大鼠氯霉素4周经口连续染毒毒性的研究

发布时间：2018-01-14 19:17

本文关键词：大鼠氯霉素4周经口连续染毒毒性的研究　出处：《环境与职业医学》2017年06期 　论文类型：期刊论文

【摘要】：[目的]研究氯霉素大鼠4周(28 d)经口染毒毒性,为其安全性评价提供毒理学相关参数。[方法]80只健康Wistar大鼠雌雄各半,随机分为1个对照组(5 g/L羧甲基纤维素钠溶液)和3个染毒组(高、中、低剂量组,氯霉素染毒剂量分别为60.0、30.0、7.5 mg/kg),每组20只,经口染毒,连续28 d。观察氯霉素对大鼠体重、血液指标及脏器系数的影响,并进行骨髓涂片及组织病理学检查。[结果]与对照组相比,高剂量组雄性大鼠第2周起体重增长受到抑制(P0.05),雌、雄大鼠平均红细胞容积、总蛋白、白蛋白降低(P0.05),雄性大鼠脑脏器系数与雌、雄大鼠肾脏脏器系数升高(P0.05);中剂量组雌、雄大鼠平均红细胞容积、总蛋白、白蛋白降低(P0.05),雄性大鼠肾脏脏器系数升高(P0.05);低剂量组大鼠各指标均未见明显改变。本试验条件下,大鼠氯霉素连续28 d经口染毒未观察到损害作用剂量为7.5 mg/kg,基准剂量为1.284 mg/kg,基准剂量下限为0.804 mg/kg,人类每日容许摄入量为8.04μg/kg。[结论]氯霉素对雄性大鼠的毒性大于雌性大鼠。本研究为氯霉素每日摄入量安全限值的制定及风险评估提供了重要的数据参考。
[Abstract]:[Objective] to study the chloramphenicol in rats for 4 weeks (28 d) by oral administration for its toxicity, safety evaluation parameters. Provide toxicological methods]80 healthy Wistar rats of male and female, were randomly divided into 1 groups (5 g/L carboxymethyl cellulose sodium solution) and 3 groups (high. The low dose group, chloramphenicol dose were 60.0,30.0,7.5 mg/kg), 20 rats in each group, by oral administration, 28 d. continuous observation of chloramphenicol on rat body weight, blood index and organ coefficient, and bone marrow smear and histopathologic examination. Results compared with the control group, the high dose group of male rats for second weeks the weight of growth inhibition (P0.05), female, male rats mean corpuscular volume, total protein, albumin decreased (P0.05), male and female rat brain organ coefficient, kidney coefficient increased male rats (P0.05); the middle dose group of female and male rats in the mean corpuscular volume, total protein. Egg white White (P0.05), elevated decreased in male rats kidney organ coefficient (P0.05); the index of low dose group rats showed no obvious change. Under the experimental conditions, the rats for 28 d by oral administration of chloramphenicol was observed damage dose was 7.5 mg/kg, the benchmark dose is 1.284 mg/kg, the benchmark dose limit of 0.804 mg/kg, the human daily intake was 8.04 g/kg.[Conclusion] chloramphenicol toxicity in male rats than in female rats. The study provides important reference data for chloramphenicol daily intake safety limit setting and risk assessment value.

【作者单位】：上海市食品药品检验所药理毒理室药物安全性评价中心;
【基金】：上海市科学技术委员会科研计划项目(编号:12391901300)
【分类号】：R965
【正文快照】： [结果]与对照组相比,高剂量组雄性大鼠第2周起体重增长受到抑制(P0.05),雌、雄大鼠平均红细胞容积、总蛋白、白蛋白降低(P0.05),雄性大鼠脑脏器系数与雌、雄大鼠肾脏脏器系数升高(P0.05);中剂量组雌、雄大鼠平均红细胞容积、总蛋白、白蛋白降低(P0.05),雄性大鼠肾脏脏器系

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