SN50对大鼠视网膜缺血再灌注损伤的保护作用

发布时间：2019-06-08 18:13

【摘要】：目的探讨核因子-κB(nuclear factor-κB,NF-κB)活性抑制剂SN50对大鼠视网膜缺血再灌注损伤(retinal ischemical reperfusion injury,RIRI)的保护作用。方法 27只成年雄性SD大鼠随机分为正常对照组、RIRI组、RIRI+SN50组,每组9只,建立RIRI动物模型,RIRI后6h、24h、72hHE染色观察大鼠视网膜病理变化,免疫组织化学检测NF-κB的表达,实时定量PCR检测TNF-α的表达。结果 RIRI组RIRI 6h后视网膜出现组织轻度水肿,少量细胞变性,随着时间的延长,视网膜的损伤逐渐加重;RIRI+SN50组视网膜的损伤明显减轻。RIRI后6h视网膜上可见NF-κB阳性表达,经过SN50注射处理后,NF-κB的阳性表达在RIRI后24h明显减弱;经过SN50注射处理后,与RIRI组比较,24h后视网膜的TNF-α表达差异有统计学意义(P0.05)。结论 SN50对RIRI具有保护作用。
[Abstract]:Objective to investigate the protective effect of SN50, an active inhibitor of nuclear factor-kappa B (nuclear factor- kappa B (NF- kappa B), on (retinal ischemical reperfusion injury,RIRI after retinal ischemia-reperfusion injury in rats. Methods Twenty-seven adult male SD rats were randomly divided into normal control group (n = 9), RIRI group (n = 9) and RIRI SN50 group (n = 9). The animal model of RIRI was established. At 24 h after RIRI, 72 h HE staining was used to observe the retinal pathological changes, and the expression of NF- 魏 B was detected by immunohistochemistry. The expression of TNF- 伪 was detected by real-time quantitative PCR. Results in RIRI group, the retinal tissue edema and a small number of cell degeneration occurred after 6 hours of RIRI. With the prolongation of time, the retinal injury became more and more serious. The retinal injury in RIRI SN50 group was significantly alleviated. The positive expression of NF- kappa B was observed at 6 h after RIRI. After SN50 injection, the positive expression of NF- 魏 B decreased significantly at 24 h after RIRI. After SN50 injection, compared with RIRI group, the expression of TNF- 伪 in retinal was significantly different after 24 hours (P 0.05). Conclusion SN50 has protective effect on RIRI.
【作者单位】：第二军医大学南京临床医学院(南京军区南京总医院)眼科;第二军医大学附属长征医院眼科;
【基金】：国家自然科学基金项目(编号:81200719)~~
【分类号】：R774.1

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