不同程度间歇性低氧对大鼠不同脑区Bax、Bcl-2表达的影响

发布时间：2019-05-19 21:58

【摘要】：目的观察不同程度间歇性低氧后大鼠不同脑区Bax、Bcl-2表达变化,探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)神经损伤机制。方法 30只雄性SD大鼠随机分成对照组、轻度间歇低氧组、重度间歇低氧组各10只。对照组暴露于空气中,间歇低氧组分别暴露于不同低氧条件下(100 ml/L和50 ml/L,暴露时间每天8 h,持续时间2 w和8 w)。光镜观察额叶皮质、纹状体、海马区和小脑神经细胞结构变化,免疫组化检测上述区域Bax、Bcl-2表达变化。结果与对照组比较,随低氧时间的延长,两组间歇性低氧组大鼠额叶皮质、纹状体、海马区和小脑神经细胞区死亡神经元密度增高;Bcl-2/Bax比值降低(P0.05);上述变化在重度间歇性低氧组最为显著(P0.05);轻度间歇性低氧组各脑区死亡神经元密度、Bcl-2/Bax比值变化差异显著,以额叶皮质和海马最明显(P0.05);重度间歇性低氧组各脑区死亡神经元密度、Bcl-2/Bax比值变化差异显著,以皮质、海马和纹状体区最明显。结论间歇性低氧可引起不同脑区Bcl-2和Bax差异性表达、神经细胞差异性丢失;皮质、海马和纹状体对间歇性低氧损伤更敏感。
[Abstract]:Objective to observe the changes of Bax,Bcl-2 expression in different brain regions of rats after intermittent hypoxia and to explore the mechanism of (OSAHS) nerve injury in obstructive sleep apnea hypopnea syndrome. Methods 30 male SD rats were randomly divided into control group (n = 10), mild intermittent hypoxia group (n = 10) and severe intermittent hypoxia group (n = 10). The control group was exposed to air, and the intermittent hypoxia group was exposed to different hypoxia conditions (100 ml/L and 50 ml/L, exposure time 8 h, duration 2 w and 8 w)., respectively). The structural changes of nerve cells in frontalis cortex, striatum, hippocampus and cerebellum were observed by light microscope, and the expression of Bax,Bcl-2 in these areas was detected by immunohistochemistry. Results compared with the control group, with the prolongation of hypoxia time, the density of dead neurons in the frontolobe cortex, striatum, hippocampus and cerebellar nerve cell area of the two groups increased, and the ratio of Bcl-2/Bax decreased (P 0.05). The above changes were most significant in the severe intermittent hypoxia group (P 0.05), and the death neuron density and Bcl-2/Bax ratio in each brain area were significantly different in the mild intermittent hypoxia group, especially in the frontolobe cortex and hippocampus (P 0.05). In severe intermittent hypoxia group, the density of dead neurons and the ratio of Bcl-2/Bax in each brain area were significantly different, especially in cortex, hippocampus and striatum. Conclusion intermittent hypoxia can induce the differential expression of Bcl-2 and Bax and the loss of nerve cells in different brain regions, and the cortex, hippocampus and striatum are more sensitive to intermittent hypoxia injury.
【作者单位】：河北联合大学康复医学院;河北联合大学附属医院;
【基金】：河北省科技厅资助项目(No.09276103D-11)
【分类号】：R766

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