重组PGC-1α蛋白调控小鼠视网膜新生血管的形成

发布时间：2019-04-28 11:07

【摘要】：目的:探讨重组过氧化物酶增殖激活受体-γ共激活子-1α(peroxisome-proliferator-activated receptor-γcoactivator-1α,PGC-1α)蛋白对小鼠视网膜新生血管形成的调控作用。方法:选7 d龄C57BL/6J小鼠80只,其中40只为正常组,随机分为正常注药组和正常对照组;另40只建立缺氧诱导的视网膜新生血管模型,随机分为模型注药组和模型对照组。向出生后12 d(postnatal day 12,P12)的正常注药组和模型注药组小鼠玻璃体腔注射重组PGC-1α蛋白,对照组不作注射。采用荧光造影视网膜铺片方法观察血管形态变化;取组织切片观察并计算突破视网膜内界膜的血管内皮细胞核数;以实时定量PCR方法检测视网膜血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)的表达;Western印迹方法检测视网膜PGC-1α和VEGF的表达。结果:视网膜铺片结果显示正常对照组未见明显新生血管,而正常注药组可见新生血管,模型注药组较模型对照组新生血管丛增多,荧光渗漏加重;组织切片可见正常注药组和模型注药组分别较对照组突破视网膜内界膜的细胞核数量增多(P0.01);视网膜PGC-1α蛋白表达水平在正常注药组和模型注药组中均较对照组明显上调(P0.01);视网膜VEGF m RNA及蛋白表达水平在正常注药组和模型注药组中均较对照组明显上调(P0.01)。结论:PGC-1α能促进小鼠视网膜新生血管的形成。
[Abstract]:Aim: to investigate the regulatory effect of peroxisome-proliferator-activated receptor- 纬 coactivator-1 伪 (PGC-1 伪) protein on retinal neovascularization in mice. Methods: 80 7-day-old C57BL/6J mice were randomly divided into normal group (n = 40) and normal control group (n = 40), and the model of retinal neovascularization induced by hypoxia (n = 40) was randomly divided into two groups: model group (n = 40) and control group (n = 40). Recombinant PGC-1 伪 protein was injected into the vitreous cavity of the normal and model mice injected with 12 d (postnatal day 12, P 12 (P 12) after birth, while the control group was not injected with the recombinant PGC-1 伪 protein. The morphological changes of blood vessels were observed by fluorescein angiography, and the number of endothelial cell nuclei breaking through the inner limiting membrane of retina were observed and calculated by tissue sections. Real-time quantitative PCR was used to detect the expression of vascular endothelial growth factor (vascular endothelial growth factor,VEGF, and Western blot was used to detect the expression of PGC-1 伪 and VEGF in retina. Results: there was no obvious neovascularization in the normal control group, but the neovascularization could be seen in the normal drug injection group. The neovascularization in the model injection group was more than that in the model control group, and the fluorescence leakage was aggravated. Tissue sections showed that the number of nuclei breaking through the inner limiting membrane of retina in the normal drug injection group and the model drug injection group was higher than that in the control group (P0.01). The expression level of PGC-1 伪 protein in retina was up-regulated in normal group and model group as compared with that in control group (P0.01). The expression of VEGF m RNA and protein in retina were up-regulated in normal group and model group compared with control group (P0.01). Conclusion: PGC-1 伪 can promote the formation of retinal neovascularization in mice.
【作者单位】：中南大学湘雅医院眼科;湖南省儿童医院眼科;
【基金】：国家自然科学基金青年科学基金(81000387) 教育部博士点基金(20100162120050)~~
【分类号】：R774.1

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