亲和毛细管整体柱在牛血清白蛋白与奈福泮对映体相互作用研究中的应用

发布时间：2018-01-02 04:12

  本文关键词：亲和毛细管整体柱在牛血清白蛋白与奈福泮对映体相互作用研究中的应用　出处：《中国药科大学学报》2016年01期 　论文类型：期刊论文

  更多相关文章： 亲和毛细管整体柱 手性药物 牛血清白蛋白 相互作用 奈福泮 前沿分析法

【摘要】：利用胃蛋白酶键合的有机聚合物整体柱在电色谱中对手性药物奈福泮的拆分能力,采用前沿分析法同时对奈福泮的两个对映体与牛血清白蛋白(BSA)的相互作用情况进行了考察。经过优化后建立的电色谱条件为:胃蛋白酶修饰的聚(甲基丙烯酸环氧丙酯-乙二醇二甲基丙烯酸酯)毛细管整体柱作为分离通道(32 cm×75μm,有效长度22 cm),运行缓冲液为pH 5.5的15 mmol/L醋酸铵,样品溶剂为pH 7.4的50 mmol/L醋酸铵,运行电压为5.0 kV,电压进样10 kV×6 s,检测波长为215 nm。此时奈福泮两个对映体平台峰彼此完全分离,结合体系中BSA对对映体在电色谱中的分离和检测均无影响,测得两个对映体与BSA的结合常数分别为443和527 L/mol,结合位点数均为1.0,结合位点为Sudlow siteⅡ。
[Abstract]:The separation ability of using organic polymer monolithic column bonded in pepsin electrochromatography chiral drugs of nefopam, by frontal analysis of nefopam two enantiomers with bovine serum albumin (BSA) interactions were investigated. After power optimized chromatographic conditions established: pepsin modification poly (methacrylic acid ethylene glycol two glycidyl methacrylate) monolithic capillary column as the separation channel (32 cm * 75 m, the effective length of 22 cm), the running buffer was pH 5.5 15 mmol/L ammonium acetate, the sample solvent for pH 7.4, 50 mmol/L ammonium acetate, the operating voltage is 5 kV, voltage. 10 kV * 6 s, the detection wavelength was 215 nm. when nefopam two enantiomers were separated completely from each other with the peak platform, in the system of BSA had no effect on the separation and determination of enantiomers in electro chromatography, measured two enantiomers and combined with BSA The constants are 443 and 527 L/mol respectively, and the number of binding sites is 1, and the binding site is Sudlow site II.

【作者单位】： 中国药科大学分析化学教研室;
【分类号】：R917
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