高渗性海水和等渗性海水对变应性鼻炎小鼠鼻黏膜的影响

发布时间：2019-01-01 07:35

【摘要】：目的:研究高渗性海水(HS)和等渗性海水(IS)对变应性鼻炎(AR)小鼠鼻黏膜的影响,并初步探讨海水鼻腔盥洗治疗AR的机制。方法:将Der p1构建的BALB/c小鼠AR动物模型随机分为3组:HS组、IS组和空白对照组(BC组)。HS组和IS组造模完成后第1~14天分别给予希诺宁(NaCl 2.3%)和必通(NaCl0.9%)滴鼻,BC组造模完成后不予任何处理。阴性对照组不予任何处理,4组均在第14天末次给药后30min内计数小鼠喷嚏及挠鼻次数;并在末次给药24h后取各组小鼠鼻部组织固定,苏木精-伊红染色,通过透射电镜观察鼻黏膜微观结构。结果:AR小鼠经HS和IS滴鼻后,挠鼻及喷嚏次数较阴性对照组明显减少(P0.05);HS组、IS组小鼠鼻黏膜组织中嗜酸粒细胞与BC组比较差异无统计学意义(P0.05);HS组、IS组小鼠鼻黏膜纤毛柱状上皮细胞排列整齐,明显好于BC组,并且HS组小鼠鼻黏膜形态和微观结构较IS组更接近阴性对照组。结论:HS和IS鼻腔盥洗均可以促进损伤的鼻黏膜纤毛上皮修复,而且HS鼻腔盥洗促进损伤的鼻黏膜纤毛上皮修复作用好于IS。海水鼻腔盥洗治疗AR的作用机制可能是通过促进损伤的鼻黏膜纤毛上皮修复,从而减轻鼻部症状。
[Abstract]:Objective: to study the effects of hypertonic seawater (HS) and isosmotic seawater (IS) on nasal mucosa of (AR) mice with allergic rhinitis and to explore the mechanism of seawater nasal lavatory therapy for AR. Methods: the AR animal model of BALB/c mice constructed by Der p1 was randomly divided into three groups: HS group. IS group and blank control group (BC group,). HS group and IS group) were given sinonine (2.3%) and NaCl0.9% nasal drops 14 days after the completion of model making, and no treatment was given in BC group. No treatment was given in the negative control group. The number of sneezing and nasal scratching in the 30min was counted in the four groups after the last administration on the 14th day. The nasal tissue was fixed by hematoxylin-eosin staining and the microstructure of nasal mucosa was observed by transmission electron microscope 24 hours after the last administration. Results: the number of nasal scratches and sneezes in AR mice was significantly lower than that in negative control group after nasal drip with HS and IS (P0.05). There was no significant difference in eosinophils in nasal mucosa between IS group and BC group (P0.05). In HS group and IS group, the ciliated columnar epithelial cells of nasal mucosa were arranged neatly, which was better than that of BC group, and the morphology and microstructure of nasal mucosa in HS group were closer to negative control group than in IS group. Conclusion: both HS and IS can promote the repair of damaged nasal mucosal cilia epithelium, and HS nasal lavatory can promote the repair of injured nasal mucosal cilia epithelium better than IS.. The mechanism of seawater nasal lavage in the treatment of AR may be to alleviate nasal symptoms by promoting the repair of damaged ciliated epithelium of nasal mucosa.
【作者单位】：武汉大学人民医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金资助项目(No:81371070) 湖北省自然基金项目(No:2012FFB04434)
【分类号】：R765.21

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