慢性锰中毒对大鼠听功能及耳蜗细胞的影响

发布时间：2019-06-28 15:19

【摘要】：目的探讨锰中毒对大鼠听功能及耳蜗毛细胞、螺旋神经节细胞的影响。方法选取60只断乳21天的SD大鼠,随机分为染毒组和对照组,染毒组(30只)给予氯化锰水溶液(MnCl2·4H2O,100mg·kg-1d-1)连续灌胃12w,对照组(30只)以同法给予等量去离子水。于灌胃4、8和12w后分别检测两组大鼠血锰浓度,于灌胃12w对两组大鼠分别行ABR检测,基底膜铺片鬼笔环肽-异硫氰酸荧光素(Phalloidin-FITC)染色观察毛细胞形态及计数,HE染色观察螺旋神经节细胞形态及计数,透射电镜观察毛细胞及螺旋神经节细胞超微结构的变化。结果染毒组大鼠灌胃4、8、12w后血锰浓度分别为24.1±2.4、31.5±2.6、26.7±2.8ng/ml,明显高于对照组(分别为8.5±1.7、11.2±2.1、12.7±2.1ng/ml),差异有统计学意义(均为P0.05)。灌胃12w后,染毒组大鼠4、8、16、24、32kHz ABR反应阈均明显高于对照组(P0.05),其中高频阈值升高显著。灌胃12w后,染毒组耳蜗毛细胞缺失,以底回缺失明显;螺旋神经节细胞数量减少;毛细胞和螺旋神经节细胞线粒体嵴断裂或消失,呈空泡样变。结论慢性锰中毒可导致大鼠耳蜗毛细胞缺失和螺旋神经节细胞减少,进而导致大鼠听功能损伤。
[Abstract]:Objective to investigate the effects of manganese poisoning on auditory function, cochlear hair cells and spiral ganglion cells in rats. Methods Sixty SD rats weanned for 21 days were randomly divided into two groups: control group (n = 30) and control group (n = 30). The rats in the exposed group (n = 30) were treated with MnCl2 路4H2O (100mg kg-1d-1) for 12 weeks, and the control group (n = 30) was treated with the same amount of deionized water. The blood manganese concentrations of the two groups were measured at 4, 8 and 12 weeks after intragastric administration. ABR was detected in the two groups at 12 weeks. The morphology and count of hair cells were observed by Phalloidin-FITC staining, the morphology and counting of spiral ganglion cells were observed by HE staining, and the ultrastructure of hair cells and spiral ganglion cells were observed by transmission electron microscope. Results the blood manganese concentrations in the exposed group were 24.1 卤2.4, 31.5 卤2.6 and 26.7 卤2.8 ng 路ml, respectively, which were significantly higher than those in the control group (8.5 卤1.7, 11.2 卤2.1, 12.7 卤2.1 ng/ml, respectively). After 12 weeks of intragastric administration, the ABR reaction threshold of 4, 8, 16, 24 and 32 ABR in the exposed group was significantly higher than that in the control group (P 0.05), and the high frequency threshold was significantly higher than that in the control group. After 12 weeks of intragastric administration, the hair cells of cochlea in the exposed group were absent, especially in the basal gyrus, the number of spiral ganglion cells decreased, and the mitochondrial cristae of hair cells and spiral ganglion cells were broken or disappeared, showing vacuole-like changes. Conclusion chronic manganese poisoning can lead to the loss of hair cells and the decrease of spiral ganglion cells in the cochlea of rats, which leads to the damage of auditory function in rats.
【作者单位】：第四军医大学西京医院耳鼻咽喉头颈外科;解放军后勤学院门诊部;
【基金】：国家自然科学基金项目(H2602H2607)
【分类号】：R764

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