神经生长因子对木糖诱导白内障大鼠晶状体的保护作用

发布时间：2019-04-03 15:38

【摘要】：目的通过研究正常状态下及木糖诱导的白内障大鼠晶状体中神经生长因子(NGF)的存在、表达,来探讨白内障的发病机制。方法将40只Wistar大鼠随机分为4组,分别取晶状体后用生理盐水冲洗、新洁尔灭消毒后放入试管中,空白对照组试管中加入无血清199培养基,模型组加入D-木糖,对照组加入生理盐水,NGF组加入外源性NGF。给予相应处理后,观察各组中晶状体的浑浊度,酶联免疫吸附法(ELISA)法检测24、48、72 h空白对照组与模型组中内源性NGF含量。结果裂隙灯下观察模型组24、48、72 h晶状体浑浊系数明显高于空白对照组(P0.05)。NGF组24、48、72 h晶状体浑浊系数明显低于对照组(P0.05)。空白对照组24、48、72 h NGF在晶状体中的含量微弱,模型组24、48、72 h NGF在晶状体中的含量明显高于空白对照组(P0.05)。随时间推移,NGF含量呈下降趋势,但其含量仍然明显高于空白对照组(P0.05)。结论 NGF具有减缓白内障发展的功能,白内障的发展变化过程与晶状体中NGF的减少有关。
[Abstract]:Aim to investigate the pathogenesis of cataract by studying the presence and expression of nerve growth factor (NGF) in lens of normal and xylose-induced cataract rats. Methods 40 Wistar rats were randomly divided into 4 groups: lens extraction, washing with normal saline, disinfecting with new germicidal, adding serum-free medium to blank control group, D-xylose in model group, serum-free medium in blank control group and D-xylose in model group. Normal saline was added to the control group and exogenous NGF. was added to the NGF group. After treatment, the turbidity of lens in each group was observed, and the endogenous NGF content in control group and model group was detected by enzyme-linked immunosorbent assay (ELISA) for 24, 48, 72 hours. Results the lens opacification coefficient of the model group observed under slit lamp was significantly higher than that of the blank control group at 24,48,72 h (P0.05). NGF group 24,48,72 h) was significantly lower than that of the control group (P0.05). The content of NGF in lens of blank control group was weak at 24,48,72 hours. The content of NGF in lens of model group at 24,48,72 hours was significantly higher than that of blank control group (P0.05). With the passage of time, the content of NGF decreased, but its content was still significantly higher than that of the blank control group (P0.05). Conclusion NGF has the function of slowing down the development of cataract. The development and change of cataract is related to the decrease of NGF in lens.
【作者单位】：佳木斯大学附属第一医院眼科;
【基金】：黑龙江省卫生厅科研课题(2013257)
【分类号】：R776.1

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