宽频带白噪声适度暴露对小鼠听力损害的实验研究

发布时间：2019-04-02 21:38

【摘要】：目的通过100dB SPL宽频带白噪声对成年C57小鼠进行单次2小时的暴露,观察该种噪声对小鼠听功能损害的特点。方法选取5~6周龄ABR听阈正常的C57小鼠(每组5-7只),随机分为5个实验组分别为:噪声暴露后即刻(P0)、1天(P1)、3天(P3)、7天(P7)、14天(P14)。实验组小鼠于100dB SPL宽频带白噪声环境下暴露2h,本实验采用噪声暴露前的小鼠作为对照组。每只实验小鼠分别于噪声暴露前及暴露后各时间点进行ABR阈值检测以评估听力受损情况,实验采用统计噪声暴露前、后各时间点小鼠ABR差值(阈移)的方法分析实验结果。结果噪声暴露后即刻,小鼠各频率的ABR阈移均为最大值(P0.01):Click(11.92±7.51dB)、4kHz(11.92±5.60dB)、8kHz(12.31±5.99dB)、16kHz(21.54±8.75dB)、32kHz(20.00±8.90dB),且在各个频率的阈移中,以16kHz及32kHz处的高频听力损失最严重,明显高于Click、4kHz及8kHz处的阈移(P0.05)。脱离噪声环境24小时后各频率的ABR阈移开始下降,2周以后,小鼠各频率ABR阈值均完全恢复至暴露前水平(P0.05):Click(1.11±6.01dB)、4kHz(1.11±3.33dB)、8kHz(1.11±4.17dB)、16kHz(1.67±4.33)、32kHz(2.78±4.41)。结论 100dB SPL宽频带白噪声单次有限暴露可以造成小鼠出现一定程度的听力损害。在频率分布上,高频区域的听力损害更为明显。同时,本研究表明,这种噪声下小鼠听力损害可以表现自我主动恢复的特征。
[Abstract]:Aim to observe the characteristics of hearing impairment in adult C57 mice exposed to 100dB SPL wide band white noise for 2 hours. Methods C57 mice aged 5-6 weeks with normal ABR hearing threshold were randomly divided into five experimental groups: immediately after noise exposure (P0), one day (P1), three days (P3), seven days (P7), and fourteen days (P14). The mice were randomly divided into five groups: immediately after noise exposure (P0), one day (P1), three days (P3), seven days (P7), and fourteen days (P14). The mice in the experimental group were exposed to 100dB SPL wide band white noise for 2 hours. The mice before noise exposure were used as the control group. ABR threshold values were measured before and after noise exposure in each mouse to evaluate hearing impairment. The results were analyzed by statistical method of ABR difference (threshold shift) at each time point before and after noise exposure. Results immediately after noise exposure, the threshold shift of ABR at each frequency was maximum (P0.01): Click (11.92 卤7.51 dB), 4kHz (11.92 卤5.60 dB), 8kHz (12.31 table 5.99 dB), 16kHz (21.54 卤8.75 dB), 32kHz (20.00 卤8.90 dB). In each frequency threshold shift, the high frequency hearing loss at 16kHz and 32kHz was the most serious, which was significantly higher than that at Click,4kHz and 8kHz (P0.05). The threshold shift of ABR at each frequency began to decrease 24 hours after noise removal. After 2 weeks, the threshold of ABR at each frequency recovered completely to the pre-exposure level (P0.05): Click (1.11 卤6.01 dB) and 4kHz (1.11 卤3.33 dB). 8kHz (1.11 卤4.17dB), 16kHz (1.67 卤4.33), 32kHz (2.78 卤4.41). Conclusion single limited exposure to 100dB SPL broadband white noise can induce certain degree of hearing impairment in mice. In the frequency distribution, the hearing damage in the high frequency region is more obvious. At the same time, this study shows that this noise can show the characteristics of self-active recovery in mice hearing impairment.
【作者单位】：中国医科大学附属第一医院耳鼻咽喉头颈外科;解放军总医院耳鼻咽喉头颈外科解放军耳鼻咽喉研究所;
【基金】：辽宁省科技计划项目(No.2011225017,2012225021) 国家重大基础研究项目(973项目:2012CB967900;2012CB967901) 中国医科大学附属第一医院科学研究基金(FSFH 1216) 北京自然科学基金(5122040) 中国博士后科学基金(201003779;20100470103) 国家自然科学基金(NSFC,31040038)
【分类号】：R764.43

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