P16、Ki-67、HMB45在人结膜恶性黑色素瘤的表达及与其临床分期、预后的相关性研究
发布时间：2019-06-14 00:25
【摘要】：目的：本研究旨在观察P16、Ki-67、HMB45在人结膜恶性黑色素瘤的表达情况，，并探究它们与结膜恶性黑色素瘤临床分期、预后的关系。 方法：对中山大学中山眼科中心2002年4月到2010年7月收治的20例结膜恶性黑色素瘤患者进行临床资料分析并对患者进行电话回访，观察其临床病理特点及分析免疫组化结果。同时收集20例结膜色素痣石蜡包埋标本作为实验对照组。采用免疫组织化学染色技术观察P16、Ki-67、HMB45在人结膜恶性黑色素瘤和结膜色素痣的表达情况。根据美国癌症联合协会分类法（AJCC）2010年关于结膜恶性黑色素瘤的分期标准进行临床分期，统计分析P16、Ki-67、HMB45与结膜恶性黑色素瘤临床分期、预后之间的关系。 结果：P16在结膜恶性黑色素瘤中的阴性表达率、低表达率、中高表达率分别为60%（12/20）、20%（4/20）、20%（4/20）；在结膜色素痣中的阴性表达率、低表达率、中高表达率分别为5%（1/20）、20%（4/20）、75%（15/20）。Ki-67在结膜恶性黑色素瘤中的阴性表达率、低表达率、中高表达率分别为20%（4/20）、25%（5/20）、55%（11/20）；在结膜色素痣中的阴性表达率、低表达率分别为75%（15/20）、25%（5/20）。HMB45在结膜恶性黑色素瘤中的阴性表达率、低表达率、中高表达率分别为10%（2/20）、15%（3/20）、75%（15/20）；在结膜色素痣中的阴性表达率、低表达率分别为90%（18/20）、10%（2/20）。P16、Ki-67、HMB45在结膜恶性黑色素瘤与结膜色素痣两组间的表达均有统计学差异（P＜0.05）。结膜恶性黑色素瘤中P16与Ki-67表达呈负相关（r=㧟0.786,P＜0.05）。P16与结膜恶性黑色素瘤临床分期、预后呈负相关（r=㧟0.618，P＜0.05；r=㧟0.491，P＜0.05）。Ki-67与结膜恶性黑色素瘤预后呈正相关（r=0.504，P＜0.05）。HMB45与结膜恶性黑色素瘤临床分期、预后无关（X2=2.629，P＞0.05；X2=0.317，P＞0.05）。 结论：P16在结膜恶性黑色素瘤中的表达低于结膜色素痣；HMB45、Ki-67在结膜恶性黑色素瘤中的表达高于结膜色素痣。结膜恶性黑色素瘤中P16与Ki-67的表达呈负相关。P16与结膜恶性黑色素瘤临床分期、预后呈负相关；Ki67与结膜恶性黑色素瘤预后呈正相关。提示P16和Ki-67可能对预测结膜恶性黑色素瘤的预后有重要意义。
[Abstract]:Objective: to observe the expression of P16, Ki67 and HMB45 in human conjunctival malignant melanoma and to explore their relationship with clinical stage and prognosis of conjunctival malignant melanoma. Methods: the clinical data of 20 patients with conjunctival malignant melanoma admitted to Zhongshan Ophthalmology Center of Sun Yat-sen University from April 2002 to July 2010 were analyzed and the patients were interviewed by telephone. The clinicopathological features and the results of immunohistochemistry were observed. At the same time, 20 paraffin embedded specimens of conjunctival pigmented nevi were collected as experimental control group. The expression of P16, Ki67 and HMB45 in human conjunctival malignant melanoma and conjunctival pigmented nevi was observed by immunohistochemical staining. According to the classification of the American Association of Cancer (AJCC) in 2010, the clinical staging of conjunctival malignant melanoma was carried out. The relationship between P16, Ki67, HMB45 and the clinical stage and prognosis of conjunctival malignant melanoma was statistically analyzed. Results: the negative expression rate and low expression rate of P16 in conjunctival malignant melanoma were 60% (12 鈮
本文编号：2498944
[Abstract]:Objective: to observe the expression of P16, Ki67 and HMB45 in human conjunctival malignant melanoma and to explore their relationship with clinical stage and prognosis of conjunctival malignant melanoma. Methods: the clinical data of 20 patients with conjunctival malignant melanoma admitted to Zhongshan Ophthalmology Center of Sun Yat-sen University from April 2002 to July 2010 were analyzed and the patients were interviewed by telephone. The clinicopathological features and the results of immunohistochemistry were observed. At the same time, 20 paraffin embedded specimens of conjunctival pigmented nevi were collected as experimental control group. The expression of P16, Ki67 and HMB45 in human conjunctival malignant melanoma and conjunctival pigmented nevi was observed by immunohistochemical staining. According to the classification of the American Association of Cancer (AJCC) in 2010, the clinical staging of conjunctival malignant melanoma was carried out. The relationship between P16, Ki67, HMB45 and the clinical stage and prognosis of conjunctival malignant melanoma was statistically analyzed. Results: the negative expression rate and low expression rate of P16 in conjunctival malignant melanoma were 60% (12 鈮
本文编号：2498944
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