环磷酰胺对豚鼠自身免疫性感音神经性聋听功能的影响

发布时间：2019-03-26 13:57

【摘要】：目的探讨环磷酰胺在制备豚鼠自身免疫性耳聋动物模型中的作用。方法白色红目豚鼠30只,配对设计分成3组各10只。实验组豚鼠腹腔注射环磷酰胺(150mg/kg)进行预处理,2 d后,每只豚鼠将粗制内耳抗原(crude in-ner ear antigens,CIEAgs)400μg·(0.2ml)-1加等量完全弗氏佐剂进行后足垫、背部皮内多点注射免疫;对照1组用CIEAgs 400μg·(0.2ml)-1加等量完全弗氏佐剂免疫动物;对照2组仅行环磷酰胺预处理。所有动物免疫前、免疫后7、14、21d,接受听性脑干诱发电位(auditory brain-stem response,ABR)检测。结果免疫前所有豚鼠ABR反应阈均值为29.50±5.56dBSPL,免疫后7天实验组、对照1及对照2组ABR反应阈分别为52.50±12.72、36.50±11.25、31.50±3.37;免疫后14天时三组反应域分别为58.50±14.79、33.25±8.93、30.50±3.69 dBSPL;免疫后21天分别为46.25±12.45、30.00±4.29、30.00±3.33 dBSPL。免疫后,实验组动物各时间段反应阈高于对照1,2组(P0.01)。免疫前后组内比较,实验组免疫后7天、14天、21天反应域高于实验前(P0.01);对照1组仅免疫后7天高于免疫前(P0.05),14天、21天与免疫前相比差异无显著性,对照2组各时间段与免疫前相比差异均无显著性。结论将豚鼠用环磷酰胺进行预处理,可以显著提高粗制内耳抗原建立自身免疫性耳聋动物模型的成功率,并显著提高其ABR阈值,而环磷酰胺本身对豚鼠内耳听力没有显著影响。
[Abstract]:Objective To study the role of cyclophosphamide in the preparation of an animal model of autoimmune deafness in guinea pigs. Methods Thirty-three white-red guinea pigs were divided into three groups, each of which was divided into three groups. In the experimental group, the guinea pig was pretreated by intraperitoneal injection of cyclophosphamide (150 mg/ kg), and after 2 days, the crude inner ear antigen (CIEAgs) was 400. m u.g 路 (0.2 ml) -1 with the same amount of complete Freund's adjuvant, and the back-foot pad and the back were injected with multiple points. The control group was immunized with CIEAgs 400. m. g. (0.2 ml) -1 with the same amount of complete Freund's adjuvant. All animals were pre-immunized,7,14 and 21 days after the immunization, and the auditory brainstem response (ABR) was detected. Results The mean value of ABR in all guinea pigs was 29.50 and 5.56 dBSPL. The response threshold of ABR in control group and control group was 52.50, 12.72, 36.50, 11.25, 31.50 and 3.37, respectively. The response domains of the three groups were 58.50, 14.79, 33.25, 8.93, 30.50 and 3.69dBSPL, respectively. The 21 days after immunization were 46.25, 12.45, 30.00, 4.29, 30.00 and 3.33 dBSPL, respectively. After immunization, the reaction threshold of the experimental group was higher than that of control group 1 and 2 (P0.01). Compared with the pre-and post-immunization group, the reaction domain of the control group was higher than that before the experiment (P0.01). The difference between the first group and the control group was higher than that of the pre-immunization group (P0.05). The difference between the control group and the control group was no significant. Conclusion The treatment of the guinea pig with cyclophosphamide can significantly improve the success rate of the animal model of self-immune hearing loss in the crude inner ear antigen, and significantly increase the ABR threshold, and the cyclic fosfamine itself has no significant effect on the hearing of the inner ear of the guinea pig.
【作者单位】：安徽省立医院耳鼻咽喉头颈外科;
【基金】：安徽省自然科学基金面上项目(11040606M221)
【分类号】：R764.431

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