鼻咽癌放疗后龋相关危险因素研究

发布时间：2019-01-05 12:34

【摘要】：目的 1.探讨鼻咽癌患者放疗后龋易感性及牙本质敏感程度的变化,分析其与放射龋之间的关系。 2.分析鼻咽癌患者放疗前后唾液蛋白总量及不同分子量蛋白含量变化,定量检测溶菌酶和乳铁蛋白含量,探讨各蛋白与放射龋之间的关系。方法 1.刃天青试纸法检测龋活性 2.VAS法评估牙本质敏感程度 3.BCA法测定唾液总蛋白含量；SDS-PAGE凝胶电泳分离唾液蛋白；Quantity One专业凝胶图像分析软件分析扫描图像,测得不同分子量蛋白条带的IOD值。 4.免疫印迹法检测唾液中溶菌酶和乳铁蛋白含量,化学发光法获得目标条带的X光胶片影像,Quantity One专业图像分析软件分析扫描图像,测得目标条带的IOD值。所有数据录入Excel建立数据库,根据原始资料多次进行核对以确保无误。实验结果导入SPSS16.0统计软件包进行统计学分析,采用重复测量资料方差分析和秩和检验,检验水准定为双侧α=0.05。结果 1.刃天青试纸法显示鼻咽癌患者放疗后龋活性增强(P0.05)。 2.鼻炎癌患者放疗后牙本质敏感度增加(P0.05)。 3.BCA法测定鼻咽癌病人非刺激性全唾液中总蛋白含量(μg/ml)结果显示：放疗后蛋白总量低于放疗前(P0.05,放疗前为875.04±114.93,放疗后为773.71±116.46)。SDS-PAGE凝胶电泳图片显示分子量约14.5 kD、28kD、38 kD、56 kD、80 kD位置的5种蛋白条带较稳定出现。其中分子量为14.5 kD、28kD、38 kD、56 kD的四种蛋白丰度值和百分含量放疗后均低于放疗前(P0.05)。80 kD蛋白丰度值和百分含量放疗后高于放疗前(P0.05)。 4.免疫印迹法检测鼻咽癌患者放疗前后唾液中溶菌酶和乳铁蛋白含量,结果显示：溶菌酶丰度值(IOD)和百分含量放疗后低于放疗前(P0.05,放疗前后丰度值分别为64.46±37.97、37.39±28.83;百分含量为6.80±2.20、4.58±1.59)；放疗前后乳铁蛋白丰度值和百分含量无明显统计学差异(P0.05,放疗前后丰度值分别是46.26±30.32、46.28±31.46；百分含量是2.50±1.62、2.82±1.62)。重复测量资料方差分析结果显示两种蛋白之间存在相互作用(P0.05)。结论 1.放疗后鼻咽癌患者的龋活性增强,同时牙本质的敏感程度增加。 2.鼻咽癌患者唾液中分子量为14.5kD、28 kD、38 kD、56 kD四种蛋白的丰度值和百分含量放疗后低于放疗前,80 kD的蛋白丰度值和百分含量放疗后高于放疗前。 3.溶菌酶丰度值和百分含量放疗后低于放疗前,乳铁蛋白丰度值和百分含量在放疗前后无明显改变。
[Abstract]:Objective 1. To investigate the changes of susceptibility to caries and dentin sensitivity in patients with nasopharyngeal carcinoma (NPC) after radiotherapy, and to analyze the relationship between them and radiation caries. 2. The changes of total salivary protein and different molecular weight proteins before and after radiotherapy in patients with nasopharyngeal carcinoma were analyzed. Lysozyme and lactoferrin contents were detected quantitatively and the relationship between each protein and radiation caries was discussed. Method 1. The sensitivity of dentin was evaluated by 2.VAS method, total protein content in saliva was determined by 3.BCA method, saliva protein was separated by SDS-PAGE gel electrophoresis. The scanning images were analyzed by Quantity One gel image analysis software, and the IOD values of different molecular weight protein bands were measured. 4. The contents of lysozyme and lactoferrin in saliva were detected by Western blotting. The scanning images were analyzed by, Quantity One software and the IOD values of target bands were measured by chemiluminescence. All data entered into the Excel to establish a database, according to the original data repeated checks to ensure that the correct. The experimental results were imported into SPSS16.0 statistical software package for statistical analysis, and repeated measurement data were used for variance analysis and rank sum test. The test level was set as bilateral 伪 = 0.05. Result 1. Edge azure test paper method showed that the caries activity of patients with nasopharyngeal carcinoma increased after radiotherapy (P0.05). 2. The sensitivity of dentin increased after radiotherapy in patients with rhinitis cancer (P0.05). The total protein content (渭 g/ml) in non-irritated whole saliva of nasopharyngeal carcinoma patients was determined by 3.BCA method. The results showed that the total protein content after radiotherapy was lower than that before radiotherapy (P 0.05, 875.04 卤114.93 before radiotherapy). After radiotherapy, 773.71 卤116.46). SDS-PAGE gel electrophoresis images showed that five protein bands with molecular weight of about 14. 5 kD,28kD,38 kD,56 kD,80 kD appeared stably. The protein abundance and percentage content of the four proteins with molecular weight of 14. 5 kD,28kD,38 kD,56 kD were lower after radiotherapy than before radiotherapy (P0.05). 80 kD protein abundance and percentage content after radiotherapy were higher than those before radiotherapy (P0.05). 4. The levels of lysozyme and lactoferrin in saliva of patients with nasopharyngeal carcinoma before and after radiotherapy were detected by Western blotting. The results showed that the abundance of lysozyme (IOD) and percentage after radiotherapy were lower than those before radiotherapy (P 0.05). The abundance before and after radiotherapy was 64.46 卤37.97 卤37.39 卤28.83; The percentage content was 6.80 卤2.20 卤4.58 卤1.59). There was no significant difference in the abundance and percentage of lactoferrin before and after radiotherapy (P 0.05, 46.26 卤30.32 卤46.28 卤31.46 and 2.50 卤1.622 卤2.82 卤1.62, respectively). The results of ANOVA showed that there was interaction between the two proteins (P0.05). Conclusion 1. After radiotherapy, the caries activity of nasopharyngeal carcinoma patients increased and the sensitivity of dentin increased. 2. The abundance and percentage of four proteins in saliva of nasopharyngeal carcinoma patients with molecular weight of 14.5 kDX 28 kD,38 kD,56 kD were lower after radiotherapy than those before radiotherapy, and the protein abundance and percentage content of 80 kD after radiotherapy were higher than those before radiotherapy. 3. The abundance and percentage of lysozyme after radiotherapy were lower than those before radiotherapy, but lactoferrin abundance and percentage did not change significantly before and after radiotherapy.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.63

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