高效液相色谱法测定大鼠血浆和肺泡灌洗液中环丙沙星浓度

发布时间：2018-01-18 04:02

  本文关键词：高效液相色谱法测定大鼠血浆和肺泡灌洗液中环丙沙星浓度　出处：《中国临床药理学杂志》2017年09期 　论文类型：期刊论文

  更多相关文章： 高效液相色谱 环丙沙星 血药浓度 肺泡灌洗液

【摘要】：目的建立一种测定大鼠血浆和肺泡灌洗液中环丙沙星浓度的高效液相色谱(HPLC)法。方法用甲醇直接蛋白沉淀法、去离子水直接稀释法分别对血浆和肺泡灌洗液进行处理,离心取上清,以莫西沙星为内标,用HPLC测定。色谱柱:Agilent SB-C18柱(4.6 mm×150 mm,5μm),流动相:乙腈-0.1%甲酸水,流速:1.0 m L·min~(-1),柱温:25℃,进样量:15μL。考察该方法的专属性、标准曲线与最低定量下限、精密度与回收率、稳定性。结果血浆和肺泡灌洗液中的内源性物质不影响环丙沙星的测定。在血浆和肺泡灌洗液中,环丙沙星分别在1.00~100.00μg·m L~(-1)(r=0.999 6)、0.05~2.00μg·m L~(-1)(r=0.998 5)内线性关系良好,最低定量下限分别为1.00和0.05μg·m L~(-1)。环丙沙星在血浆和肺泡灌洗液中的批内精密度分别为0.49%~3.77%和2.21%~5.00%,批间精密度分别为7.72%~8.83%和6.84%~9.51%,提取回收率分别为104.61%~113.29%和100.65%~103.96%;环丙沙星血浆和肺泡灌洗液稳定性符合相关要求。结论该方法简单、准确、可操作性强,适用于伏立康唑大鼠血浆及肺泡灌洗液浓度测定。
[Abstract]:Objective to establish a high performance liquid chromatography (HPLC) method for the determination of ciprofloxacin in rat plasma and alveolar lavage fluid. Plasma and alveolar lavage fluid were treated by direct dilution of deionized water. The supernatant was centrifuged and moxifloxacin was used as the internal standard. HPLC was used to determine the content of acetonitrile-0.1% formic acid in the mobile phase. The chromatographic column was 4.6 mm 脳 150 mm ~ 5 渭 m 路m ~ (-1) with acetonitrile-0.1% formic acid water as the mobile phase. The flow rate is 1.0 mL 路min ~ (-1), the column temperature is 25 鈩，

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