荧光定量RT-PCR检测鼻咽癌患者外周血微转移的研究
发布时间：2019-06-11 16:46
【摘要】： 目的 探讨荧光定量RT-PCR检测外周血CK14 mRNA、EGFR mRNA的表达在鼻咽癌患者外周血微转移诊断中的价值。 方法 筛选符合入组标准的鼻咽癌患者87例,经详细询问病史、认真查体、完善相关的影像学检查,治疗前1周内,由两名耳鼻咽喉头颈外科主治医师以上确定肿瘤TNM分期和临床分期,参照中医辨证分型标准确定中医证型,组织分化程度以病理科病理报告结果为准。采用荧光定量RT-PCR方法检测87例入组患者外周血CK14 mRNA、EGFR mRNA的表达情况,并分析其与肿瘤TNM分期、临床分期、组织分化程度及中医证型之间的关系。 结果 1、87例鼻咽癌患者外周血中CK14 mRNA、EGFR mRNA阳性表达率分别为64.4%(56/87)、58.6%(51/87),两者联合检测的阳性表达率为71.3%(62/87),5例远处转移鼻咽癌患者的外周血和5例肿瘤组织中均有表达(CK14 mRNA、EGFR mRNA阳性表达率为100%),而10例正常人外周血中未见表达； 2、鼻咽癌患者外周血CK14 mRNA、EGFR mRNA的阳性表达率与年龄、性别经统计学分析无关(P0.05),而与肿瘤的组织分化程度、临床分期有关(P0.05)； 3、鼻咽癌患者外周血中CK14 mRNA、EGFR mRNA的表达水平与T分期、N分期、临床分期有关(P0.05),分期越晚,外周血中CK14 mRNA、EGFR mRNA的表达水平越高； 4、鼻咽癌患者外周血中CK14 mRNA、EGFR mRNA的表达水平与组织分化程度有关(P0.05),低分化鳞癌外周血中CK14 mRNA、EGFR mRNA的表达水平高于高中分化鳞癌； 5、鼻咽癌患者的中医证型在不同临床分期上的分布具有统计学意义(P0.05),中医证型中气血凝结型主要集中在Ⅰ期,占54.5%(6/11),痰浊结聚型主要集中在Ⅱ期,占64%(16/25),火毒困结型主要集中在Ⅲ期,占61.5%(24/39),正虚毒滞型主要集中在Ⅳ期,占66.7%(8/12),且外周血CK14 mRNA、EGFR mRNA的表达水平与中医证型有关(P0.05),正虚毒滞型火毒困结型痰浊结聚型气血凝结型。 结论 1、荧光定量RT-PCR方法检测鼻咽癌患者外周血CK14 mRNA、EGFR mRNA的表达,能够较准确反映外周血微转移的情况,在诊断鼻咽癌患者外周血微转移方面有一定的可行性； 2、鼻咽癌患者外周血微转移与肿瘤的恶性程度和病情进展有关; 3、鼻咽癌患者中医辨证分型与临床分期关系密切,随着临床分期的进展,其中医证型呈现出“气血凝结型→痰浊结聚型→火毒困结型→正虚毒滞型”总的演变趋势,且随着中医证型的演变,外周血中CK14 mRNA、EGFR mRNA的表达水平逐渐升高。
[Abstract]:Objective to evaluate the value of fluorescence quantitative RT-PCR (FQ-RT-PCR) in the diagnosis of peripheral blood micrometastasis in patients with nasopharyngeal carcinoma (NPC). Methods 87 patients with nasopharyngeal carcinoma who met the criteria of admission were selected. After detailed medical history, careful physical examination and perfect related imaging examination, within one week before treatment, the TNM stage and clinical stage of the tumor were determined by two attending doctors of otorhinolaryngology head and neck surgery, and the TCM syndrome type was determined according to the TCM syndrome differentiation standard, and the degree of tissue differentiation was based on the pathological report results of pathology department. The expression of CK14 mRNA,EGFR mRNA in peripheral blood of 87 patients was detected by fluorescence quantitative RT-PCR, and the relationship between CK14 mRNA,EGFR mRNA expression and tumor TNM stage, clinical stage, degree of tissue differentiation and TCM syndrome type was analyzed. Results 1the positive expression rates of CK14 mRNA,EGFR mRNA in peripheral blood of 87 patients with nasopharyngeal carcinoma (NPC) were 64.4% (56%) and 58.6% (51%), respectively. The positive expression rates of CK14 mRNA,EGFR mRNA were 71.3% (62%) in combined detection, 100% (100%) in peripheral blood of 5 patients with distant metastasis of nasopharyngeal carcinoma (NPC), but no expression was found in 10 normal controls. 2. The positive expression rate of CK14 mRNA,EGFR mRNA in peripheral blood of patients with nasopharyngeal carcinoma (NPC) was not related to age and sex (P 0.05), but related to the degree of tumor differentiation and clinical stage (P 0.05). 3. The expression level of CK14 mRNA,EGFR mRNA in peripheral blood of patients with nasopharyngeal carcinoma was related to T stage, N stage and clinical stage (P 0.05). The later the stage, the higher the expression level of CK14 mRNA,EGFR mRNA in peripheral blood. 4. The expression level of CK14 mRNA,EGFR mRNA in peripheral blood of patients with nasopharyngeal carcinoma was related to the degree of tissue differentiation (P 0.05). The expression level of CK14 mRNA,EGFR mRNA in peripheral blood of poorly differentiated squamous cell carcinoma was higher than that of highly differentiated squamous cell carcinoma. 5. the distribution of TCM syndrome types in different clinical stages of nasopharyngeal carcinoma patients was statistically significant (P 0.05). The type of qi and blood coagulation was mainly in stage I, accounting for 54.5% (6 鈮，
本文编号：2497334
[Abstract]:Objective to evaluate the value of fluorescence quantitative RT-PCR (FQ-RT-PCR) in the diagnosis of peripheral blood micrometastasis in patients with nasopharyngeal carcinoma (NPC). Methods 87 patients with nasopharyngeal carcinoma who met the criteria of admission were selected. After detailed medical history, careful physical examination and perfect related imaging examination, within one week before treatment, the TNM stage and clinical stage of the tumor were determined by two attending doctors of otorhinolaryngology head and neck surgery, and the TCM syndrome type was determined according to the TCM syndrome differentiation standard, and the degree of tissue differentiation was based on the pathological report results of pathology department. The expression of CK14 mRNA,EGFR mRNA in peripheral blood of 87 patients was detected by fluorescence quantitative RT-PCR, and the relationship between CK14 mRNA,EGFR mRNA expression and tumor TNM stage, clinical stage, degree of tissue differentiation and TCM syndrome type was analyzed. Results 1the positive expression rates of CK14 mRNA,EGFR mRNA in peripheral blood of 87 patients with nasopharyngeal carcinoma (NPC) were 64.4% (56%) and 58.6% (51%), respectively. The positive expression rates of CK14 mRNA,EGFR mRNA were 71.3% (62%) in combined detection, 100% (100%) in peripheral blood of 5 patients with distant metastasis of nasopharyngeal carcinoma (NPC), but no expression was found in 10 normal controls. 2. The positive expression rate of CK14 mRNA,EGFR mRNA in peripheral blood of patients with nasopharyngeal carcinoma (NPC) was not related to age and sex (P 0.05), but related to the degree of tumor differentiation and clinical stage (P 0.05). 3. The expression level of CK14 mRNA,EGFR mRNA in peripheral blood of patients with nasopharyngeal carcinoma was related to T stage, N stage and clinical stage (P 0.05). The later the stage, the higher the expression level of CK14 mRNA,EGFR mRNA in peripheral blood. 4. The expression level of CK14 mRNA,EGFR mRNA in peripheral blood of patients with nasopharyngeal carcinoma was related to the degree of tissue differentiation (P 0.05). The expression level of CK14 mRNA,EGFR mRNA in peripheral blood of poorly differentiated squamous cell carcinoma was higher than that of highly differentiated squamous cell carcinoma. 5. the distribution of TCM syndrome types in different clinical stages of nasopharyngeal carcinoma patients was statistically significant (P 0.05). The type of qi and blood coagulation was mainly in stage I, accounting for 54.5% (6 鈮，
本文编号：2497334
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