载血卟啉单甲醚纳米高分子微球的制备及显像实验

发布时间：2018-01-05 21:32

本文关键词：载血卟啉单甲醚纳米高分子微球的制备及显像实验　出处：《中国医学影像学杂志》2016年08期 　论文类型：期刊论文

【摘要】：目的制备出一种载血卟啉单甲醚(HMME)的聚乳酸-羟基乙酸(PLGA)多功能造影剂(MB_(PLGA-HMME),MB:Microbubble),检测MB_(PLGA-HMME)的基本性质以及HMME包裹在PLGA微球上后,性质改变情况,探讨其集显像与治疗一体化的可行性。资料与方法采用双乳化法制备MB_(PLGA-HMME),对其结构、形态、载药率等性质进行检测,采用体外成像比较HMME药物与MB_(PLGA-HMME)超声/光声成像改变情况以及进行初步体内光声成像。结果 MB_(PLGA-HMME)溶于双蒸水后呈淡红色悬液,显微镜下观察MB_(PLGA-HMME)呈球形,形态规则,大小均匀无聚集、粘连现象,能发出红色荧光,HMME均匀分布在微球上,光学显微镜和透射电镜均可见MB_(PLGA-HMME)为壳核结构,外有一层明显的黑色的壳膜,HMME主要分布于PLGA微球壳上。体外光声成像显示,MB_(PLGA-HMME)溶液浓度为408μmol/L、816μmol/L、1632μmol/L的平均光声信号强度分别为0.2009±0.0636、0.3789±0.0431、0.5380±0.0997;同样浓度梯度下,HMME溶液的平均光声信号值分别为0.0738±0.0133、0.1137±0.0065、0.2170±0.0270,组间和组内两两比较,差异有统计学意义,但药物性质基本未改变。MB_(PLGA-HMME)经静脉注入荷瘤鼠体内后,可进行体内光声显像。结论本研究成功制备载血卟啉单甲醚药物的MB_(PLGA-HMME),HMME药物水溶性得到改善,其基本的吸光性质得以保持,可进行体内外超声/光声成像。
[Abstract]:Objective to prepare a multifunctional porphyrin loaded porphyrin monomethyl ether (HMME-HMME-PLGA) multifunctional contrast medium (MBGA-HMME-PLGA-HMME). MBW: microbubble, to test the basic properties of HMME PLGA-HMME. and the change of nature when HMME is wrapped on PLGA microspheres. Methods MBSP PLGA-HMMEM was prepared by double emulsification method, and its structure, morphology and drug loading rate were tested. In vitro imaging was used to compare the changes in ultrasound / photoacoustic imaging between HMME drugs and HMME PLGA-HMMEs and to perform preliminary in vivo photoacoustic imaging. Dissolved in double steamed water, red suspension. Under the microscope, MBAP-HMME-PLGA-HMMEwas spherical, regular in shape, uniform in size, no aggregation in size, adhesion phenomenon, and HMME distributed evenly on the microspheres with red fluorescence. Both optical microscope and transmission electron microscope showed that MBAP-HMME-PLGA-HMME-PLGA-HMME-PLGA-HMMEwas a shell core with an obvious black shell film outside. HMME was mainly distributed on PLGA microsphere shell. In vitro photoacoustic imaging showed that the concentration of PLGA-HMME solution was 408 渭 mol / L ~ (-8) 渭 mol / L ~ (-8) 渭 mol/L. The average photoacoustic signal intensity of 1632 渭 mol/L was 0.2009 卤0.0636U 0.3789 卤0.0431U 0.5380 卤0.0997; Under the same concentration gradient, the average photoacoustic signal values of HMME solution were 0.0738 卤0.0133U 0.1137 卤0.0065U 0.2170 卤0.0270, respectively. There was significant difference between the two groups, but the nature of the drug was not changed. The PLGA-HMMEs were injected intravenously into the tumor-bearing mice. Conclusion the preparation of hematoporphyrin monomethyl ethers by MBSP PLGA-HMMEE can improve the water solubility of HMME. Its basic absorptivity can be preserved and ultrasound / photoacoustic imaging can be carried out in vivo and in vitro.
【作者单位】：重庆医科大学生物医学工程学院;重庆医科大学附属第二医院;
【基金】：国家973计划项目(N2011CB707900) 国家自然科学基金(81127901,11274404,81201102)
【分类号】：R943;R96
【正文快照】： 血卟啉单甲醚(hematoporphyrin monomethyl ether,HMME)是光动力疗法和声动力疗法最常用的光敏剂和声敏剂[1-4],具有组成单一、性能稳定、肿瘤选择性高、对正常组织光毒性低等优点,是一种具有广阔前景的药物。但也存在副反应,如色素沉着、瘙痒、局部疼痛等,并且难溶于水,在水

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