CKLF1-C19对昆明小鼠的急性毒性研究

发布时间：2018-01-15 01:19

本文关键词：CKLF1-C19对昆明小鼠的急性毒性研究　出处：《中国比较医学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的研究趋化素样因子1(CKLF1)的多肽CKLF1-C19对昆明小鼠的急性毒性作用。方法以25m L/kg的CKLF1-C19(100μg/m L、1 mg/m L、10 mg/m L三种浓度)剂量注射实验组小鼠,对照组则给予同等体积生理盐水,给药后隔天记录小鼠的体重变化,密切观察实验组小鼠的一般状况。结束实验后留取小鼠腹主动脉血检测肝肾功能;留取肝、肾、脾、肺组织观察外观、做HE染色及常规组织病理学检查。结果两组小鼠一般状况良好,体重缓慢增长,两组小鼠体重变化差异无显著性(P0.05),14 d后均无死亡情况;两组小鼠肝肾功能相关的血液生化结果差异无显著性(P0.05);肉眼观察两组小鼠心脏、肝、肾、脾、肺等脏器正常,肝、肾、脾、肺组织HE染色后结构清晰,未见异常。结论 CKLF1-C19对小鼠无急性毒性作用。
[Abstract]:Objective to study the chemokine like factor 1 (CKLF1) the acute toxic effect of polypeptide CKLF1-C19 on Kunming mice. Methods 25m L/kg CKLF1-C19 (100 g/m L, 1 mg/m L, 10 mg/m L three concentration) dose injection in the experimental group, the control group were given an equal volume of saline, the next day to weight change mice were recorded after the treatment, close observation of general condition of experimental mice. The mice with abdominal aortic blood tests of liver and kidney function after the end of the experiment; liver, kidney, spleen, lung tissue visual observation, HE staining and histopathological examination. The general situation of two groups of mice, weight slow growth the difference of weight change of mice in two groups had no significant difference (P0.05), 14 d were no deaths; blood biochemical results of the two groups of mice liver and kidney function related to no significant difference (P0.05); the two groups of mice heart, liver and kidney, eye, spleen, lung and other organs of normal liver, kidney and spleen, After HE staining, the structure of lung tissue was clear and no abnormality was found. Conclusion CKLF1-C19 has no acute toxic effect on mice.

【作者单位】：广州医科大学附属第一医院呼吸疾病国家重点实验室;广州医科大学附属第一医院检验科;广州医科大学附属第一医院病理科;
【基金】：广州呼吸疾病国家重点实验室开放课题项目(编号:2014SKLRD-O04)
【分类号】：R965
【正文快照】： 趋化素样因子1(chemokine-like factor 1,CKLF1)是我国北京大学人类疾病基因研究中心在国际上首次报道的一个新的人类细胞因子[1],具有自主知识产权。CKLF1具备趋化因子CC亚家族的特征性结构[1],含有两个CKLF1的分泌型多肽CKLF1-C19和C27[2]。CKLF1及其两个多肽的功能性受体都

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