具有氧化还原响应性的含硒小分子多肽自组装体系

发布时间：2018-01-16 12:00

本文关键词：具有氧化还原响应性的含硒小分子多肽自组装体系　出处：《南开大学》2014年硕士论文　论文类型：学位论文

【摘要】：从相对简单的系统,例如脂质体双分子层和DNA双螺旋结构,到复杂的结构器官,自组装广泛存在于自然界中。这些组装体中的大部分是动态的,它们的形成是可逆的。最近,越来越多的研究人员通过研究这些动态自组装体系和它们的可逆性质制备了一些人工智能材料。例如,光响应的智能材料,电响应的智能材料,化学因素响应的智能材料。其中,氧化还原体系(例如NADH/NAD)被大量应用去调控生物活性,例如高分子球和纳米凝胶的解组装以及生物活性分子的释放。受到这些启发,我们发展了具有氧化还原响应性的含硒多肽自组装体系,即含硒自组装多肽能被氧化成含硒亚砜的自组装多肽,又能被还原回来。同时,我们是利用维生素C(VC)将含有硒亚砜的自组装多肽还原成含硒多肽最终形成小分子多肽水凝胶,这是一种新的生物相容性好的形成小分子水凝胶的方法。我们进一步把组氨酸接到含硒的自组装多肽上,发现通过氧化还原可以调控含有组氨酸残基的多肽的催化活性。我们的结果证实了自组装的多肽的生物活性与多肽的自组装结构有关并且可以进行调控。具有氧化还原响应性的含硒自组装多肽的发展将会给模仿自然的人工智能材料的发展提供新的思路。
[Abstract]:From relatively simple systems, such as liposome bimolecular layers and DNA double helix structures to complex structural organs, self-assembly is widespread in nature. Most of these assemblies are dynamic. Their formation is reversible. Recently, more and more researchers have prepared artificial intelligence materials by studying these dynamic self-assembly systems and their reversible properties, for example, light-responsive smart materials. Smart material for electrical response and smart material for chemical response. Among them, redox systems (such as NADH / NAD) are widely used to regulate biological activities, such as the deassembly of polymer spheres and nanogels and the release of bioactive molecules. We have developed a selenium-containing polypeptide self-assembly system with redox response, that is, the selenium-containing self-assembled polypeptide can be oxidized to self-assembled polypeptide containing selenium sulfoxide, and can be reduced back at the same time. We reduce the self-assembled polypeptide containing selenium sulfoxide to the polypeptide containing selenium and form the hydrogel of small molecule polypeptide using vitamin C (VC). This is a new biocompatible method for the formation of small molecular hydrogels. We further linked histidine to selenium-containing self-assembled polypeptides. It is found that the catalytic activity of peptides containing histidine residues can be regulated by redox. Our results show that the biological activity of self-assembled peptides is related to the self-assembly structure of peptides and can be regulated by oxygen. The development of selenium-containing self-assembled polypeptides, which are responsive to reduction, will provide a new idea for the development of artificial intelligence materials that mimic nature.
【学位授予单位】：南开大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R914

【共引文献】