HO-1在顺铂诱导豚鼠螺旋神经元损伤中的作用

发布时间：2018-01-16 10:34

本文关键词：HO-1在顺铂诱导豚鼠螺旋神经元损伤中的作用　出处：《中华耳科学杂志》2015年01期 　论文类型：期刊论文

【摘要】：目的检测血红素加氧酶-1在豚鼠耳蜗螺旋神经节顺铂损伤后的表达变化。方法通过免疫组织化学及Western Blot技术,观察耳蜗螺旋神经元损伤后HO-1在不同组间的表达变化。将32只健康豚鼠随机分为4组,每组8只。A组对照组:按体重以0.9%生理盐水12ml/kg腹腔注射,早晚各一次,持续3天。B组顺铂组:按体重12mg/kg顺铂腹腔注射(IP),早晚各一次,持续3天。C组阿魏酸(FA)+顺铂组:先150mg/kg FA腹腔注射预处理1h,再以12mg/kg顺铂IP,早晚各一次,持续三天。D组FA+锌原卟啉Ⅸ(ZnppⅨ)+顺铂组:同时给予150mg/kg FA+ZnppⅨ15umol/kg IP预处理1h,再以12mg/kg顺铂IP,早晚各一次,持续三天。采用免疫组织化学及Wstern Blot技术检测不同组间螺旋神经元HO-1蛋白表达变化。结果在不同的分组中,HO-1在耳蜗螺旋神经节中表达量不同。空白组中,螺旋神经节组织存在微弱的HO-1表达,其蛋白表达量为0.48±0.07;A组蛋白表达量为0.81±0.07,B组蛋白表达量为0.9±0.05,C组蛋白表达量为0.61±0.07。4个组两两间差异均有统计学意义(p0.0001)。结论 HO-1在C组中表达量最多,B组次之,D组最少,表明HO-1可能参与顺铂损伤螺旋神经元后的修复过程。
[Abstract]:Objective to detect the expression of heme oxygenase-1 in cochlear spiral ganglion of guinea pig after cisplatin injury. Methods Immunohistochemistry and Western Blot technique were used to detect the expression of heme oxygenase-1 in guinea pig cochlear spiral ganglion. To observe the expression of HO-1 in different groups after cochlear spiral neuron injury, 32 healthy guinea pigs were randomly divided into 4 groups. Each group of 8 rats in the control group: according to the body weight of 0.9% normal saline 12 ml / kg intraperitoneal injection, morning and evening each time. Group B: Cisplatin was injected intraperitoneally according to body weight of 12 mg / kg cisplatin for 3 days, once in the morning and evening. Cisplatin group: 150 mg / kg FA was injected intraperitoneally for 1 hour, then 12 mg / kg Cisplatin IPP was given once in the morning and evening. For three days, group D (FA zinc protoporphyrin IX ZnPP IX) cisplatin group was pretreated with 150 mg / kg FA Znpp IX 15 umol / kg IP for 1 h at the same time. And 12 mg / kg of cisplatin IPs, one in the morning and one in the morning. The changes of HO-1 protein expression in different groups were detected by immunohistochemistry and Wstern Blot technique for three days. The expression of HO-1 in the cochlear spiral ganglion was different. In the blank group, there was a weak expression of HO-1 in the spiral ganglion, and its protein expression was 0.48 卤0.07; The expression of group A protein was 0.81 卤0.07 and that of group B was 0.9 卤0.05. The expression of HO-1 in group C was 0.61 卤0.07.4.There was significant difference between two groups (P 0.0001). Conclusion the expression of HO-1 in group C is the most than that in group B and group D is the least. The results suggest that HO-1 may be involved in the repair of spiral neurons injured by cisplatin.
【作者单位】：重庆医科大学附属第二院耳鼻咽喉科;
【基金】：重点项目(2012-1-041)
【分类号】：R965
【正文快照】： 顺铂作为一种临床上常用的广谱抗癌药,被广泛用于治疗各种恶性肿瘤。但因顺铂有严重的耳毒性副作用,重则造成双侧听力完全损失,这成为影响该药治疗乃至停药的重要因素。研究已证实铂类药物对内耳中螺旋神经节的损伤机制主要是直接损伤,其中造成细胞内氧化/抗氧化失衡是顺铂损

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