than that in normal skin (P0.01). The positive expression rate of PERK in cholesteatoma was significantly higher than that in normal skin (P0.01). In middle ear cholesteatoma, there was a significant negative correlation between the expression of PTEN and P-AKTtr P-ERK protein (P0.01). The expression of PTEN in cholesteatoma was significantly lower than that in normal skin (P0.01). The expression of P-AKT and P-ERK in cholesteatoma was higher than that in normal skin. Conclusion: the abnormal expression of PTEN,P-AKT and P-ERK proteins in middle ear cholesteatoma may be closely related to the high proliferation and anti-apoptosis of cholesteatoma epithelium. The lack of PTEN expression may weaken its inhibitory effect, on the one hand, make P-AKT overexpression. Then the apoptosis of cholesteatoma epithelial cells was inhibited. At the same time, it also overexpressed P-ERK, leading to the proliferation of cholesteatoma epithelial cells.
【作者单位】：中国医科大学附属盛京医院耳科;
【分类号】：R764.2

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