顺铂对鼻咽癌细胞rad51基因表达的影响

发布时间：2018-12-26 08:42

【摘要】：目的：通过检测RAD51基因表达情况，比较人鼻咽癌高分化鳞癌细胞（CNE1）、低分化鳞癌细胞（CNE2）与顺铂治疗的敏感性，探讨DNA修复基因RAD51是否能成为顺铂治疗鼻咽癌的有效靶点，并在其发生发展中起到的作用。方法：（1）采用Real-time quantitative PCR (RT-PCR)技术检测两组不同分化程度的鼻咽癌细胞与正常鼻粘膜上皮细胞中RAD51基因的表达情况，以及使用顺铂处理后的表达情况；（2）采用MTT方法检测并比较顺铂对不同分化程度鼻咽癌细胞的细胞存活率的影响。结果：（1）鼻咽癌高分化鳞癌细胞（CNE1）和鼻咽癌低分化鳞癌细胞（CNE2）中RAD51的mRNA水平均明显高于正常鼻粘膜组织细胞（P0.01），差异有统计学意义。（2）三个剂量组(4μmol/L组、8μmol/L组、16μmol/L组)顺铂干预鼻咽癌细胞后， CNE2细胞中RAD51的表达高于CNE1细胞，以4μmol/L组和8μmol/L组为甚（P0.05），差异有统计学意义。（3）同时，，各剂量组CNE1的细胞存活率，均低于CNE2（P0.01），差异有统计学意义。结论：RAD51在分化程度不同的鼻咽癌细胞中表达不同，在化疗药物刺激下，分化程度越低的鼻咽癌细胞，RAD51的表达越高，对顺铂化疗的敏感性越低。
[Abstract]:Objective: to compare the sensitivity of highly differentiated squamous cell carcinoma (CNE1), poorly differentiated squamous cell carcinoma (CNE2) and cisplatin in human nasopharyngeal carcinoma (NPC) by detecting the expression of RAD51 gene. To investigate whether DNA repair gene RAD51 can be an effective target for cisplatin in the treatment of nasopharyngeal carcinoma and its role in the pathogenesis and development of nasopharyngeal carcinoma. Methods: (1) Real-time quantitative PCR (RT-PCR technique was used to detect the expression of RAD51 gene in nasopharyngeal carcinoma (NPC) cells and normal nasal epithelial cells with different differentiation degree, and the expression of RAD51 gene after cisplatin treatment. (2) the effect of cisplatin on the survival rate of nasopharyngeal carcinoma cells with different differentiation was detected and compared by MTT. Results: (1) the mRNA levels of RAD51 in highly differentiated squamous cell carcinoma (CNE1) and poorly differentiated squamous cell carcinoma (CNE2) of nasopharyngeal carcinoma were significantly higher than those in normal nasal mucosa (P0.01). (2) after three dose groups (4 渭 mol/L group, 8 渭 mol/L group, 16 渭 mol/L group) treated with cisplatin, the expression of RAD51 in CNE2 cells was higher than that in CNE1 cells, especially in 4 渭 mol/L group and 8 渭 mol/L group (P0.05). (3) at the same time, the cell survival rate of CNE1 in each dose group was lower than that of CNE2 (P0.01), and the difference was statistically significant. Conclusion: the expression of RAD51 in nasopharyngeal carcinoma cells with different differentiation degree is different. Under the stimulation of chemotherapeutic drugs, the lower the differentiation degree, the higher the expression of RAD51 and the lower the sensitivity to cisplatin chemotherapy.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.63

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