小鼠急性细菌性鼻—鼻窦炎模型

发布时间：2019-07-08 09:11

【摘要】： 目的建立小鼠急性细菌性鼻-鼻窦炎模型。方法 179只BALB／c小鼠,随机分为4组。A,B组BALB／c小鼠右侧鼻腔中塞入棉条。A组中,以耐甲氧西林金黄色葡萄球菌(MRSA)COL菌悬液浸润棉条；B组则以无菌生理盐水浸润棉条。C组,只在小鼠鼻腔中滴入MRSA COL菌悬液。D组为对照组。动物分别于1,4,7,14天处死。对鼻部组织做细菌培养和病理切片研究。死亡的3只小鼠(A组)未计入数据统计中。结果 A组小鼠全部诱导出急性细菌性鼻-鼻窦炎,B组小鼠可诱导出鼻腔炎症反应。炎症程度经统计学分析,A、B组有明显统计学差异。C,D组小鼠没有出现炎症反应。结论：成功建立小鼠急性细菌性鼻-鼻窦炎模型
文内图片：黑色表示MRSA在A组小鼠中的定植情况，白色代表C组

图片说明：黑色表示MRSA在A组小鼠中的定植情况，，白色代表C组
[Abstract]:Objective to establish a mouse model of acute bacterial rhinosinusitis. Methods 179 BALB/c mice were randomly divided into 4 groups. Group A and group B BALB/c mice were inserted into the right nasal cavity. Group A was infiltrated with methicillin-resistant Staphylococcus aureus (MRSA) COL suspension and group B was infiltrated with aseptic saline. Group C was only infused with MRSA COL bacteria suspension in the nasal cavity of mice. Group D was used as the control group. The animals were executed at 1, 4, 7 and 14 days respectively. The bacterial culture and pathological section of nasal tissue were studied. Three dead mice (group A) were not included in the data statistics. Results Acute bacterial rhinosinusitis was induced in all mice in group A, and inflammation in nasal cavity was induced in group B. There was significant difference in the degree of inflammation between group A and group B. there was no inflammatory reaction in group C and D. Conclusion: the mouse model of acute bacterial rhinosinusitis was successfully established.
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R765.41

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