7-O-丁二酰大环内酯菌素A对人肺癌细胞H460的抑制作用及其机制

发布时间：2018-01-14 17:22

本文关键词：7-O-丁二酰大环内酯菌素A对人肺癌细胞H460的抑制作用及其机制　出处：《中国药科大学学报》2017年01期 　论文类型：期刊论文

【摘要】：探讨7-O-丁二酰大环内酯菌素A对人肺癌细胞侵袭转移的抑制作用及其机制。7-O-丁二酰大环内酯菌素A处理人肺癌细胞H460后,采用MTS、细胞黏附、Transwell和划痕实验观察7-O-丁二酰大环内酯菌素A对细胞生长、体外黏附能力及侵袭转移的影响,流式细胞术检测细胞周期和凋亡的变化,RT-PCR和Western blot检测7-O-丁二酰大环内酯菌素A对β-catenin、c-Myc、Cyclin D1、vimentin、N-cadherin、CD44、integrinβ1、bcl-2、Survivin和MMP-2/9的mRNA和蛋白表达的影响以及AKT和m TOR的磷酸化的变化。结果表明:7-O-丁二酰大环内酯菌素A显著抑制H460细胞的体外增殖和体外黏附,诱导细胞发生凋亡,且抑制细胞体外迁移和侵袭能力。Western blot和Real-time PCR结果显示,7-O-丁二酰大环内酯菌素A下调细胞中Bcl-2、Survivin、β-catenin、c-Myc、Cyclin D1、vimentin、N-cadherin、CD44、integrinβ1和MMP-2/9的表达,以及AKT和m TOR的磷酸化。7-O-丁二酰大环内酯菌素A可抑制人肺癌细胞H460的体外生长,并抑制其体外黏附和侵袭转移能力。
[Abstract]:The inhibitory effect and mechanism of.7-O- Ding two macrolide bacteria of acyl 7-O- Ding two macrolides by cyclosporin A on invasion and metastasis of human lung cancer cells treated with mitomycin A in human lung cancer cells H460, using MTS, cell adhesion, observation of 7-O- Ding two macrolide antibiotic acyl A on the growth of Transwell cells and scratch test effect of adhesion and invasion in vitro and metastasis, cell cycle and apoptosis were detected by flow cytometry, RT-PCR and Western blot to detect 7-O- Ding two macrolides by cyclosporin A on beta -catenin, c-Myc, Cyclin, D1, vimentin, N-cadherin, CD44, integrin beta 1, Bcl-2, mRNA and Survivin protein expression and MMP-2/9 and the changes of AKT and M phosphorylation of TOR. The results showed that the proliferation and adhesion in vitro 7-O- Ding two acyl macrolide streptogramin A significantly inhibited H460 cells in vitro, induce apoptosis and inhibit cell invasion and migration ability of.Western bl OT and Real-time PCR showed that 7-O- Ding two macrolide antibiotic A was down Bcl-2 in cells, Survivin, c-Myc, Cyclin beta -catenin, D1, vimentin, N-cadherin, CD44, the expression of integrin beta 1 and MMP-2/9, and the growth of phosphorylated.7-O- acyl Ding two macrolide strains AKT and m in TOR A can inhibit human lung cancer cells H460 in vitro, and inhibit the in vitro adhesion and invasion and metastasis.

【作者单位】：天津市中医药研究院附属医院药剂科;天津市中医药研究院附属医院疮疡科;
【分类号】：R96
【正文快照】： 引用本文杨培树,郝文立.7-O-丁二酰大环内酯菌素A对人肺癌细胞H460的抑制作用及其机制[J].中国药科大学学报,2017,48(1):82-88.Cite this article as:YANG Peishu,HAO Wenli.Inhibitory effect and its mechanism of 7-O-succinyl macrolactin A against cell proliferation,i

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