成人重度OSAHS患者血清中HSP70和MDA的含量及意义

发布时间：2019-03-09 17:44

【摘要】：目的通过检测成人重度OSAHS患者血清中HSP70和MDA的含量,比较重度OSAHS患者干预(CPAP和改良UPPP术等治疗)前后分别与正常对照组之间HSP70和MDA含量有无差异;比较重度OSAHS患者干预前后之间的LSaO2、AHI、HSP70和MDA含量有无差异。分析HSP70和MDA的相关性;HSP70、MDA分别与AHI之间的相关性;HSP70、MDA分别与LSaO2之间的相关性。初步探讨OSAHS病情轻重程度对HSP70和MDA含量的影响;干预因素对HSP70和MDA含量的影响;HSP70在OSAHS中是否存在着广泛的抗氧化应激作用; HSP70和MDA是否可以作为指标,更好地指导OSAHS的诊疗和评价预后。方法对所有入选对象行PSG监测。正常对照组50例。实验组50例,诊断为重度OSAHS。实验组患者干预(CPAP和改良UPPP术等治疗)前和干预后复查时(6个月后复查)早上7:30空腹抽静脉血取血清,正常对照组早上7:30空腹抽静脉血取血清,ELISA法检测HSP70含量,硫代巴比妥酸(TBA)法检测MDA含量,分别进行正常对照组、干预前和干预后的比较,分析HSP70、MDA和AHI、LSaO2之间的相关性。运用SPSS18.0统计软件对所有相关数据进行处理分析。结果 1.正常对照组和重度OSAHS患者HSP70和MDA含量的比较,正常对照组的含量要低于重度干预前患者的含量,差异有统计学意义(P0.01);正常对照组的含量要低于重度干预后患者的含量,差异有统计学意义(P0.01)。 2.重度OSAHS患者干预前后HSP70和MDA含量的比较,重度患者干预前含量要高于干预后的含量,差异有统计学意义(P0.01)。 3.重度OSAHS患者干预前后AHI值比较,重度患者干预前要高于干预后,差异有统计学意义(P0.01)。 4.重度OSAHS患者干预前后最低血氧饱和度值比较,重度患者干预前要低于干预后,差异有统计学意义(P0.01)。 5.运用直线相关和直线回归分析,发现HSP70和MDA含量存在直线正相关,有统计学意义(P0.01);HSP70和MDA分别与AHI存在直线正相关,有统计学意义(P0.01);HSP70和MDA分别与LSaO2存在直线负相关,有统计学意义(P0.01)。结论 1.有效地治疗OSAHS,可以降低HSP70和MDA的水平,改善体内氧化应激状态和减轻低氧血症。 2. HSP70和MDA之间有直线关系,并呈正相关,都随着OSAHS患者病情加重而相应地升高。两者可作为评估OSAHS患者病情轻重和术后疗效的指标。 3.HSP70在OSAHS体内可能起着广泛的抗氧化应激的作用。 4.手术和CPAP治疗是改善OSAHS患者体内氧化应激状态有效的治疗手段。
[Abstract]:Objective to detect the levels of HSP70 and MDA in serum of adult patients with severe OSAHS and to compare the contents of HSP70 and MDA between the patients with severe OSAHS before and after intervention (CPAP and modified UPPP procedure) and those in the normal control group. The levels of LSaO2,AHI,HSP70 and MDA in patients with severe OSAHS were compared before and after intervention. The correlation between HSP70 and MDA, between HSP70,MDA and AHI, and between HSP70,MDA and LSaO2 were analyzed. To explore the influence of the severity of OSAHS on the content of HSP70 and MDA, the influence of intervention factors on the content of HSP70 and MDA, the existence of extensive anti-oxidative stress in OSAHS, and the influence of intervention factors on the content of HSP70 and MDA. Whether HSP70 and MDA can be used as indicators to better guide the diagnosis and treatment of OSAHS and evaluate the prognosis. Methods all subjects were monitored by PSG. Normal control group (n = 50). Experimental group (50 cases) diagnosed as severe OSAHS. Before and after the intervention (CPAP and modified UPPP procedure, etc.), the serum was drawn from the fasting blood at 7:30 and 7:30 in the normal control group, and the HSP70 content was measured by ELISA method, while the serum was drawn at 7:30 before and after the intervention (6 months after the intervention), and the serum was drawn from the fasting vein blood at 7:30 in the normal control group. The content of MDA was detected by thiobarbituric acid (TBA) method. The correlation between HSP70,MDA and AHI,LSaO2 was analyzed by comparison of HSP70,MDA and AHI,LSaO2 in normal control group before and after intervention. SPSS18.0 statistical software was used to process and analyze all relevant data. Outcome 1. The contents of HSP70 and MDA in normal control group and severe OSAHS patients were lower than those before severe intervention, the difference was statistically significant (P0.01). The content of normal control group was lower than that of patients after severe intervention, the difference was statistically significant (P0.01). 2. The contents of HSP70 and MDA in patients with severe OSAHS before and after intervention were higher than those after intervention, the difference was statistically significant (P0.01). 3. The AHI value of severe OSAHS patients before and after intervention was higher than that of post-intervention, and the difference was statistically significant (P0.01). 4. The lowest blood oxygen saturation before and after intervention in severe OSAHS patients was significantly lower than that in severe patients before and after intervention (P0.01). 5. Using linear correlation and linear regression analysis, it was found that there was a linear positive correlation between HSP70 and MDA (P0.01), while HSP70 and MDA were positively correlated with AHI (P0.01). There was a negative linear correlation between HSP70 and MDA and LSaO2 (P0.01). Conclusion 1. Effective treatment of OSAHS, can reduce the levels of HSP70 and MDA, improve the oxidative stress and reduce hypoxemia. 2. There was a linear relationship between HSP70 and MDA, and there was a positive correlation, both of which increased with the aggravation of OSAHS. Both of them can be used as indexes to evaluate the severity of OSAHS patients and postoperative curative effect. 3.HSP70 may play an important role in anti-oxidative stress in OSAHS. 4. Surgery and CPAP therapy are effective methods to improve oxidative stress in OSAHS patients.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R766

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